Effects of spreading areas and aspect ratios of single cells on dedifferentiation of chondrocytes

Cao, Bin; Peng, Rong; Li, Zhenhua; Ding, Jiandong

doi:10.1016/j.biomaterials.2014.04.107

Cited by 70 publications

(70 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…White arrows indicate the deposited CSPG. (B) F CSPG of P3 chondrocytes on different substrates during the course imaging, this allowed relative quantitation of flu-494 orescence intensity per pattern and thereby objective comparison of 495 the total production of chondrogentic matrix by individual cells.496 This approach revealed that a spherical cell shape is beneficial for 497 the production of CSPG and collagen II, and importantly, this influ-498 ence could occur on a 2D substrate, at the very early stages term culture, as reported by recent work where single chon-503 drocytes were cultured on round RGD patterns (from 10 lm to 504 30 lm diameter) for up to 10 days[30]. Dedifferentiation occurred505 for cells on all patterns, and the probability of dedifferentiation506 …”

mentioning

confidence: 76%

“…In combination increased for larger patterns (e.g. $65% on 30 lm dots) [30]. 507 The present work, as discussed below, illustrates that this trend 508 is different in 3D settings.…”

Section: Introductionmentioning

confidence: 84%

“…Using single cell patterning, various 83 studies show that cell shape and area both control cell growth, 84 death [28,31,33], and regulation of stem cell differentiation 85 [27,29]. Surprisingly, studies concerning single chondrocytes are 86 limited [30]. However, how cell geometry in 3D affects cell function 87 remains illusive.…”

Section: Introductionmentioning

confidence: 99%

“…Methods that can precisely control the 77 microenvironments of chondrocytes are needed for in-depth 78 understanding of the effects of cell geometry on the chondrocyte 79 phenotype. 80 In the last decade, micropatterning has become a versatile tool 81 for the study of geometrical influences on cell function at the single 82 cell level in 2D culture [27][28][29][30][31][32]. Using single cell patterning, various 83 studies show that cell shape and area both control cell growth, 84 death [28,31,33], and regulation of stem cell differentiation 85 [27,29].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

A novel culture system for modulating single cell geometry in 3D

et al. 2015

View full text Add to dashboard Cite

Dedifferentiation of chondrocytes during in vitro expansion remains an unsolved challenge for repairing serious articular cartilage defects. In this study, a novel culture system was developed to modulate single cell geometry in 3D and investigate its effects on the chondrocyte phenotype. The approach uses 2D micropatterns followed by in situ hydrogel formation to constrain single cell shape and spreading. This enables independent control of cell geometry and extracellular matrix. Using collagen I matrix, we demonstrated the formation of a biomimetic collagenous "basket" enveloping individual chondrocytes cells. By quantitatively monitoring the production by single cells of chondrogenic matrix (e.g. collagen II and aggrecan) during 21-day cultures, we found that if the cell's volume decreases, then so does its cell resistance to dedifferentiation (even if the cells remain spherical). Conversely, if the volume of spherical cells remains constant (after an initial decrease), then not only do the cells retain their differentiated status, but previously de-differentiated redifferentiate and regain a chondrocyte phenotype. The approach described here can be readily applied to pluripotent cells, offering a versatile platform in the search for niches toward either self-renewal or targeted differentiation.

show abstract

mentioning

confidence: 76%

“…In combination increased for larger patterns (e.g. $65% on 30 lm dots) [30]. 507 The present work, as discussed below, illustrates that this trend 508 is different in 3D settings.…”

Section: Introductionmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A novel culture system for modulating single cell geometry in 3D

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Apart from gold, glass, and silicon surface, soft surfaces such as hydrogels have been utilized to pattern cells. Cao et al [13] generated a series of microarrays of cell-adhesive RGD on a persistent non-fouling PEG hydrogel and researched the effects of spreading areas and aspect ratios of single cells on dedifferentiation of chondrocytes.…”

Section: Patterning Cells With Inert and Adsorptive Surfacesmentioning

confidence: 99%

Precise manipulation of cell behaviors on surfaces for construction of tissue/organs

Zheng

Jiang

2014

Colloids and Surfaces B: Biointerfaces

View full text Add to dashboard Cite

Semi‐Egg‐Like Heterogeneous Compartmentalization of Cells Controlled by Contact Angle Hysteresis

Sun

Liu

et al. 2015

Adv Funct Materials

View full text Add to dashboard Cite

Precise control of liquid inside compartments is critically important in bioreactors, combinatorial analysis, and tissue engineering. A contact angle hysteresis (CAH)-based strategy is demonstrated to construct a semi-egg-like hydrogel architecture, leading to spatial heterogeneous compartmentalization of cells. The semi-egg-like architecture is fabricated by successively capturing and gelling prehydrogel liquids using a substrate with controlled-CAH pattern and ultralow-CAH background. The controlled-CAH pattern could capture liquid with tunable size, while ultralow-CAH background prevents liquid sticking. It is envisioned that this CAH-based strategy would be promising in designing functional surface for engineering complicated architectures of either biomedical or nonbiomedical systems.

show abstract

Effects of spreading areas and aspect ratios of single cells on dedifferentiation of chondrocytes

Cited by 70 publications

References 69 publications

A novel culture system for modulating single cell geometry in 3D

A novel culture system for modulating single cell geometry in 3D

Precise manipulation of cell behaviors on surfaces for construction of tissue/organs

Semi‐Egg‐Like Heterogeneous Compartmentalization of Cells Controlled by Contact Angle Hysteresis

Contact Info

Product

Resources

About