Effects of Shock Waves on Oxidative Stress, Antioxidant Enzyme and Element Levels in Kidney of Rats

Abstract

The aim of this study was to investigate the effect of extracorporeal shock wave lithotripsy (ESWL) on kidney oxidative stress and trace element levels of adult rats. Twelve male Wistar albino rats were divided equally into two groups. First group was used as control. The right-side kidneys of animals in second group were treated with 2,000 18-kV shock waves under anesthesia. Localization of the right kidney was achieved following contrast medium injection through a tail vein under flouroscopy control. The animals were sacrificed 72 h after the ESWL treatment, and the kidneys were taken. Malondialdehyde level was higher in the ESWL group than in the control. Reduced glutathione levels, superoxide dismutase, and glutathione peroxidase activities were lower in the ESWL group than those of the control. Fe, Cu, Pb, Mn, Cd, and Ni levels were lower in the ESWL group than in the control, although Mg level was higher in the ESWL group than in the control. In conclusion, the result of the present study indicated that ESWL treatment produced oxidative stress in the kidney and caused impairments on the antioxidant and trace element levels in the kidneys of rats.

Effect of ageing and oxidative stress on antioxidant enzyme activity in different regions of the rat kidney

“…This observed reduction in the activity of antioxidant enzymes under stress conditions is in agreement with earlier reports using a range of oxidative and other challenges [1,39,40,41], The increased production of ROS that results from oxidative stress leads to modifications in lipids, carbohydrates, proteins and nucleic acids, resulting in cytotoxicity and dysfunction in several tissues [42].…”

Induction of Oxidative Stress in Kidney

“…In our work, we showed increased expression of inducible NO synthase (iNOS) and NF κ B, indirect evidence of increased OS [42]. Recently, Gecit et al showed that ESWL treatment produces OS and causes impairment in the antioxidant and trace element levels in the kidneys of rats [43]. ESWL is associated with greater prevalence of hypertension [44].…”

Effect of crush and axotomy of phrenic nerves on oxidative stress in diaphragm muscle of rats

“…SOD catalyzes the dismutation of the superoxide anion radical to water and hydrogen peroxide, which is detoxified by the CAT activity. Usually, a simultaneous induction response in the activities of SOD and CAT is observed when an exogenous antioxidant is applied 20, 21. Decreased tissue GSH‐Px activity in this study indicated that oxidation of GSH to GSSG is likely to be lowered by crush or axotomy of phrenic nerves.…”