Effects of silver in isolated rat hepatocytes

Baldi, C.; Minoia, Claudio; Nucci, A. Di; Capodaglio, E; Manzo, Luigi

doi:10.1016/0378-4274(88)90063-x

Cited by 62 publications

(29 citation statements)

References 17 publications

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“…This precise delivery could lead to the effective control of wound bioburden without silver-related cytotoxicity currently encountered with macroscopic topical silver-delivery agents used for wound healing [5,15,38,40]. More broadly, our results using silver impregnated PEMs support the general concept of nanoscopic localization of bioactive molecules by nanometer thick polymer multilayers.…”

Section: Discussionsupporting

confidence: 67%

“…Cytotoxicity of silver to mammalian cells in vitro is well documented, with several researchers reporting on the cytotoxic effects of silver ions on fibroblasts [15,16], keratinocytes [15], hepatocytes [38], and lymphocytes [18]. In particular, Hidalgo et al [16] reported that silver nitrate concentrations greater than 2.8 ppm in growth media (supplemented with 10% fetal calf serum) were cytotoxic (greater than 90% cell death in 24 h) to human dermal fibroblasts (obtained from skin biopsies at their hospital).…”

Section: Resultsmentioning

confidence: 99%

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Surfaces modified with nanometer-thick silver-impregnated polymeric films that kill bacteria but support growth of mammalian cells

et al. 2010

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Silver is widely used as a biocidal agent in ointments and wound dressings. However, it has also been associated with tissue toxicity and impaired healing. In vitro characterization has also revealed that typical loadings of silver employed in ointments and dressings (∼ 100 μg/cm2) lead to cytotoxicity. In this paper, we report the results of an initial study that sought to determine if localization of carefully controlled loadings of silver nanoparticles within molecularly thin films immobilized on surfaces can lead to antimicrobial activity without inducing cytotoxicity. Polymeric thin films of poly(allylamine hydrochloride) (PAH) and poly(acrylic acid) (PAA) were prepared by layer-by-layer deposition and loaded with ∼0.4 μg/cm2 to ∼23.6 μg/cm2 of silver nanoparticles. Bacterial killing efficiencies of the silver-loaded films were investigated against Staphylococcus epidermidis, a gram-positive bacterium, and it was determined that as little as ∼0.4 μg/cm2 of silver in the polymeric films caused a reduction of 6 log10 CFU/mL (99.9999%) bacteria in suspensions incubated in contact with the films (water-borne assays). Significantly, whereas the antibacterial films containing high loadings of silver were found to be toxic to a murine fibroblast cell line (NIH-3T3), the polymeric films containing ∼0.4 μg/cm2 of silver were not toxic and allowed attachment, and growth of the mammalian cells. Thus, the results of this study go beyond prior reports by identifying silver-impregnated, polymeric thin films that are compatible with in vitro mammalian cell culture yet exhibit antibacterial activity. These results support the hypothesis that localization of carefully controlled loadings of silver nanoparticles within molecularly thin polymeric films can lead to antimicrobial activity without cytotoxicity. More broadly, this strategy of modifying surfaces with minimal loadings of bioactive molecules indicates the basis of approaches that may permit management of microbial burden in wound beds without impairment of wound healing.

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Section: Discussionsupporting

confidence: 67%

Section: Resultsmentioning

confidence: 99%

Surfaces modified with nanometer-thick silver-impregnated polymeric films that kill bacteria but support growth of mammalian cells

et al. 2010

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“…438,[496][497][498][499][500][501][502]502,503 Several systemic clinical side effects of silver may occur, such as argyrosis (eye), leucopenia, or liver and kidney damage. 420,430,496,504 For instance, histopathological analysis of liver tissue showed a dose-dependent toxicity of silver 494,505 and an inflammation with an influx of lymphocytes, but values can recover after aborting silver uptake. 438 Reports concerning the toxic effects of silver related to nerve tissue, centrally and in the periphery, are rather rare.…”

Section: Effects Of Silver Exposure On Tissues and Organsmentioning

confidence: 99%

Nanobio Silver: Its Interactions with Peptides and Bacteria, and Its Uses in Medicine

et al. 2013

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Introduction 4708 2. Peptides and Silver 4709 2.1. Silver Binding to Amino Acids and Peptides 4709 2.1.1. Silver Binding to Amino Acids − Theory 4709 2.1.2. Silver Binding to Amino Acids − Experiment 4711 2.1.3. Silver Binding to Peptides 4711 2.2. Natural Peptides Involved in Metal Detoxification 4714 2.3. Peptides for the Formation of Silver Nanostructures 4716 2.3.1. Biomineralization of Silver by Means of Peptides 4716 2.3.2. Peptides as Structure-Determining Scaffolds in the Synthesis of Silver Nanostructures 4721 3. Bacteria and Silver 4722 3.1. Antimicrobial Properties of Silver-Containing Compounds 4722 3.1.1. Interactions with the Bacterial Cell Wall 4722 3.1.2. Interactions with DNA, Enzymes, and Membrane Proteins 4724 3.1.3. Generation of Reactive Oxygen Species 4725 3.2. Bacterial Resistance Mechanisms against Ag(I) 4726 3.2.1. Accumulation and Storage-Based Mechanism 4726 3.2.2. Efflux Pump-Based Mechanism 4727 3.3. Synthesis of Silver Nanoparticles by Means of Bacteria 4728 4. Silver-Based Biomaterials in the Medical Field 4730 4.1. Types of Silver-Containing Biomaterials 4731 4.1.1. Metallic Silver Coatings and Silver's Antimicrobial Efficiency 4731 4.1.2. Silver-Containing Nanocomposites 4732 4.1.3. Silver-Containing Polymers 4732 4.1.4. Surface Modification with Ionic Silver Compounds 4734 4.1.5. Hybrid Silver Materials − Synergistic Effects 4734 4.2. Deposition Processes 4735 4.3. Mechanical Properties 4736 5. Biocompatibility of Silver 4737 5.1. General Routes of Silver Exposure 4737 5.2. Exposure to Silver-Containing Medical Products 4737 5.2.1. Dermal Contact: Burn Wound Dressings 4737 5.2.2. Bone Contact: Orthopedic and Dental Implants and Bone-Filling Products 4738 5.2.3. Blood Contact: Implants Introduced into the Vascular System 4739 5.3. Effects of Silver Exposure on Tissues and Organs 4739 5.3.1. Acute and Chronic Toxicity of Silver 4740 5.3.2. Tissue and Organ Damages 4740 5.3.3. Cellular Uptake of Silver 4741 5.4. Toxic Effects of Silver Nanoparticles 4741 5.5. Silver Detoxification 4742 5.5.1. Sequestration of Silver Ions 4742 5.5.2. Silver Ion Transport by ATPase P-type Efflux Pumps 4743 6. Conflicting Evidence 4744 7. Conclusions 4744 Author Information 4744 Corresponding Author 4744 Notes 4744 Biographies 4745 Acknowledgments 4746 References 4746

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“…It is slightly harder than gold and is very ductile and malleable [1]- [3]. Pure silver has the highest electrical and thermal conductivity of all metals and has the lowest contact resistance.…”

Section: Introductionmentioning

confidence: 99%

“…The subject of this investigation is prepared inorganic fluorides and complexes. The halo compounds have been utilized in bioinorganic chemistry as reagents for synthesizing some model compounds, such as AgF 3 . The unique properties of halide impart an unusual reactivity to the fluoride bonds which can be exploited in preparative inorganic chemistry or in catalysis.…”

Section: Introductionmentioning

confidence: 99%

Density Functional Theory Studies of Structural Properties and Natural Bond Orbital for a New Silver Halo Compound

Ghamami¹,

Lashgari²

2014

OALib

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In this theoretical study we used density functional theory to calculate the molecular structures of Silver Halo compound, AgF3. The molecular geometry, vibrational frequencies, energies and natural bond orbital (NBO) in the ground state are calculated by using the DFT (B3LYP) methods with LANL2DZ. The T.S guesses were generated by the linear synchronous transit method, at the DFT implemented on Gaussian 98 program. The geometries and normal modes of vibrations obtained from B3LYP calculations are in good agreement with the experimentally observed data.

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Effects of silver in isolated rat hepatocytes

Cited by 62 publications

References 17 publications

Surfaces modified with nanometer-thick silver-impregnated polymeric films that kill bacteria but support growth of mammalian cells

Surfaces modified with nanometer-thick silver-impregnated polymeric films that kill bacteria but support growth of mammalian cells

Nanobio Silver: Its Interactions with Peptides and Bacteria, and Its Uses in Medicine

Density Functional Theory Studies of Structural Properties and Natural Bond Orbital for a New Silver Halo Compound

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