Effects of single and multi walled carbon nanotubes on macrophages: Cyto and genotoxicity and electron microscopy

Giorgio, Maria Laura Di; Bucchianico, Sebastiano Di; Ragnelli, Anna Maria; Aimola, Pierpaolo; Santucci, S.; Poma, Anna

doi:10.1016/j.mrgentox.2011.02.008

Cited by 172 publications

(134 citation statements)

References 51 publications

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“…9 Many scholars who have examined the biological functions between carbon nanotubes (CNTs), graphene, fullerene, and cells have found that carbon nanoparticles can enter into cells or subcellular organelles, such as nuclei and lysosomes. [17][18][19][20][21]23,24 Carbon nanoparticles could induce apoptosis through oxidative stress. 33,34 As a type of critical target cell line for the study of liver cancer therapy, HepG2 has been investigated by many researchers.…”

Section: Introductionmentioning

confidence: 99%

Single-walled carbon nanohorn (SWNH) aggregates inhibited proliferation of human liver cell lines and promoted apoptosis, especially for hepatoma cell lines

Zhang

Sun

Bo³

et al. 2014

IJN

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Single-walled carbon nanohorns (SWNHs) may be useful as carriers for anticancer drugs due to their particular structure. However, the interactions between the material itself and cancerous or normal cells have seldom been studied. To address this problem, the effects of raw SWNH material on the biological functions of human liver cell lines were studied. Our results showed that unmodified SWNHs inhibited mitotic entry, growth, and proliferation of human liver cell lines and promoted their apoptosis, especially in hepatoma cell lines. Individual spherical SWNH particles were found inside the nuclei of human hepatoma HepG2 cells and the lysosomes of normal human liver L02 cells, implying that SWNH particles could penetrate into human liver cells_and the different interacted mechanisms on human normal cell lines compared to hepatoma cell lines. Further research on the mechanisms and application in treatment of hepatocellular carcinoma with SWNHs is needed.

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Section: Introductionmentioning

confidence: 99%

Single-walled carbon nanohorn (SWNH) aggregates inhibited proliferation of human liver cell lines and promoted apoptosis, especially for hepatoma cell lines

Zhang

Sun

Bo³

et al. 2014

IJN

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“…84,96 Quanto aos tratamentos químicos utilizados para a purificação há poucas informações descritas nos trabalhos. 84,93,96,125,126 Alguns destes utilizam amostras comerciais e não descrevem se o material foi ou não purificado ou quais ácidos foram usados para purificação. Essa informação é relevante, pois tratamentos com fortes oxidantes (p.ex.…”

Section: Considerações Finaisunclassified

Citotoxicidade e genotoxicidade de nanotubos de carbon

Franchi¹,

Santos²,

Matsubara³

et al. 2012

Quím. Nova

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Recebido em 2/5/11; aceito em 6/9/11; publicado na web em 8/11/11 CYTOTOXICITY AND GENOTOXICITY OF CARBON NANOTUBES. There are many controversies regarding the cyto-and genotoxicity of carbon nanotubes (CNTs). In this work, we discuss that many of the incongruous arguments are probably associated with the poor physical-chemical characterization of the CNT samples used in many publications. This manuscript presents examples of carbon nanostructures observed under high resolution electron microscopy that can be found in typical CNT samples, and shows which roles in cyto-and genotoxicity need to be better investigated. Issues concerning chemical treatment are addressed and examples of misunderstandings that can occur during the studies of cyto-and genotoxicity of CNT samples are given.Keywords: cell viability; DNA damage; carbon nanotube samples. INTRODUÇÃOA ideia de nanotecnologia como uma ciência de manipulação molecular ou de escala nanométrica sempre foi um fascínio para muitos pesquisadores. Feynman em 1959 e Drexler em 1986 já chamavam a atenção da comunidade científica com relação à nanotecnologia. 1,2 Com os avanços da microscopia e de outras técnicas, a nanotecnologia se tornou nos últimos 10 anos uma das áreas mais importantes da ciência e da inovação tecnológica com projeções comerciais estimadas para 2015 em um trilhão de dólares. 3,4 Nesse contexto a Nanotoxicologia desempenha um papel importante, pois investiga os efeitos dos nanodispositivos e nanoestruturas em sistemas biológicos. 5 A avaliação toxicológica de partículas e fibras em escala nanométrica 6 contribui para uma série de questões ligadas à utilização de nanomateriais em produtos comerciais. Sendo assim, o entendimento dos mecanismos de cito-e genotoxicidade de um dado nanomaterial é fundamental para a definição de seu impacto ambiental e de estratégias de proteção aos trabalhadores e consumidores. 7 O presente artigo apresenta uma revisão de estudos realizados sobre a eventual indução de danos às células e ao DNA (citotoxicidade e genotoxicidade, respectivamente) por nanotubos de carbono (NTCs), conduzidos em experimentos in vitro. Na literatura existente é possível detectar várias controvérsias e questões que ainda precisam ser esclarecidas já que algumas dessas nanoestruturas se apresentam biocompatíveis. Recentes estudos demonstraram, por exemplo, que a enzima mieloperoxidase presente em neutrófilos humanos é capaz de degradar nanotubos de carbono de parede simples (NTCPS) e que os produtos da biodegradação dos NTCs não induziram inflamação quando expostos aos pulmões de camundongo. [8][9][10][11][12] Embora haja um conflito sobre o mérito do descobrimento dos NTCs, 13 o fato é que esses são um dos nanomateriais mais fascinantes e estudados nas últimas duas décadas. Um NTCPS pode ser descrito basicamente como uma folha de grafeno enrolada e fechada em cada extremidade por metade de um fulereno. Quando várias folhas de grafeno constituem a parede do tubo os mesmos são classificados como NTCs de parede múltiplas (NTCPM). 14 Os NTCs aprese...

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“…According to various researchers, upon addition of CNTs to cells, CNTs could enter the cells and be toxic to their functions. 74 This would have negative implications for the genetic makeup of the cell. Therefore, further research needs to be done in order to reach definitive conclusions.…”

Section: Untreated III Pbs + Nir Iv Peg-swntsmentioning

confidence: 99%

“…Also, 24 hours after addition of CNTs to the RAW 264.7 cells, transmission electron microscopy indicated more phagocytic activity in comparison with normal cells, as shown by changes in nucleus morphology. 74 However , as mentioned earlier, the functionalization technique used will dramatically attenuate the toxicity of CNTs. 72 Morphology of the particles also plays a major role in their toxicity.…”

Section: Untreated III Pbs + Nir Iv Peg-swntsmentioning

confidence: 99%

“…Due to the size of the CNTs, these particles can easily become airborne and inhaled. 74 It has been suggested that widespread distribution of CNTs in the respiratory system could lead to symptoms similar to those that develop after exposure to asbestos. 77 Inhalation of asbestos fibers is known to induce chronic inflammation, scarring of the lungs (asbestosis), and malignant mesothelioma.…”

Section: Untreated III Pbs + Nir Iv Peg-swntsmentioning

confidence: 99%

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A new era of cancer treatment: carbon nanotubes as drug delivery tools

Seifalian¹

2011

IJN

123

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Cancer is a generic term that encompasses a group of diseases characterized by an uncontrolled proliferation of cells. There are over 200 different types of cancer, each of which gains its nomenclature according to the type of tissue the cell originates in. Many patients who succumb to cancer do not die as a result of the primary tumor, but because of the systemic effects of metastases on other regions away from the original site. One of the aims of cancer therapy is to prevent the metastatic process as early as possible. There are currently many therapies in clinical use, and recent advances in biotechnology lend credence to the potential of nanotechnology in the fight against cancer. Nanomaterials such as carbon nanotubes (CNTs), quantum dots, and dendrimers have unique properties that can be exploited for diagnostic purposes, thermal ablation, and drug delivery in cancer. CNTs are tubular materials with nanometer-sized diameters and axial symmetry, giving them unique properties that can be exploited in the diagnosis and treatment of cancer. In addition, CNTs have the potential to deliver drugs directly to targeted cells and tissues. Alongside the rapid advances in the development of nanotechnology-based materials, elucidating the toxicity of nanoparticles is also imperative. Hence, in this review, we seek to explore the biomedical applications of CNTs, with particular emphasis on their use as therapeutic platforms in oncology.

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Effects of single and multi walled carbon nanotubes on macrophages: Cyto and genotoxicity and electron microscopy

Cited by 172 publications

References 51 publications

Single-walled carbon nanohorn (SWNH) aggregates inhibited proliferation of human liver cell lines and promoted apoptosis, especially for hepatoma cell lines

Single-walled carbon nanohorn (SWNH) aggregates inhibited proliferation of human liver cell lines and promoted apoptosis, especially for hepatoma cell lines

Citotoxicidade e genotoxicidade de nanotubos de carbon

A new era of cancer treatment: carbon nanotubes as drug delivery tools

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