Effects of structure on the performance of latex nanoparticles as a pseudostationary phase in electrokinetic chromatography

Hyslop, Jesse S.; McGettrick, Julie R.; Hall, Leah M. G.; Chuk, Hungngai; Palmer, Christopher P.

doi:10.1016/j.aca.2017.11.020

Cited by 2 publications

(1 citation statement)

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“…A typical function of AMPS is to accelerate EOF in capillary electrochromatography either in the form of an additive to the monolith composition [19][20][21] or as a grafted polymer to the monolith surface [22,23]. In electrokinetic chromatography, AMPS was used as an additive or a comonomer in novel pseudo-stationary phases to alter their polarity and EOF [24][25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Covalent anionic copolymer coatings with tunable electroosmotic flow for optimization of capillary electrophoretic separations

et al. 2022

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We present a method for finely adjustable electroosmotic flow (EOF) velocity in cathodic direction for the optimization of separations in capillary electrophoresis. To this end, we use surface modification of the separation fused silica capillary by the covalently attached copolymer of acrylamide (AM) and 2‐acrylamido‐2‐methyl‐1‐propanesulfonate (AMPS), that is, poly(AM‐co‐AMPS) or PAMAMPS. Coatings were formed by the in‐capillary polymerization of a mixture of the neutral AM and anionic AMPS monomers premixed in various ratios in order to control the charge density of the copolymer. EOF mobility varies in the 0 to ∼40 × 10−9 m2 V−1 s−1 interval for PAMAMPS coatings ranging from 0 to 60 mol.% of charged AMPS monomer. For EOF in PAMAMPS‐treated capillaries, we observed (i) a negligible dependence on pH in the 2–10 interval, (ii) a minor variance among background electrolytes (BGEs) in function of their components and (iii) its standard decrease with increasing ionic strength of the BGE. Interest in variable cathodic EOF was demonstrated by the amelioration of separation of two kinds of isomeric anionic analytes, that is, monosaccharides phosphates and helquat enantiomers, in counter‐EOF mode.

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