Effects of Sperm Concentration and Straw Volume on Motion Characteristics and Plasma, Acrosomal, and Mitochondrial Membranes of Equine Cryopreserved Spermatozoa

Nascimento, Juliana; Raphael, Cláudia Fernandes; Andrade, André Furugen Cesar de; Alonso, Maria Augusta; Celeghini, Eneiva Carla Carvalho; Arruda, R. P.

doi:10.1016/j.jevs.2008.04.010

Cited by 45 publications

(31 citation statements)

References 36 publications

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“…The elucidation to these differences could be in differences among the species or in more technical ability to differentiate the stain nuances of MITO when bound to mitochondrial membranes with or without potential. Recently, Celeghini et al (2008) and Nascimento et al (2008) utilized the fluorescent probes association to evaluate the effects of the cryopreservation on bovine and equine sperm membranes, respectively. Graham et al (1990) associated the fluorescent probes to evaluate the bovine sperm, but they utilized a flow cytometry technique.…”

Section: Discussionmentioning

confidence: 99%

Damage assessment of the equine sperm membranes by fluorimetric technique

Celeghini

Andrade

Raphael

et al. 2010

Braz. arch. biol. technol.

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Section: Discussionmentioning

confidence: 99%

Damage assessment of the equine sperm membranes by fluorimetric technique

Celeghini

Andrade

Raphael

et al. 2010

Braz. arch. biol. technol.

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“…Ten microliters were placed in Makler Counting Chamber (Sefi Medical Instruments Ltd.), which was then inserted into the computer-assisted sperm analysis (CASA, Hamilton Thorne Biosciences equipment, HTM-IVOS, 12.3 version, Beverly, MA, USA). The animal motility software was previously adjusted for equine semen [16]. Rapid motility was defined as the percentage of motile spermatozoa with average path velocity (VAP) higher than 20µm/sec.…”

Section: Computer-assisted Sperm Analysis (Casa)mentioning

confidence: 99%

“…The protocol used to evaluate simultaneously plasma and acrosomal membrane integrity and mitochondrial membrane potential was based in Nascimento et al [16] and Celeghini et al [17]. Briefly, thawed semen was diluted in TALP sperm (25x10 6 spermatozoa/mL), and an 150-µL aliquot of this was placed in a pre-warmed micro centrifuge …”

Section: Membranes Analysismentioning

confidence: 99%

Addition of Pentoxifylline to Skim Milk–Based Extender on Frozen-Thawed Equine Sperm

Tsunoda

Arruda

Serrano-Recalde

et al. 2015

Journal of Equine Veterinary Science

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“…A associação de PI, H342, FITC-PSA e JC-1 é uma técnica validada e utilizada para avaliar sêmen de touros (GRAVANCE et al, 2000;CELEGHINI et al, 2007) suínos , carneiros (CELEGHINI et al, 2010b) e equinos (GRAVANCE et al, 2000;NASCIMENTO et al, 2008;CELEGHINI et al, 2010a). …”

Section: Potencial De Membrana Mitocondrialunclassified

“…The sperm movement was evaluated using the Computer-Assisted Sperm Analysis (CASA), as described by Nascimento et al (2008). Integrity of plasma and acrosomal membranes and mitochondrial membrane potential, using the fluorescent probes association Propidium Iodide (PI), Hoechst 33342 (H342), 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl carbocyanine iodide (JC-1) and fluorescein isothiocyanate-conjugated Pisum sativum agglutinin (FITC-PSA) adapted from Nascimento et al (2008) and Celeghini et al (2010a).…”

Section: Semen Analysismentioning

confidence: 99%

Efeitos da pentoxifilina sobre a qualidade espermática e hemodinâmica uterina em equinos

Tsunoda¹

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AGRADECIMENTOSÀ Deus, por fazer com que tudo sempre ocorra de forma melhor do que eu tinha planejado, por ter permitido que esse experimento ocorresse, o qual me trouxe tantas aprendizagens tanto para a vida profissional como para a pessoal;À minha família, obrigada por estarem sempre ao meu lado, me incentivarem e apoiarem na concretização de um sonho, que souberam compreender meus períodos de ausência;Ao Cássio, obrigada pelo companheirismo, incentivo diário, por torcer por mim, por me acalmar e me ajudar a enfrentar meus medos e minha ansiedade;À professora Eneiva Carla, obrigada pela oportunidade, pela amizade, por ter confiado em mim. Professora, você é um exemplo de ser humano para mim, honesta e humilde, com quem tive muito prazer em trabalhar;À Bruna Marcele Oliveira, obrigada pela amizade, pelo apoio desde que entrei para a equipe, obrigada por ter aceitado a aventura, pelo companheirismo em momentos difíceis, e pelas risadas que ficaram de tudo que passamos juntas;À minha ex-equipe, Andressa, Diego João, Rodrigo obrigada pelos ensinamentos, pela amizade, pelas risadas, pelas viagens de projetos, foi um prazer conhecê-los e conviver com cada um de vocês;Ao meu amigo querido Marcilio, quem não mediu esforços para me ajudar nesta Dissertação, obrigada pelos ensinamentos, pela paciência, pelos conselhos, por ter estado ao meu lado desde que cheguei à USP; À Mariana Rodrigues, obrigada pela amizade, pelo companheirismo, por me ouvir tantas vezes que precisei e por me ajudar a enxergar uma saída em dias tensos; À Carolina Rocha, obrigada por tudo que já fez por mim, pela amizade que construímos no decorrer deste mestrado, você faz muita falta no meu dia-dia. Pentoxifylline addition did not exert effect on progressive motility, however, pentoxifylline increased path velocity, progressive velocity and curvilinear velocity when compared to controls. There were no significant differences on the percentage of cells presenting intact plasma membrane, intact acrosome and high mitochondrial membrane potential (IPIAH). No statistical difference was found on uterine blood flow of mares after treatments. However, endometrial cytology showed a major inflammatory response on groups containingPentoxifylline when compared to controls. In conclusion, pentoxifylline exerted a positive effect on velocity parameters, but do not influence cellular membranes and chromatin.Furthermore, when mares were inseminated with semen containing pentoxifylline, an intense inflammatory response was observed.

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Effects of Sperm Concentration and Straw Volume on Motion Characteristics and Plasma, Acrosomal, and Mitochondrial Membranes of Equine Cryopreserved Spermatozoa

Cited by 45 publications

References 36 publications

Damage assessment of the equine sperm membranes by fluorimetric technique

Damage assessment of the equine sperm membranes by fluorimetric technique

Addition of Pentoxifylline to Skim Milk–Based Extender on Frozen-Thawed Equine Sperm

Efeitos da pentoxifilina sobre a qualidade espermática e hemodinâmica uterina em equinos

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