Effects of sperm pretreatment and embryo activation methods on the development of bovine embryos produced by intracytoplasmic sperm injection

Lee, Jai-Wei; Chang, Hsun-Chung; Wu, Hung‐Yi; Liu, Shyh-Shyan; Wang, Chih-Hua; Chu, Chun-Yen; Shen, Perng-Chih

doi:10.1016/j.repbio.2015.07.001

Cited by 9 publications

(5 citation statements)

References 45 publications

(86 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, the highest rate of embryonic development was observed in this group—a result also reported in a mouse ICSI model using the same sperm pretreatment (Tateno, ). Similar results were recently described using glutathione (Lee et al, ) and lysolecithin (Zambrano et al, ) as sperm pretreatments, confirming the positive effect of pretreatment in promoting DNA decondensation and pronucleus formation. On the other hand, although IBMX increased the sperm membrane fluidity, this treatment showed a similar pronucleus formation rate to the control group, so disrupted membrane integrity alone is not sufficient to improve nuclear condensation following ICSI.…”

Section: Discussionsupporting

confidence: 89%

Sperm capacitation pretreatment positively impacts bovine intracytoplasmic sperm injection

Águila

Zambrano

Arias

et al. 2017

Molecular Reproduction Devel

View full text Add to dashboard Cite

The efficiency of intracytoplasmic sperm injection (ICSI) in bovines is low compared to other species due in part to inadequate egg activation and sperm nucleus decondensation after injection. We hypothesized that this low efficiency is due to the lack of complete sperm capacitation, so we evaluated the effects of isobutylmethylxanthine (IBMX) and methyl-β-cyclodextrin (MβCD) on bovine sperm capacitation and on the preimplantation developmental potential of bovine embryos generated by ICSI. Treatment with IBMX and MβCD decreased sperm viability (between 13-30%); nevertheless, 0.4 mM IBMX and 1 mM MβCD increased (p < 0.05) capacitation metrics-that is, acrosome exocytosis, intracellular calcium level, plasma membrane fluidity, and tyrosine phosphorylation-compared to the control. After ICSI, embryos injected with IBMX- and MβCD-treated sperm showed similar cleavage to the untreated group (range 82-88%). Pronucleus formation rate was higher with MβCD-pretreatment (54%) compared to the control group (25%), and blastocyst rate was significantly improved with MβCD-pretreatment (24%) compared to the IBMX (18%) and control (17%) groups. Importantly, embryo quality-as assessed by the total number of cells, cell allocation, and apoptotic cell index-was not affected by the sperm treatments. In conclusion, MβCD pretreatment of sperm improved the efficiency of blastocyst production in bovine ICSI.

show abstract

Section: Discussionsupporting

confidence: 89%

Sperm capacitation pretreatment positively impacts bovine intracytoplasmic sperm injection

Águila

Zambrano

Arias

et al. 2017

Molecular Reproduction Devel

View full text Add to dashboard Cite

show abstract

“…In addition, DTT predisposes sperm to chromosomal abnormalities [91]. Pre-treatment of sperm with glutathione (GSH) [92], plus oocyte activation by ionomycin in combination with ethanol, improved the efficiency of bovine ICSI. Hence, the detrimental chemical thiol compound pre-treatment (e.g., DTT) can be replaced by glutathione (GSH) [91,92], a major non-protein thiol compound naturally present in mammalian cells.…”

Section: Pre-treatment Of Sperm For Improving the Success Of Bovine Icsimentioning

confidence: 99%

“…Pre-treatment of sperm with glutathione (GSH) [92], plus oocyte activation by ionomycin in combination with ethanol, improved the efficiency of bovine ICSI. Hence, the detrimental chemical thiol compound pre-treatment (e.g., DTT) can be replaced by glutathione (GSH) [91,92], a major non-protein thiol compound naturally present in mammalian cells. GSH is involved in many cellular functions including disulphide bond reduction, protection against oxidative stress, etc.…”

Section: Pre-treatment Of Sperm For Improving the Success Of Bovine Icsimentioning

confidence: 99%

“…In that regard, use of electric stimulation [109], mechanical pre-treatment [80], or chemicals such as ethanol [110], ionomycin, anisomycin (ANY), cycloheximide (CHX), DMAP, dehydroleucodine (DhL) [111] independently or in various combinations, have been reported. Oocyte activation with ethanol alone or in combination with cycloheximide [112,113] or ionomycin [92,114] following ICSI resulted in development of blastocysts. Piezo-driven ICSI more efficient than conventional ICSI [81], and resulted in production of bovine offspring [115,116] when combined with ethanol oocyte activation.…”

Section: Artificial Activation Of Oocyte For Improving the Success Of Bovine Icsimentioning

confidence: 99%

See 1 more Smart Citation

Intracytoplasmic Sperm Injection in Cattle

Unnikrishnan

Kastelic

Thundathil

2021

Genes

View full text Add to dashboard Cite

Intracytoplasmic sperm injection (ICSI) involves the microinjection of sperm into a matured oocyte. Although this reproductive technology is successfully used in humans and many animal species, the efficiency of this procedure is low in the bovine species mainly due to failed oocyte activation following sperm microinjection. This review discusses various reasons for the low efficiency of ICSI in cattle, potential solutions, and future directions for research in this area, emphasizing the contributions of testis-specific isoforms of Na/K-ATPase (ATP1A4) and phospholipase C zeta (PLC ζ). Improving the efficiency of bovine ICSI would benefit the cattle breeding industries by effectively utilizing semen from elite sires at their earliest possible age.

show abstract

“…Another example is glutathione (GSH), an endogenous disulphide bond reducing agent, which plays a fundamental role in the oocyte development from the germinal vesicle stage to metaphase II (Ali, Bilodeau, & Sirard, ; de Matos & Furnus, ). The use of GSH improved the outcome of ICSI in different research studies (Lee et al., ; Sekhavati et al., ).…”

Section: Introductionmentioning

confidence: 99%

Effect of sperm pretreatment with glutathione and membrane destabilizing agents lysolecithin and Triton X‐100, on the efficiency of bovine intracytoplasmic sperm injection

Zambrano

Águila

Arias

et al. 2017

Reprod Domestic Animals

View full text Add to dashboard Cite

Intracytoplasmic sperm injection (ICSI) is an assisted reproduction tool with several applications. Its effectiveness in bovines is lower than that in other species, mainly because of difficulties in the decondensation of the sperm nucleus after injection, and the presence of the acrosome and the plasma membrane which remain intact in this procedure. In this study, we assessed the effect of lysolecithin (LL) and Triton X-100 (TX), in combination with glutathione (GSH) as sperm pretreatments prior to ICSI. The GSH-LL and GSH-TX groups showed 0% of spermatozoa with intact membrane (SYBR 14+/PI), in comparison with the control (63.3%) and GSH (65.7%) groups. The proportions of spermatozoa with damaged acrosome membrane in the GSH-LL, GSH-TX, GSH and control groups were 46%, 35.9%, 10.5% and 7.5%, respectively. Sperm chromatin decondensation analysis showed that the groups incubated for 3 hr with GSH presented greater decondensation (p < .05). Although fertilization was improved in all treatment groups evaluated, no differences were observed in the cleavage rate 72 hr after activation in the GSH (73.7%), GSH-LL (80.2%) and GSH-TX (77.8%) groups compared to the control (66.3%), neither in the blastocyst rate on day 8 (24.0%, 26.2%, 27.1% and 28.4% for the control, GSH, GSH-LL and GSH-TX groups, respectively). No differences were also observed in the total number of cells in all groups. In conclusion, although these sperm treatments promoted nuclear decondensation and induced plasma membrane disruption, these effects were not sufficient to improve bovine embryonic development after ICSI.

show abstract

Effects of sperm pretreatment and embryo activation methods on the development of bovine embryos produced by intracytoplasmic sperm injection

Cited by 9 publications

References 45 publications

Sperm capacitation pretreatment positively impacts bovine intracytoplasmic sperm injection

Sperm capacitation pretreatment positively impacts bovine intracytoplasmic sperm injection

Intracytoplasmic Sperm Injection in Cattle

Effect of sperm pretreatment with glutathione and membrane destabilizing agents lysolecithin and Triton X‐100, on the efficiency of bovine intracytoplasmic sperm injection

Contact Info

Product

Resources

About