Effects of Selenizing Codonopsis pilosula Polysaccharide on Macrophage Modulatory Activities

Qin, Tao; Ren, Zhe; Lin, Dandan; Song, Yulong; Li, Jian; Ma, Yufang; Hou, Xuehan; Huang, Yifan

doi:10.4014/jmb.1601.01094

Cited by 39 publications

(7 citation statements)

References 22 publications

(22 reference statements)

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“…It was reported that the selenized Cordyceps militaris polysaccharide (Se‐CPS) exhibited an absorption peak at 848.1 cm −1 , which was assigned to the C−O−Se symmetric stretching vibration [36] . These results were also similar to studies Qin et al., [37] as well as Liu et al., [38] who reported that peaks ascribed to C−O−Se stretching vibrations appeared at 668 and 665.38 cm −1 , respectively.…”

Section: Resultssupporting

confidence: 92%

Selenylation of Guar Gum Mediated by N‐Methyl‐2‐pyrrolidone Hydrosulfate: Insights into Regulation of Selenium Content and Molecular Weight

Zhou

et al. 2022

ChemistrySelect

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Selenylation is an important modification strategy of polysaccharide, however, one of the main obstacles is that the weak esterification ability of selenide reagent, which leads to the low Se conversion efficiency during reaction. Here, we used N-methyl-2-pyrrolidone hydrosulfate ([HNMP]HSO 4 ) as acid catalyst efficiently synthesized selenized guar gum (Se-GGs) to improve the activity of selenide reagent. The chemical structure of GG and selenized derivatives was investigated using FT-IR, 1D/2D NMR spectrometry and atomic force microscopy (AFM).The results indicated that selenite groups existed in Se-GGs and the substitution position was at C6. Furthermore, it was also demonstrated that [HNMP]HSO 4 had a dual influence on GG by increasing the Se content (from 181.3 to 9072.8 μg/g) and regulating the molecular weight, which was due to strong Brönsted acidic proton (H + ). These results illustrated a new synthetic method for preparing functional polysaccharides with a broad range of potential applications.

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Section: Resultssupporting

confidence: 92%

Selenylation of Guar Gum Mediated by N‐Methyl‐2‐pyrrolidone Hydrosulfate: Insights into Regulation of Selenium Content and Molecular Weight

Zhou

et al. 2022

ChemistrySelect

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“…Furthermore, the selenylated lentinan was more effective to inhibit the progress of inflammation and fibrosis, through enhancing superoxide dismutase and glutathione peroxidase activities, preventing lipid peroxidation, and decreasing the secretion levels of TNF-α and IL-1β, as well as pancreatic hydroxyproline in the 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC) mouse model [49]. It is well agreed that macrophages are professional phagocytes, and phagocytosis signifies the first and unique process in the immune response [50]. In addition, the proliferation of splenocytes reflects the status of the immune response level, while the reactive differentiation of T-lymphocytes is considered a key part of adaptive immunity [51].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Immunomodulation of the Polysaccharides from Yam (Dioscorea opposita Thunb.) in Response to a Selenylation of Lower Extent

Guan

Lin

et al. 2021

Foods

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The immunomodulation of chemically selenylated polysaccharides has been attracting more attention recently, but the corresponding performance of the yam polysaccharides (YPS) with lower selenylation extent remains, thus far, unsolved. In this study, the YPS was selenylated with Na2SeO3 under acidic conditions generated by HNO3 to reach two lower selenylation extents, yielding two selenylated YPSs, namely SeYPS-1 and SeYPS-2 with selenium contents of 715 and 1545 mg/kg, respectively. The results indicated that YPS, SeYPS-1, and SeYPS-2 all had in vitro immuno-modulation when using RAW 264.7 macrophages and murine splenocytes as cell models. In detail, the three polysaccharide samples at dose levels of 5–160 μg/mL showed insignificant cytotoxicity to the macrophages and splenocytes with cell exposure times of 12–24 h, because of the measured values of cell viability larger than 100%. However, Na2SeO3 at dose levels of 1.3–3.25 μg/mL mostly caused obvious cytotoxic effects on the cells, resulting in reduced cell viability values or cell death, efficiently. The results demonstrated that, compared with YPS, both SeYPS-1 and SeYPS-2 at a lower dose level (5 μg/mL) were more active at promoting phagocytosis activity, increasing the CD4+/CD8+ ratio of the T-lymphocyte sub-population in the murine splenocyte, improving cytokine secretion, including interleukin-6 (IL-6), IL-1β, and tumor necrosis factor-α in the macrophages, or increasing interferon-γ secretion, but suppressing IL-4 production in the splenocytes. Consistently, SeYPS-2 has more potential than SeYPS-1 at exerting these assessed bioactivities in the cells. Thus, we conclude that a chemical modification of YPS using trace element Se at a lower selenylation extent could bring about higher immunomodulatory activity towards macrophages and splenocytes, while selenylation extent of YPS is a critical factor used to govern the assessed activity changes of YPS.

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“…C. pilosula extracts are very complex, consisting of polysaccharides, saponins, sesquiterpenes, polyphenolic glycosides, alkaloids, polyacetylenes, and phytosteroids . Polysaccharides isolated from C. pilosula are one of the plant's important active constituents, with multiple biological activities, including antioxidant, anti‐cancer, and immunomodulatory properties . Given that C. pilosula endophytes are able to produce similar bioactive molecules as the plant itself, isolation and identification of C. pilosula endophytes could lead to the discovery of novel bioactive molecules.…”

Section: Introductionmentioning

confidence: 99%

Exopolysaccharides from a Codonopsis pilosula endophyte activate macrophages and inhibit cancer cell proliferation and migration

Chen

et al. 2018

Thoracic Cancer

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BackgroundExopolysaccharides with structural diversity have shown wide applications in biomaterial, food, and pharmaceutical industries. Herein, we isolated an endophytic strain, 14‐DS‐1, from the traditional medicinal plant Codonopsis pilosula to elucidate the characteristics and anti‐cancer activities of purified exopolysaccharides.MethodsHPLC and GC‐MS were conducted to purify and characterize the exopolysaccharides isolated from 14‐DS‐1. Quantitative RT‐PCR, cell migration assays, immunofluorescence staining, and flow cytometry analysis were conducted to investighate the biological activity of DSPS.ResultsWe demonstrated that exopolysaccharides isolated from 14‐DS‐1 (DSPS), which were predominately composed of six monosaccharides, showed anti‐cancer activities. Biological activity analysis revealed that exposure to DSPS induced macrophage activation and polarization by promoting the production of TNF‐α and nitric oxide. Further analysis revealed that DSPS treatment promoted macrophage infiltration, whereas cancer cell migration was suppressed. In addition, DSPS exposure led to S‐phase arrest and apoptosis in cancer cells. Immunofluorescence staining revealed that treatment with DSPS resulted in defects in spindle orientation and positioning.ConclusionThese findings thus suggest that DSPS may have promising potential in cancer therapy.

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Effects of Selenizing Codonopsis pilosula Polysaccharide on Macrophage Modulatory Activities

Cited by 39 publications

References 22 publications

Selenylation of Guar Gum Mediated by N‐Methyl‐2‐pyrrolidone Hydrosulfate: Insights into Regulation of Selenium Content and Molecular Weight

Selenylation of Guar Gum Mediated by N‐Methyl‐2‐pyrrolidone Hydrosulfate: Insights into Regulation of Selenium Content and Molecular Weight

In Vitro Immunomodulation of the Polysaccharides from Yam (Dioscorea opposita Thunb.) in Response to a Selenylation of Lower Extent

Exopolysaccharides from a Codonopsis pilosula endophyte activate macrophages and inhibit cancer cell proliferation and migration

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