Effects of Some Parameters on Particle Size Distribution of Chitosan Nanoparticles Prepared by Ionic Gelation Method

Vaezifar, Sedigheh; Razavi, Shahnaz; Golozar, M.A.; Karbasi, Saeed; Morshed, Mohammad; Kamali, Mohammad

doi:10.1007/s10876-013-0583-2

Cited by 138 publications

(73 citation statements)

References 30 publications

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“…In both of them the band at 3436 cm -1 was assigned to NH 2 and OH stretching vibrations. The bands at 1657 cm -1 , 1598 cm -1 and 1320 cm -1 were attributed to Amide I, -NH 2 bending and Amide III, respectively (Vaezifar et al, 2013;Zhang et al, 2012). The only difference was observed at 1155 cm -1 in chitosan nanoparticles.…”

Section: Analysis Of Ft-ir Spectramentioning

confidence: 88%

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Stability Improvement of Immobilized Alkaline Phosphatase Using Chitosan Nanoparticles

Jafary

Panjehpour

Varshosaz

et al. 2016

Braz. J. Chem. Eng.

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-Enzyme engineering via immobilization techniques is a suitable approach for improving enzyme function and stability and is superior to the other chemical or biological methods. In this study chitosan nanoparticles were synthesized using the Ionic Gelation method and were characterized by Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM). Alkaline phosphatase was successfully immobilized on the chitosan nanoparticles in optimum conditions. Chitosan nanoparticles were used because of their special properties for enzyme immobilization. This study indicated that the immobilized enzyme has improved function at high temperature and during storage. Immobilization resulted in an increased range of optimum pH and temperature, and reusability of enzyme. Furthermore, the binding efficiency calculation indicated that the immobilized alkaline phosphatase conserved 71% of its native activity. Kinetic parameter studies indicated no significant difference between the immobilized and free enzymes.

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Section: Analysis Of Ft-ir Spectramentioning

confidence: 88%

“…Preparation of chitosan nanoparticles was performed according to the procedure of Vaezifar et al (2013). Chitosan powder (5 mg) was dissolved in 1.0% (w/v) acetic acid.…”

Section: Preparation Of Chitosan Nanoparticlesmentioning

confidence: 99%

Stability Improvement of Immobilized Alkaline Phosphatase Using Chitosan Nanoparticles

Jafary

Panjehpour

Varshosaz

et al. 2016

Braz. J. Chem. Eng.

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“…Pentasodium tripolyphosphate (TPP) anions were used to prepare chitosan nanoparticles according to the ionotropic gelation of chitosan . Chitosan powder (Sigma‐Aldrich) was dissolved in 1% acetic acid aqueous solutions.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the preventive and therapeutic effects of a recombinant vector co‐expressing prostate‐specific stem cell antigen and Clostridium perfringens enterotoxin on prostate cancer in rats

Abedi

Doosti

Jami

2019

Biotechnology Progress

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The effects of Clostridium perfringens enterotoxin (CPE) and prostate stem cell antigen (PSCA) on cancer prevention or treatment have been previously studied separately. For the first time, here we have elaborated a recombinant vector to co‐express and study the cumulative effects of both of these factors on prostate cancer (PCa) in an animal model. The recombinant pBudCE4.1‐cpe‐PSCA vector was constructed in large scale. Rats were vaccinated by vector or vector plus chitosan nanoparticles before or after induction of PCa (preventive or therapeutic studies) by N‐methyl N‐nitrosurea and testosterone. Prostate tumors were weighed and histologically examined. Tumors and infusion site tissues as well as blood samples of all rats were collected and assessed by serological and molecular tests. We showed that vaccination with vector (along with or without nanoparticles) led to lower PCa incidence and tumor weight. The L‐1β, IL6, and TNF‐α serum levels and their gene expression accompanied by C‐CAM1 gene expression in vaccinated groups were significantly higher than controls while no difference was seen in CK20 expression among all groups. Our findings showed that vector could effectively stimulate the immune system of rats to either prevent or suppress the PCa tumors. Adding chitosan nanoparticles did not affect the results significantly.

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“…The decreasing of crystallinity could be due to that chitosan nanoparticles are composed of a dense network structure of interpenetrating counter ions TPP, where the polymer chains crosslink with each other by TPP. Thus the XRD pattern of chitosan nanoparticles is characteristic of an amorphous polymer [20]. The surface and stability of the prepared chitosan nanoparticles was characterized using zeta potential, it was found that the surface was shown to be positively charged (+38 mv).…”

Section: Removal Of Fe (Ii) and Mn (Ii) From Wastewater …mentioning

confidence: 99%

“…Chitosan nanoparticles were formed spontaneously upon addition of 3 ml chitosan solution under vigorous magnetic stirring to 1 ml of an aqueous TPP solution (0.25% w/v), with a ratio of chitosan to TPP 3:1 at room temperature. Nanoparticles were separated by centrifugation at 9000×g for 20 min [20]. Chitosan nanoparticles were extensively rinsed with DI to remove any NaOH residues and dried at 40C o for 24 h before further use or analysis [21].…”

Section: Preparation Of Chitosan Nanoparticlesmentioning

confidence: 99%

Removal of Fe (Ii) and Mn (Ii) From Wastewater Using Nano-Chitosan Prepared From Shrimp Waste

Elkady

Fathallah

Elfadl

et al. 2017

Al-Azhar Bulletin of Science

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Effects of Some Parameters on Particle Size Distribution of Chitosan Nanoparticles Prepared by Ionic Gelation Method

Cited by 138 publications

References 30 publications

Stability Improvement of Immobilized Alkaline Phosphatase Using Chitosan Nanoparticles

Stability Improvement of Immobilized Alkaline Phosphatase Using Chitosan Nanoparticles

Evaluation of the preventive and therapeutic effects of a recombinant vector co‐expressing prostate‐specific stem cell antigen and Clostridium perfringens enterotoxin on prostate cancer in rats

Removal of Fe (Ii) and Mn (Ii) From Wastewater Using Nano-Chitosan Prepared From Shrimp Waste

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