Effects of Serial Expansion of Septal Chondrocytes on Tissue‐Engineered Neocartilage Composition

Homicz, Mark R.; Schumacher, Barbara L.; Sah, Robert L.; Watson, Deborah

doi:10.1067/mhn.2002.129730

Cited by 104 publications

(78 citation statements)

References 27 publications

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“…Stem cells possess two properties: the ability to self-renew or proliferate and the ability to differentiate into a vast repertoire of specialized cells; therefore the use of cell therapy for the repair of damaged tissues as bones, cartilage or skin with or without the support of scaffolds is well established, demonstrating good short to medium term outcomes either in vitro of in vivo [17]- [21]. However, it is clear the presence of inherent limitations associated with current procedures include the low cell yield from the tissues obtained, the invasiveness of the first stage of the procedure required to obtain the needed amount of tissue and, the site morbidity associated with the procedure [13] [17].…”

Section: Discussion Proliferation Of Mononucleated Cellsmentioning

confidence: 99%

Isolation and Characterization of Multipotent and Pluripotent Stem Cells from Human Peripheral Blood

Gargiulo¹,

Pham²,

Hai³

et al. 2015

SCD

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Stem cells are commonly classified based on the developmental stage from which they are isolated, although this has been a source of debate amongst stem cell scientists. A common approach classifies stem cells into three different groupings: Embryonic Stem Cells (ESCs), Umbilical Cord Stem Cells (UCBSCs) and Adult Stem Cells (ASCs), which include stem cells from bone marrow (BM), fat tissue (FT), engineered induced pluripotent (IP) and peripheral blood (PB). By definition stem cells are progenitor cells capable of self-renewal and differentiation hypothetically "ab infinitum" into more specialized cells and tissue. The main intent of this study was to determine and characterize the different sub-groups of stem cells present within the human PB-SCs that may represent a valid opportunity in the field of clinical regenerative medicine. Stem cells in the isolated mononucleated cells were characterized using a multidisciplinary approach that was based on morphology, the expression of stem cell markers by flowcytometry and fluorescence analysis, RT-PCR and the capacity to self-renew or proliferate and differentiate into specialized cells. This approach was used to identify the expression of hematopoietic, mesenchymal, embryonic and neural stem cell markers. Both isolated adherent and suspension mononucleated cells were able to maintain their stem cell properties during in-vitro culture by holding their capacity for proliferation and differentiation into osteoblast cells, respectively, when exposed to the appropriate induction medium.

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Section: Discussion Proliferation Of Mononucleated Cellsmentioning

confidence: 99%

Isolation and Characterization of Multipotent and Pluripotent Stem Cells from Human Peripheral Blood

Gargiulo¹,

Pham²,

Hai³

et al. 2015

SCD

View full text Add to dashboard Cite

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“…In addition, it has also been described that the use of autologous cells provides limited repair outcomes in the long term owing to their lower potential to undergo cell proliferation. 1 An alternative cell sources is therefore needed in order to overcome the many limitations of this technique.…”

mentioning

confidence: 99%

Human peripheral blood derived mesenchymal stem cells demonstrate similar characteristics and chondrogenic differentiation potential to bone marrow derived mesenchymal stem cells

Chong

Selvaratnam²,

Abbas

et al. 2011

Journal Orthopaedic Research

127

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The use of mesenchymal stem cells (MSCs) for cartilage repair has generated much interest owing to their multipotentiality. However, their significant presence in peripheral blood (PB) has been a matter of much debate. The objectives of this study are to isolate and characterize MSCs derived from PB and, compare their chondrogenic potential to MSC derived from bone marrow (BM). PB and BM derived MSCs from 20 patients were isolated and characterized. From 2 ml of PB and BM, 5.4 AE 0.6 million and 10.5 AE 0.8 million adherent cells, respectively, were obtained by cell cultures at passage 2. Both PB and BM derived MSCs were able to undergo tri-lineage differentiation and showed negative expression of CD34 and CD45, but positively expressed CD105, CD166, and CD29. Qualitative and quantitative examinations on the chondrogenic potential of PB and BM derived MSCs expressed similar cartilage specific gene (COMP) and proteoglycan levels, respectively. Furthermore, the s-GAG levels expressed by chondrogenic MSCs in cultures were similar to that of native chondrocytes. In conclusion, this study demonstrates that MSCs from PB maintain similar characteristics and have similar chondrogenic differentiation potential to those derived from BM, while producing comparable s-GAG expressions to chondrocytes. ß

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“…For this reason, attempts have been made to use chondrocytes or undifferentiated mesenchymal stem cells (MSCs) that are expanded in vitro in functional hyaline cartilage engineering to treat the defects. Due to the poor proliferative capacity and dedifferentiation of chondrocytes during in vitro expansion, MSCs are becoming an important cell source for articular cartilage tissue engineering (4,14,23,36).…”

mentioning

confidence: 99%

Electromagnetic fields enhance chondrogenesis of human adipose-derived stem cells in a chondrogenic microenvironment in vitro

Lin

et al. 2013

Journal of Applied Physiology

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Effects of Serial Expansion of Septal Chondrocytes on Tissue‐Engineered Neocartilage Composition

Abstract: The alterations of matrix composition produced by dedifferentiated chondrocytes may limit the mechanical stability of neocartilage constructs.

Cited by 104 publications

References 27 publications

Isolation and Characterization of Multipotent and Pluripotent Stem Cells from Human Peripheral Blood

Isolation and Characterization of Multipotent and Pluripotent Stem Cells from Human Peripheral Blood

Human peripheral blood derived mesenchymal stem cells demonstrate similar characteristics and chondrogenic differentiation potential to bone marrow derived mesenchymal stem cells

Electromagnetic fields enhance chondrogenesis of human adipose-derived stem cells in a chondrogenic microenvironment in vitro

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