Effects of Source Leukocyte Collection on the
Immune System

Orsini, F.; Fitzpatrick, J.; Cohen, E.; Khurana, U.; Michielson, C.; Chadha, K.C.; Han, T.; Ozer, H.

doi:10.1159/000465349

Cited by 4 publications

(6 citation statements)

References 10 publications

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“…The recent report by Orsini et al [7] indicating that donors who were subjected to frequent double bag leukapheresis failed to show significant changes in a number of immune parameters is consistent with our findings. The impor tance of our studies relates to unavoidable but significant lymphocyte losses that occur during repeat cytapheresis of normal blood donors and suggests that the immune system of normal subjects may not be adversely affected by the loss of as many as 40 x 109 peripheral blood lymphocytes, at least when such losses occur over a relatively short pe riod of time.…”

Section: Discussionsupporting

confidence: 93%

“…Concern has been expressed about potential adverse affects of such lymphocyte losses [6,7], but little is known about either the short or long-term effects and sequelae of lymphocyte depletion on immune respon siveness of healthy individuals. This lack of information prompted us to study the effect, and thus potential safety, of repeated short term removal of lymphocytes from healthy blood donors.…”

mentioning

confidence: 99%

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Immune Function in Blood Donors Following Short-Term Lymphocytapheresis

Blanchette¹,

Dunne²,

Steele³

et al. 1985

Vox Sanguinis

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Lymphocyte losses occur during repeat plateletpheresis and/or leukapheresis procedures and are potentially harmful to normal host immune function. This concern prompted us to study the effect of repeat lymphocytapheresis (lymphapheresis) on the immune system of healthy blood donors. Following a total of six lymphapheresis procedures over a 12-day period with removal of a mean of 41.6 x 10^9 lymphocytes, there were no significant changes in donor (n = 5) absolute lymphocyte counts, quantitative immunoglobulin levels or immune response capability as assessed by the following in vitro assays: percent of E, EA, and EAC rosette-forming cells, the percent of surface membrane immunoglobulin bearing cells, and the blastogénie responses to stimulation with phytohemagglutinin, pokeweed mitogen and concanavalin A. The procedures were well-tolerated without complications. During a follow-up period of 18 months, significant increases in total white cell counts (but not absolute lymphocyte counts), and in quantitative immunoglobulin levels (IgG, IgM, and IgA) were observed. The median increases in immunoglobulin levels ranged from 27.5% for IgG to 44% for IgA. We conclude that lymphapheresis as described is relatively safe and our results are reassuring in relation to lymphocyte losses that may occur during repeat cytapheresis procedures of normal blood donors.

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Section: Discussionsupporting

confidence: 93%

mentioning

confidence: 99%

Immune Function in Blood Donors Following Short-Term Lymphocytapheresis

Blanchette¹,

Dunne²,

Steele³

et al. 1985

Vox Sanguinis

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“…These studies investigated the possible role of complement activation (11), lymphocyte and monocyte function (12) and immunological changes (13). The potential role of granulocytes, however, was not taken into consideration.…”

Section: Discussionmentioning

confidence: 99%

“…Investigations of complement activation (11) and functional analysis of monocytes and lymphocytes during cytapheresis have been reported (12,13). Similar studies with granulocytes, however, remain to be performed.…”

mentioning

confidence: 97%

On the Biocompatibility of IBM 2997 Continuous Flow Plateletapheresis

Lindena¹,

Burkhardt²,

Aulmann³

1990

Clinical Chemistry and Laboratory Medicine

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Summary:During the procedure of centrifugation cytapheresis donors occasionally experience adverse clincial reactions. We evaluated the possibility of whether activation of granulocytes and their subsequent release reactions, which may have been triggered by this extracorporeal circuit, were responsible for these adverse effects. Six blood samples were obtained during various set intervals during plateletapheresis. Of these, four samples were taken directly from each donor. The remaining two were drawn from the efferent lines, i.e. those which return blood from the cytapheresis machine back to the donor. Reactive oxygen species produced by granulocytes were monitored by chemiluminescence using microamounts of whole blood or isolated granulocytes. Furthermore, secreted granulocyte products such as neutral proteinase elastase, present in plasma in a complex with oti-proteinase inhibitor (complexed elastase), and lysosomal -glucuronidase were examined. A complete blood cell count, as well as values of haemoglobin, haematocrit, lactate dehydrogenase, protein, albumin and proteinase inhibitors such as oc 2 -macroglobulin and αϊ-proteinase inhibitor were also determined. Complexed elastase increased from a preapheresis value of about 140 μg/l to about 180 μg/l at the end of the cytapheresis. All other clinical chemical and cytological values were 8 to 12 percent lower than preapheresis values, which can be attributed to inherent plasma volume expansion. Reduced chemiluminescence was observed upon stimulation of phagocytes in the whole blood assay (about 700 counts/min χ ΙΟ 3 χ 50000 cells vs. about 600 counts/min χ ΙΟ 3 χ 50000 cells). This decrease was also seen with stimulated granulocytes (about 5800 counts/min χ ΙΟ 3 χ 50000 cells vs. about 4500 counts/min χ ΙΟ 3 χ 50000 cells). Unstimulated granulocytes, on the other hand, showed an increased native chemiluminescence. These data do not, however, indicate a cytapheresis mediated activation of the oxidative metabolism of granulocytes. Concomitant discharge of proteolytic enzymes remains, therefore, of no clinical importance.

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“…These investigations have been addressed to the possible role of complement activation," lymphocyte and nionocyte function," and immunologic changes. 13 The poten-…”

Section: Discussionmentioning

confidence: 99%

Evaluation of granulocyte‐releasing products and chemiluminescence during cytapheresis

Lindena¹,

Sommerfeld²,

Burkhardt³

et al. 1989

Transfusion

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During the procedures of centrifugation leukapheresis and plateletpheresis, donors occasionally experience adverse clinical reactions. The possibility of whether the activation of granulocytes and the subsequent release reactions, which may have been triggered by this extracorporeal circuit, were responsible for these adverse effects was evaluated. Six blood samples were obtained at set intervals during cytapheresis. Of these samples, four were taken directly from the donor. The remaining two were drawn from the efferent lines, i.e., those which return blood from the cytapheresis machine to the donor. Reactive oxygen species produced by granulocytes were measured by chemiluminescence (CL) using microamounts of whole blood or isolated granulocytes. Furthermore, secreted granulocyte products such as neutral proteinase elastase, which is present in plasma in a complex with alpha-1-proteinase inhibitor (E-alpha-1-PI), and lysosomal beta-glucuronidase were examined. A complete blood cell count and the values of hemoglobin, hematocrit, lactate dehydrogenase, protein, albumin, and proteinase inhibitors such as alpha-2-macroglobulin and alpha-1-proteinase inhibitor were also determined. Clinical chemical and cytologic values, with the exception of those for E-alpha-1-PI, were 10 to 17 percent lower than values before apheresis. These results can be attributed to inherent plasma volume expansion. Reduced CL was observed on the stimulation of phagocytes in the whole blood assay, as well as with stimulated granulocytes. Unstimulated granulocytes, on the other hand, showed an increased native CL. These data do not indicate a cytapheresis-mediated activation of the oxidative metabolism of granulocytes, and the concomitant discharge of proteolytic enzymes remains, therefore, of no clinical importance.

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Effects of Source Leukocyte Collection on the Immune System

Cited by 4 publications

References 10 publications

Immune Function in Blood Donors Following Short-Term Lymphocytapheresis

Immune Function in Blood Donors Following Short-Term Lymphocytapheresis

On the Biocompatibility of IBM 2997 Continuous Flow Plateletapheresis

Evaluation of granulocyte‐releasing products and chemiluminescence during cytapheresis

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