Effects of sodium butyrate and dimethylsulfoxide on human pancreatic tumor cell lines

McIntyre, Laurence J.; Kim, Young S.

doi:10.1016/0277-5379(84)90194-9

Cited by 25 publications

(15 citation statements)

References 13 publications

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“…Specifically, Capan-1 bound more avidly to type I collagen compared with MIA PaCa-2 10, 25–28. Additionally, BxPC-3 and PANC-1 appeared to bind with equivalent affinity to type I collagen 25, 29, 30.…”

Section: Adhesionmentioning

confidence: 92%

“…In athymic mice, Capan-1 derived tumor produced mucin and was morphologically and biochemically similar to the tumor of origin 9. Although not reported in the original publication, a doubling time of 41 hours was subsequently determined for Capan-110.…”

Section: Clinical Picture and Cell Line Derivationmentioning

confidence: 99%

“…Subsequent studies report substantial differences from the original publications. For example, McIntyre and Kim report doubling times of 26 hours for MIA PaCa-2 and 28 hours for PANC-1, whereas the original doubling times were reported as 40 and 52 hours respectively 10.…”

Section: Clinical Picture and Cell Line Derivationmentioning

confidence: 99%

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Phenotype and Genotype of Pancreatic Cancer Cell Lines

et al. 2010

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The dismal prognosis of pancreatic adenocarcinoma (PA) is due in part due to a lack of molecular information regarding disease development. Established cell lines remain a useful tool for investigating these molecular events. Here we present a review of available information on commonly used PA cell lines as a resource to help investigators select the cell lines most appropriate for their particular research needs. Information on clinical history, in vitro and in vivo growth characteristics, phenotypic characteristics, such as adhesion, invasion, migration and tumorigenesis, and genotypic status of commonly altered genes (KRAS, p53, p16, and SMAD4) was evaluated. Identification of both consensus and discrepant information in the literature suggests careful evaluation before selection of cell lines and attention be given to cell line authentication.

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Section: Adhesionmentioning

confidence: 92%

Section: Clinical Picture and Cell Line Derivationmentioning

confidence: 99%

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Phenotype and Genotype of Pancreatic Cancer Cell Lines

et al. 2010

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“…For example, reports for the doubling time of PANC-1 cells include 52 h [56], 38 h [57], and 28 h [58], which may also reflect heterogeneity. Over 96 h of culture, we observed a progressive change in cell cycle distribution for the unperturbed control group, which consisted of a small, time-dependent accumulation of cells in the G1 phase.…”

Section: Discussionmentioning

confidence: 99%

Mechanism-based mathematical modeling of combined gemcitabine and birinapant in pancreatic cancer cells

Zhu

Straubinger

Jusko

2015

J Pharmacokinet Pharmacodyn

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Combination chemotherapy is standard treatment for pancreatic cancer. However, current drugs lack efficacy for most patients and selection and evaluation of new combination regimens is empirical and time-consuming. The efficacy of gemcitabine, a standard-of-care agent, combined with birinapant, a pro-apoptotic antagonist of Inhibitor of Apoptosis Proteins (IAPs), was investigated in pancreatic cancer cells. PANC-1 cells were treated with vehicle, gemcitabine (6, 10, 20 nM), birinapant (50, 200, 500 nM), and combinations of the two drugs. Temporal changes in cell numbers, cell cycle distribution, and apoptosis were measured. A basic pharmacodynamic (PD) model based on cell numbers, and a mechanism-based PD model integrating all measurements, were developed. The basic PD model indicated that synergistic effects occurred in both cell proliferation and death processes. The mechanism-based model captured key features of drug action: temporary cell cycle arrest in S phase induced by gemcitabine alone, apoptosis induced by birinapant alone, and prolonged cell cycle arrest and enhanced apoptosis induced by the combination. A drug interaction term ψ was employed in the models to signify interactions of the combination when data were limited. When more experimental information was utilized, ψ values approaching 1 indicated that specific mechanisms of interactions were captured better. PD modeling identified the potential benefit of combining gemcitabine and birinapant, and characterized the key interaction pathways. An optimal treatment schedule of pretreatment with gemcitabine for 24-48 h was suggested based on model prediction and was verified experimentally. This approach provides a generalizable modeling platform for exploring combinations of cytostatic and cytotoxic agents in cancer cell cultures.

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“…DMSO has been shown to reduce growth of a human ovarian carcinoma (HOC-7) cell line reversibly in a dose-and time dependent manner 20 and to reduce the tumourigenicity of human pancreatic cell lines. 21 Conversely, few papers exist that claim to use ethanol as a gelating medium. A rare, recent example uses a gel formulated with 40 % (v/v) ethanol to demonstrate the enhanced release profile of indomethacin.…”

Section: Introductionmentioning

confidence: 99%