Effects of splitting alternative KlCYC1 3′-UTR regions on processing: Metabolic consequences and biotechnological applications

Seoane, Silvia; Guiard, Bernard; Rodríguez-Torres, Ana-María; Freire-Picos, María Ángeles

doi:10.1016/j.jbiotec.2005.03.003

Cited by 8 publications

(8 citation statements)

References 19 publications

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“…It is worth noting that KlCOX5A ( 5B ) showed two transcripts of different size. The meaning of this observation is unknown, but a similar phenomenon was also reported for KlCYC1 [46], [47] and HGT1 [48].…”

Section: Resultssupporting

confidence: 76%

Gene Responses to Oxygen Availability in Kluyveromyces lactis: an Insight on the Evolution of the Oxygen-Responding System in Yeast

et al. 2009

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The whole-genome duplication (WGD) may provide a basis for the emergence of the very characteristic life style of Saccharomyces cerevisiae—its fermentation-oriented physiology and its capacity of growing in anaerobiosis. Indeed, we found an over-representation of oxygen-responding genes in the ohnologs of S. cerevisiae. Many of these duplicated genes are present as aerobic/hypoxic(anaerobic) pairs and form a specialized system responding to changing oxygen availability. HYP2/ANB1 and COX5A/COX5B are such gene pairs, and their unique orthologs in the ‘non-WGD’ Kluyveromyces lactis genome behaved like the aerobic versions of S. cerevisiae. ROX1 encodes a major oxygen-responding regulator in S. cerevisiae. The synteny, structural features and molecular function of putative KlROX1 were shown to be different from that of ROX1. The transition from the K. lactis-type ROX1 to the S. cerevisiae-type ROX1 could link up with the development of anaerobes in the yeast evolution. Bioinformatics and stochastic analyses of the Rox1p-binding site (YYYATTGTTCTC) in the upstream sequences of the S. cerevisiae Rox1p-mediated genes and of the K. lactis orthologs also indicated that K. lactis lacks the specific gene system responding to oxygen limiting environment, which is present in the ‘post-WGD’ genome of S. cerevisiae. These data suggested that the oxygen-responding system was born for the specialized physiology of S. cerevisiae.

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Section: Resultssupporting

confidence: 76%

Gene Responses to Oxygen Availability in Kluyveromyces lactis: an Insight on the Evolution of the Oxygen-Responding System in Yeast

et al. 2009

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“…These isoforms of KlCYC1 mRNA exhibit different fates in response to different physiological conditions, including growth phase, oxygen availability, and the presence or absence of heme (18,41). As cells progress through log phase growth, the S form is degraded, and the L form increases.…”

Section: Discussionmentioning

confidence: 99%

“…These discrete 3Ј-UTR regions were also functional as separate processing units when expressed in S. cerevisiae (18). Indeed, multiple consensus 3Ј-end processing sites are a common feature of S. cerevisiae genes encoding multiple transcripts (45).…”

Section: Discussionmentioning

confidence: 99%

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Promoter-Terminator Gene Loops Affect Alternative 3′-End Processing in Yeast

Lamas-Maceiras

Singh

Hampsey

et al. 2016

Journal of Biological Chemistry

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“…The two KlCYC1 transcripts change their predominance along the growth phase, but not with the carbon source (Freire-Picos et al, 2001). We have shown that when the UTR is split after the first processing point, thereby separating the proximal or distal sequences, they may act as independent processing elements in both S. cerevisiae and K. lactis yeast species (Seoane et al, 2005). The KlCYC1 APA is also conserved when expressed in S. cerevisiae; the separation of the alternative processing positions (395 nucleotides) is a reason to use it as model to study APA in S. cerevisiae.…”

Section: Apa Studies On Klcyc1: Cis Elements and Factors Involvedmentioning

confidence: 99%

Alternative Polyadenylation in Yeast: 3 ́-UTR Elements and Processing Factors Acting at a Distance

Lamas-Maceiras¹,

Seoane²,

Freire-Picos³

2011

RNA Processing

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Effects of splitting alternative KlCYC1 3′-UTR regions on processing: Metabolic consequences and biotechnological applications

Cited by 8 publications

References 19 publications

Gene Responses to Oxygen Availability in Kluyveromyces lactis: an Insight on the Evolution of the Oxygen-Responding System in Yeast

Gene Responses to Oxygen Availability in Kluyveromyces lactis: an Insight on the Evolution of the Oxygen-Responding System in Yeast

Promoter-Terminator Gene Loops Affect Alternative 3′-End Processing in Yeast

Alternative Polyadenylation in Yeast: 3 ́-UTR Elements and Processing Factors Acting at a Distance

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