Effects of short light-dark regimes on in vitro shoot rooting of some fruit tree rootstocks

Morini, S.; Perrone, S.

doi:10.1007/s10535-006-0062-9

Cited by 3 publications

(2 citation statements)

References 18 publications

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“…5b). MS medium is generally used for micropropagation, tissue regeneration and in vitro rooting of Prunus species (Morini and Perrone 2006;Kalinina and Brown 2007;Petri and Scorza 2010;Zhou et al 2010;Wang et al 2011;PerezJimenez et al 2012). Similar to our observation, IBA has also been suggested for root induction in some almond cultivars by Caboni et al (1997) and apricot cultivars by Koubouris and Vasilakakis (2006).…”

Section: In Vitro Rootingmentioning

confidence: 99%

Adventitious shoot regeneration from in vitro cultured leaf explants of peach rootstock Guardian® is significantly enhanced by silver thiosulfate

Şan

et al. 2014

Plant Cell Tiss Organ Cult

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Establishment of a stable and efficient in vitro regeneration system from leaf explants is a basic requirement for success in genetic transformation of peach. In this study, approximately 900 leaf explants of 'Guardian Ò ' peach genotype were separated from in vitro shoots and cultured for shoot regeneration on MS medium with different doses and combinations of thidiazuron (TDZ) and silver thiosulphate (STS). The results showed that callus bud formation and shoot regeneration occurred 4 and 6 weeks after culture initiation, respectively. All the regenerated shoots were from petioles of the leaf explants. Regeneration rates were significantly affected by different combinations of TDZ and STS (P \ 0.05). Separation of means by Tukey test method revealed that on half strength MS media, high regeneration rates were obtained from the combinations of 6.81 lM TDZ ? 10 lM STS, and of 9.08 lM TDZ ? 10 lM STS (16 and 14 %, respectively). The number of shoots per regenerating explants was also significantly affected by growth regulator concentrations. The highest shoot number was obtained with the combination of 6.81 lM TDZ ? 10 lM STS. Rooting percentage varied from 85 to 100 % depending on plant growth regulator used. On half strength MS medium containing 1.0 mg/l of IBA, an average of 7 roots per regenerating shoot was produced. The rooted plantlets were successfully acclimatized with 92 % survival rate under greenhouse conditions. This established regeneration system from leaf explants would facilitate the development of reliable peach genetic transformation procedures, helping accelerate peach genetic improvement using biotechnology approaches.

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Section: In Vitro Rootingmentioning

confidence: 99%

Adventitious shoot regeneration from in vitro cultured leaf explants of peach rootstock Guardian® is significantly enhanced by silver thiosulfate

Şan

et al. 2014

Plant Cell Tiss Organ Cult

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“…It can be easily multiplied through in vitro shoot tip culture (Morini and Perrone 2006). Mother shoot cultures used for the present trials were supplied by a commercial laboratory, and maintained (subcultures at 4 weeks) on a proliferation medium (MRS-P) with the following composition: Murashige and Skoog (1962) macro and microelements, and (μM): 555 myo-inositol, 2.96 thiamine hydrochloride, 8.1 nicotinic acid, 4.9 pyridoxine hydrochloride, 26.6 glycine, 2.2 6-benzyladenine (BA), and (w/v) 3% sucrose and 0.65% 'type A' agar; the pH was adjusted to 5.6-5.7 by the addition of 0.1-1 N KOH before autoclaving at 120°C (110 kPa) for 20 min.…”

Section: Plant Materialsmentioning

confidence: 99%

Elimination of in vitro bacterial contaminants in shoot cultures of ‘MRS 2/5’ plum hybrid by the use of Melia azedarach extracts

Marino¹,

Gaggìa²,

Saiano³

et al. 2008

Eur J Plant Pathol

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The antimicrobial activity of leaf and callus extracts of Melia azedarach was tested on in vitro shoot cultures of the peach rootstoch 'MRS 2/5' (Prunus cerasifera × Prunus spinosa) that were heavily contaminated with Sphingomonas paucimobilis (Sp) and Bacillus circulans (Bc). The extracts were filter-sterilised and added at 0%, 1%, 5%, 10% and 20% to a modified Murashige and Skoog proliferation medium previously autoclave-sterilised. Up to about 17% shoots died with 10-20% extract, except for Spcontaminated shoots, whose survival was reduced to 50% after treatment with 20% extract. No shoots died with 1% to 5% supplement. The undiluted leaf extract showed bactericidal activity on plated Sp and Bc isolates. The homogenates of shoots randomly collected from treated cultures were processed for bacterial colony counting. Thus the 10% supplement was the best treatment for ridding Bc-contaminated cultures of bacteria (although 5% had a similar bactericidal effect), and allowing shoot growth and proliferation comparable to controls at the fifth subculture on a standard medium, while 20% extract was needed to eliminate Sp, and could induce higher growth and proliferation rates in surviving shoots than in untreated cultures. Callus extract was ineffective. The bactericidal activity of the leaf extract seemed attributable to a synergistic effect of azadirachtin with other unidentified compounds present in the extract.

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Micropropagation of GF 677 Rootstock

Aghaye¹,

Yadollahi²

2012

JAS

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The aim of this investigation was to study micropropagation of Almond GF rootstock on in vitro. Explants were cultured in MS medium containing 5 concentrations of 6-Benzylaminoporine (BAP) 0.1, 0.6, 1, 1.5 and 2 mg 1 -1 along with control treatment. Each treatment includes 5 replications. After 5 weeks of culture, the results showed that the number and length of shoots in treatment containing 1 mg 1 -1 BAP was more than the other treatments and control. The number of shoot that was obtained in treatment of 1 mg 1 -1 BAP was 2.98 (Table 1). The lowest number of shoot was 0.4 due to control treatment. For rooting, explants with 3-5 cm length were transferred to different rooting treatments ( Table 2). The results revealed that the best rooting was obtained with treatment 3 mg 1 -1 IBA. Number and length of roots were 2.167 and 11.53, respectively. Also results showed that putting explants in dark for 1 week is more effective than light.

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Effects of short light-dark regimes on in vitro shoot rooting of some fruit tree rootstocks

Cited by 3 publications

References 18 publications

Adventitious shoot regeneration from in vitro cultured leaf explants of peach rootstock Guardian® is significantly enhanced by silver thiosulfate

Adventitious shoot regeneration from in vitro cultured leaf explants of peach rootstock Guardian® is significantly enhanced by silver thiosulfate

Elimination of in vitro bacterial contaminants in shoot cultures of ‘MRS 2/5’ plum hybrid by the use of Melia azedarach extracts

Micropropagation of GF 677 Rootstock

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