Effects of sperm insemination on the final meiotic maturation of mouse oocytes arrested at metaphase I after<i>in vitro</i>maturation

Yoon, Joonsung; Juhn, Kyoung Mi; Yoon, So Jeong; Ko, Yongmin; Lim, Jin Ho

doi:10.5653/cerm.2017.44.1.15

Cited by 8 publications

(4 citation statements)

References 26 publications

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“…The increased Ca 2+ oscillations trigger EDA in mouse and pig oocytes [40][41][42]. Our data show a moderate increase of cytosolic free Ca 2+ level in oocytes that underwent spontaneous EDA after 7 h of in vitro culture.…”

Section: Rosmentioning

confidence: 59%

Spontaneous Exit from Diplotene Arrest Associates with the Increase of Calcium and Reactive Oxygen Species in Rat Oocytes Cultured In Vitro

Tiwari

Chaube

2017

ROS

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Several signal molecules modulate the physiology of mammalian oocytes. Changes in the level of various signal molecules and their downstream impact on maturation promoting factor (MPF) during the achievement of meiotic competency remain ill understood. Therefore, the present study aimed to analyze levels of various signal molecules and MPF during the achievement of meiotic competency in rat oocytes. For this purpose, cumulus oocyte complexes (COCs) were collected from animals treated with 20 IU pregnant mare's serum gonadotropin (PMSG) for 48 h, followed by 20 IU human chorionic gonadotropin (hCG) for 14 h. The morphological changes, meiotic status and oocyte competency, cytosolic free calcium (Ca 2+), total reactive oxygen species (ROS), inducible nitric oxide synthase (iNOS), adenosine 3′,5′-cyclic monophosphate (cAMP), guanosine 3′,5′-cyclic monophosphate (cGMP), cell division cycle 25B (Cdc25B), calciumcalmodulin-dependent protein kinase II (CaMKII), Wee1, specific phosphorylation status of cyclin-dependent kinase 1 (Cdk1), and cyclin B1 expression levels were analyzed. Our data suggest that the culture of denuded diplotene-arrested oocytes resulted in spontaneous exit from diplotene arrest (EDA) in a time-dependent manner. The supplementation of hCG (2 IU) did not show any additive effect over spontaneous EDA in vitro. The hCG (20 IU) surge induced achievement of meiotic competency as most of the ovulated oocytes showed metaphase-II (M-II) arrest with extrusion of first polar body (PB-I). The spontaneous EDA was associated with a moderate increase of cytosolic free Ca 2+ as well as total ROS levels. Furthermore, iNOS, cAMP, cGMP, Cdc25B, Thr161 phosphorylated Cdk1, as well as cyclin B1 levels were significantly decreased, while CaMKII, Wee1, and Thr14/Tyr15 phosphorylated Cdk1 levels were increased significantly during spontaneous EDA. These changes were reversed in ovulated oocytes that showed M-II arrest. The above results suggest that the oscillation of signal molecules modulates MPF destabilization during the achievement of meiotic competency in rat oocytes cultured in vitro.

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Section: Rosmentioning

confidence: 59%

Spontaneous Exit from Diplotene Arrest Associates with the Increase of Calcium and Reactive Oxygen Species in Rat Oocytes Cultured In Vitro

Tiwari

Chaube

2017

ROS

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“…The MI oocyte can complete the extrusion of its PB after ICSI in human [ 9 ]. A higher proportion of MI oocytes progressed to MII after fertilization in mouse oocytes [ 12 ].…”

Section: Discussionmentioning

confidence: 99%

Compare Two Kinds of Recurrent MI-Arrest Oocytes

Jiang,

Yuan,

Song

et al. 2023

J Obstet Gynecol India

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“…The MI oocytes from patients with a normal number of MII oocytes can fertilization and pregnancy proformed ICSI [16]. A higher proportion of MI oocytes progressed to MII after fertilization in mouse oocytes [17]. The MI oocyte can complete the extrusion of its PB after ICSI in human [16].…”

Section: Discussionmentioning

confidence: 99%

ICSI with All Oocytes Recurrent Metaphase I Characterized by Absence Perivitelline Space

Jiang¹,

Song²,

Yuan³

et al. 2021

OJOG

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Previous cases reported recurrent all oocytes in repeated cycles were arrested at MI failed to mature after culture and ICSI resulted in total nonfertilization. Ionomycin induced first PB extrusion in patients with recurrent maturation-resistant MI oocytes. ICSI combined with assisted oocyte activation (AOA) on MII oocytes matured in vitro resulted in abnormal fertilization with no further embryo cleavage potential. The objective of this work was to research if AOA helpful to fertilization for recurrent maturation-resistant MI oocytes. In this case, patient both cycles retrievaled immature MI oocytes characterized by absence perivitelline space (APVS). In the second cycle, ionomycin was used for AOA after MI oocyte perfomed ICSI, no fertilization again.

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Effects of sperm insemination on the final meiotic maturation of mouse oocytes arrested at metaphase I afterin vitromaturation

Cited by 8 publications

References 26 publications

Spontaneous Exit from Diplotene Arrest Associates with the Increase of Calcium and Reactive Oxygen Species in Rat Oocytes Cultured In Vitro

Spontaneous Exit from Diplotene Arrest Associates with the Increase of Calcium and Reactive Oxygen Species in Rat Oocytes Cultured In Vitro

Compare Two Kinds of Recurrent MI-Arrest Oocytes

ICSI with All Oocytes Recurrent Metaphase I Characterized by Absence Perivitelline Space

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