Effects of Sesquiterpenes Isolated From Largehead Atractylodes Rhizome on Growth, Migration, and Differentiation of B16 Melanoma Cells

Yan, Yonghong; Chou, Gui‐Xin; Wang, Hui; Chu, Jian-Hong; Fong, Wang-Fun; Yu, Zhi‐Ling

doi:10.1177/1534735410378660

Cited by 42 publications

(15 citation statements)

References 32 publications

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“…ATR exhibits a wide range of biological and pharmacological activities, such as anti-inflammatory, neuroprotection and gastroprotective effects [ 5 – 7 ]. Among the three members of ATR, ATR-1 exerts the best anti-tumor activity [ 8 , 9 ]. For example, ATR-1 was able to trigger apoptosis and cell cycle arrest in melanoma cells as well as lung carcinoma cells in vitro [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Anti-tumor effects of Atractylenolide I on bladder cancer cells

Chen

et al. 2016

J Exp Clin Cancer Res

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BackgroundAtractylenolide I (ATR-1), an active component of Rhizoma Atractylodis Macrocephalae, possesses cytotoxicity against various carcinomas. However, little is known about the effects of ATR-1on bladder cancer. In the present study, the anti-tumor activity of ATR-1 was examined on bladder cancer cells both in vivo and in vitro.MethodsMTT assay was used to assess the cytotoxic effect of ATR-1. Cell cycle distribution and apoptosis levels were evaluated using flow cytometry. Western blotting assay was applied to measure the levels of proteins associated with the apoptotic pathway, cell cycle progression and PI3K/Akt/mTOR signaling pathway. Tumor models in nude mice were induced by injection of T-24 and 253J human bladder cancer cells.ResultsATR-1 inhibited bladder cancer cell proliferation, arrested cell cycle in G2/M phase through up-regulation of p21 and down-regulation of cyclin B1, CDK1 and Cdc25c. Meanwhile, ATR-1 also triggered cellular apoptosis depending on the activation of mitochondrial apoptotic pathway. Mechanism investigation indicated that ATR-1 exerts its anti-tumor effect also relies on the inhibition of PI3K/Akt/mTOR signaling pathway. Finally, mice studies showed that ATR-1 blocked the T-24 or 253J-induced xenograft tumor growth without noticeable toxicity.ConclusionsATR-1 may be served as a potential therapeutic agent for the treatment of bladder cancer.

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Section: Introductionmentioning

confidence: 99%

Anti-tumor effects of Atractylenolide I on bladder cancer cells

Chen

et al. 2016

J Exp Clin Cancer Res

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“…In our in vitro experiment, treatment with both URAM and FRAM resulted in a marked suppression of lipid accumulation in differentiated 3T3-L1 cells, which are widely used as a model of adipogenesis 55 due to their potential capacity for differentiation from fibroblasts to adipocytes 56 . Previous studies have reported that RAM and its active components, including atractylenolide I, atractylenolide II, and atractylon inhibit adipogenesis via the PI3K/Akt signaling pathway 20 57 . Notably, in our study, no marked difference in the degree of inhibition of lipid accumulation was observed between URAM and FRAM-treated cells, implying that fermentation-mediated constitutional changes did not improve the anti-adipogenic activity of RAM.…”

Section: Discussionmentioning

confidence: 99%

Fermented Rhizoma Atractylodis Macrocephalae alleviates high fat diet-induced obesity in association with regulation of intestinal permeability and microbiota in rats

Wang

Bose

Kim

et al. 2015

Sci Rep

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Accumulating evidence suggests the anti-inflammatory and anti-obesity activities of Rhizoma Atractylodis Macrocephalae (RAM). Here, we evaluated the anti-obesity impact of unfermented (URAM) versus fermented RAM (FRAM) using both in vitro and in vivo models. Both URAM and FRAM exhibited marked anti-inflammatory, anti-adipogenic, and anti-obesity activities, and modulation of the gut microbial distribution. However, FRAM, compared to URAM, resulted in more efficient suppression of NO production and normalization of transepithelial electrical resistance in LPS-treated RAW 264.7 and HCT 116 cells, respectively. Compared to URAM, FRAM more effectively reduced the adipose tissue weight; ameliorated the serum triglyceride and aspartate transaminase levels; restored the serum HDL level and intestinal epithelial barrier function in the LPS control group. The relative abundance of Bifidobacterium and Akkermansia as well as Bacteriodetes/Firmicutes ratio in the gut of the LPS control group was significantly enhanced by both URAM and FRAM. However, FRAM, but not URAM, resulted in a significant increase in the distribution of Bacteriodetes and Lactobacillus in the gut of the HFD + LPS group. Our results suggest that FRAM with probiotics can exert a greater anti-obesity effect than URAM, which is probably mediated at least in part via regulation of the intestinal microbiota and gut permeability.

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“…A potential cancer treatment is to promote cancer cell differentiation, and thereafter, to reduce its proliferative capacity: this approach is a novel therapeutic method aiming to modify tumor cells to a slower rate of proliferation, and thus which leads to the loss of its earlier neoplastic attributes [25,26]. In melanoma, the differentiation status of cells could be monitored by melanogenesis, which resulted in the conversion of l -tyrosine to l- dopa by tyrosinase [27]. Indeed, the levels of melanin and tyrosinase are being considered as indicators of differentiated melanoma cells.…”

Section: Discussionmentioning

confidence: 99%

“…Lupeol, a lupine-type triterpene from Danelion root, showed significant inhibition on melanoma cell growth both in vitro and in vivo: the effect was trigged by its induction on cell differentiation [31]. Ye [27] reported that three sesquiterpenes, isolated from the largehead atractylode rhizome, induced melanoma cell differentiation and inhibited cell migration through inactivating the signals of Ras/ERK MAPK (for AT-I and AT-II) and PI3K/AKT. Here, we isolated and identified several prenylated flavonoids including isoflavone, flavone, chalcone, and coumarin.…”

Section: Discussionmentioning

confidence: 99%

“…Measurement of tyrosinase activity was performed according to a previously published method with slight modifications [27]. In brief, cells were cultured in 6-wells/plate at a density of 2 × 10 5 cells/well, and treated with different concentrations of GUR flavonoids (0, 20, and 40 μM, respectively) or α-MSH (20 nM) for 48 h. After the treatment, the cells were washed with ice-cold PBS twice, lysed at 4 °C for 30 min in RIPA lysis buffer (150 mMNaCl, 1 mM EDTA, 1 mM EGTA, 50 mM Tris–Cl, 0.35% w / v sodium-deoxycholate, 1 mM phenylmethylsulfonyl fluoride, 1% v / v NP-40, 1 mM NaF, 1 mM Na 3 VO 4 , pH 7.4) containing a protease-inhibitor cocktail.…”

Section: Methodsmentioning

confidence: 99%

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Prenylated Flavonoids from Roots of Glycyrrhiza uralensis Induce Differentiation of B16-F10 Melanoma Cells

Zheng

Wang

Yang

et al. 2018

IJMS

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Roots of Glycyrrhiza uralensis have been used as herbal medicine and natural sweetener. By activity-guided phytochemical investigation of the extracts from G. uralensis root, ten flavonoids, namely GF-1–GF-10, of which five were prenylated flavonoids, were found to show antiproliferative effects in melanoma B16-F10 cells. Three of the prenylated flavonoids, namely GF-1, GF-4 and GF-9, significantly induced the differentiation of B16-F10 cells; the inductions included increase of tyrosinase activity, tyrosinase protein, and melanin content. In GF-1 and GF-9 induced melanoma differentiation, the phosphorylation of p38 MAPK (mitogen activated potein kinase) was identified; while GF-4 could trigger the phosphorylation of PI3K/AKT (phosphatidylinositol 3-kinase/Protein Kinase B) signaling. However, application of GF-6 to the melanoma cells did not induce differentiation; but which promoted cell apoptotic signaling, i.e., increase levels of cleaved-PRAP, cleaved-caspase 3, and cleaved-caspase 9. These results suggested that different types of prenylated flavonoids from G. uralensis might have potential anticancer effects against melanoma cells by acting through different signaling pathways.

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Effects of Sesquiterpenes Isolated From Largehead Atractylodes Rhizome on Growth, Migration, and Differentiation of B16 Melanoma Cells

Cited by 42 publications

References 32 publications

Anti-tumor effects of Atractylenolide I on bladder cancer cells

Anti-tumor effects of Atractylenolide I on bladder cancer cells

Fermented Rhizoma Atractylodis Macrocephalae alleviates high fat diet-induced obesity in association with regulation of intestinal permeability and microbiota in rats

Prenylated Flavonoids from Roots of Glycyrrhiza uralensis Induce Differentiation of B16-F10 Melanoma Cells

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