Effects of Soybean .BETA.-Conglycinin on Body Fat Ratio and Serum Lipid Levels in Healthy Volunteers of Female University Students

Baba, Toshimitsu; Ueda, Aiko; Kohno, M.; Fukui, Kensuke; Miyazaki, Chiaki; Hirotsuka, Motohiko; Ishinaga, Masataka

doi:10.3177/jnsv.50.26

Cited by 20 publications

(12 citation statements)

References 8 publications

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“…For example, it inhibits arteriosclerosis, decreases the serum triglyceride levels as well as appetite in mouse (Adams et al, 2004;Moriyama et al, 2004;Nishi et al, 2003). In a study performed with 41 healthy female university students, Baba et al (2004) observed that when β-conglycinin was ingested at 5 g a day, the body fat ratio was maiteined and excessive serum lipid levels were eliminated. Therefore, we should try to control the protein level both up and down depending on the end-user demand.…”

Section: Discussionmentioning

confidence: 96%

Molecular characterization of a β-conglycinin deficient soybean

2006

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The soybean seed storage protein β-conglycinin has a low amino acid score, shows lower functional gelling properties compared with glycinin and contains a major allergen. The wild soybean (Glycine soja Sieb et Zucc.) QT2 lacks all the subunits of β-conglycinin, and this deficiency is controlled by a single dominant gene Scg-1 (suppressor of β-conglycinin). Scg-1 was introduced into a soybean cultivar Fukuyutaka from QT2 and this near-isogenic line was designated as QY7-25. Segregation analyses of the progeny derived from a cross between QY7-25 and the wild type did not show any significant changes caused by Scg-1 in the germination ratio and seed weight. Low amounts of mRNAs for the α , α and β subunits of β-conglycinin were detected by RT-PCR in QY7-25. We revealed that an α subunit mRNA expressed from a region which replaced with mutant line in the near-isogenic line QY7-25 by single nucleotide polymorphisms analysis. In addition, an abnormal splicing event in a cDNA clone for the β subunit isolated from immature seed of QY7-25 was observed. Southern analysis using the coding region of α subunit gene as a probe revealed a polymorphism between QY7-25 and wild type and this genotypes co-segregate with the deficiency of β-conglycinin subunits. These results suggest that the β-conglycinin deficiency might be controlled by a claster region of β-conglycinin subunit genes. In the present study, no agronomical disadvantage in QY7-25 was observed, confirming that Scg-1 is a valuable gene for soybean breeding.

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Section: Discussionmentioning

confidence: 96%

Molecular characterization of a β-conglycinin deficient soybean

2006

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“…Despite the negative factors associated with b-conglycinin, it has several health benefits. It was shown that b-conglycinin is effective in decreasing serum triglyceride levels (Moriyama et al 2004) and maintaining body fat ratios (Baba et al 2004). The peptide fragment VRIRLLQRFNKRS of the b subunit of b-conglycinin induced early satiety (Nishi et al 2003).…”

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confidence: 98%

The β-conglycinin deficiency in wild soybean is associated with the tail-to-tail inverted repeat of the α-subunit genes

Tsubokura

Hajika

Kanamori³

et al. 2011

Plant Mol Biol

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β-conglycinin, a major seed protein in soybean, is composed of α, α', and β subunits sharing a high homology among them. Despite its many health benefits, β-conglycinin has a lower amino acid score and lower functional gelling properties compared to glycinin, another major soybean seed protein. In addition, the α, α', and β subunits also contain major allergens. A wild soybean (Glycine soja Sieb et Zucc.) line, 'QT2', lacks all of the β-conglycinin subunits, and the deficiency is controlled by a single dominant gene, Scg-1 (Suppressor of β-conglycinin). This gene was characterized using a soybean cultivar 'Fukuyutaka', 'QY7-25', (its near-isogenic line carrying the Scg-1 gene), and the F₂ population derived from them. The physical map of the Scg-1 region covered by lambda phage genomic clones revealed that the two α-subunit genes, a β-subunit gene, and a pseudo α-subunit gene were closely organized. The two α-subunit genes were arranged in a tail-to-tail orientation, and the genes were separated by 197 bp in Scg-1 compared to 3.3 kb in the normal allele (scg-1). In addition, small RNA was detected in immature seeds of the mutants by northern blot analysis using an RNA probe of the α subunit. These results strongly suggest that β-conglycinin deficiency in QT2 is controlled by post-transcriptional gene silencing through the inverted repeat of the α subunits.

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“…Previously, it was reported by this laboratory that -conglycinin decreased plasma TG concentrations in young and adult rats 7) , and that -conglycinin reduced TG levels in patients with hyperlipidemia who consumed -conglycinin for 4 weeks in a small-scale clinical trial 8) . Moreover, -conglycinin consumption was shown to reduce or suppress increases in the body fat ratio in healthy female volunteers with a high baseline body fat ratio by the bioelectrical impedance method 9,10) . These reports suggested that -conglycinin may be a food ingredient that could be used to prevent and/ or ameliorate lifestyle-related diseases, such as hyperlipidemia and obesity.…”

Section: Introductionmentioning

confidence: 99%

Decreases in Serum Triacylglycerol and Visceral Fat Mediated by Dietary Soybean .BETA.-conglycinin

Kohno

Hirotsuka

Kito

et al. 2006

JAT

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129

112

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Soy protein isolate (SPI) is known to reduce the risk of heart disease by lowering serum cholesterol and triacylglycerol (TG) levels. Soybean -conglycinin, which is a component of SPI, might be the active ingredient that prevents and/or ameliorates lifestyle-related diseases, such as hyperlipidemia and obesity. This study aimed to determine the efficacy of soybean -conglycinin for lowering the human serum TG level and visceral fat. Randomized double-blind placebo-controlled designs were used to test the effect of dietary -conglycinin, which was taken in the form of candy.

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Effects of Soybean .BETA.-Conglycinin on Body Fat Ratio and Serum Lipid Levels in Healthy Volunteers of Female University Students

Abstract: The changes in body fat ratio and serum lipids induced by the ingestion of f3

Cited by 20 publications

References 8 publications

Molecular characterization of a β-conglycinin deficient soybean

Molecular characterization of a β-conglycinin deficient soybean

The β-conglycinin deficiency in wild soybean is associated with the tail-to-tail inverted repeat of the α-subunit genes

Decreases in Serum Triacylglycerol and Visceral Fat Mediated by Dietary Soybean .BETA.-conglycinin

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