Effects of subacute treatment of ethylene glycol on serum marker enzymes and erythrocyte and tissue antioxidant defense systems and lipid peroxidation in rats

Çelik, İsmail; Suzek, Huseyin

doi:10.1016/j.cbi.2007.02.007

Cited by 20 publications

(15 citation statements)

References 16 publications

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“…It has already been proven that EG-induced hyperoxaluria caused LPO with a concomitant decrease in activities of antioxidant enzymes, namely SOD and CAT (Celik & Suzek, 2007). It was reported that hyperoxaluria enhances the production of free radicals with subsequent LPO in the kidneys, leading to renal tubular epithelial cell injury and calcium oxalate stone formation (Moriyama et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Antioxidative mechanism involved in the preventive efficacy ofBergenia ciliatarhizomes against experimental nephrolithiasis in rats

Saha¹,

Shrivastav

Verma³

2014

Pharmaceutical Biology

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Context: Bergenia ciliata Haw. (Saxifragaceae) is widely used in traditional medicines for renal disorders including kidney stones, inflammation and also well known for its antioxidant activity. Use of traditional herbs proved to be an important strategy for the management of kidney stones by modulating the oxidative stress imposed by calcium oxalate nephrolithiasis. Objectives: To evaluate the antinephrolithiatic and antioxidative activity of B. ciliata rhizomes as a preventive agent on ethylene glycol (EG)-induced nephrolithiasis. Materials and methods: The hydro-methanol extract (30:70, v/v) of B. ciliata rhizomes was orally administrated simultaneously at a dose of 150 and 300 mg/kg body weight/day, to adult female Wistar rats for 28 d along with EG (0.75%, v/v) in drinking water. The results were compared to a parallel study conducted with marketed polyherbal drug cystone under identical dosage conditions. The biochemical parameters were measured in urine, serum and kidney followed by histochemistry. A validated HPLC method was used for standardization using gallic acid as a marker.Results: EG caused a significant increase in calcium, oxalate and phosphate levels in urine and kidney and concurrent decrease in calcium, sodium and magnesium in serum (p50.001). EG also caused an increase in lipid peroxidation and a decrease in activities of antioxidative enzymes in kidney. Co-treatment with B. ciliata rhizomes extract caused restoration of all these parameters (p50.001). Histochemical studies showed reduced calcifications with extract treatment. Conclusion: B. ciliata has a significant prophylactic effect in preventing the nephrolithiasis, which might be mediated through antioxidant activity of these active compounds.

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Section: Discussionmentioning

confidence: 99%

Antioxidative mechanism involved in the preventive efficacy ofBergenia ciliatarhizomes against experimental nephrolithiasis in rats

Saha¹,

Shrivastav

Verma³

2014

Pharmaceutical Biology

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“…Excessive ROS production that overcomes the mopping of these ROS by endogenous antioxidants is linked to male infertility [ 41 ]. It has been reported that the administration of EGEs leads to a steady production of ROS [ 42 ] and alterations in the anti-oxidative systems by either raising or lowering the levels of antioxidant defense systems through ROS production [ 43 ]. In this study, the significant increase in testis MDA concentration after 21 days of EGME administrations ( Fig.…”

Section: Discussionmentioning

confidence: 99%

“…3 C) activity following EGME administrations, can be attributed to testicular response to EGME-induced free radical generation and oxidative stress. It has been reported that an increase in the activity of GST is known to serve as protective responses to eliminate xenobiotics [ 43 , 57 ]. Also reported was that exposure to ethylene glycol monoethyl ether (EGEE), a member of the ethylene glycol ether family, resulted into a decrease in the testicular level of GSH [ 19 , 56 , 58 ], activities of GPx [ 58 ], and SOD [ 19 , 58 ], as well as a significant increase in testicular GST [ 19 , 58 ] activity in rats.…”

Section: Discussionmentioning

confidence: 99%

Ethylene glycol monomethyl ether-induced testicular oxidative stress and time-dependent up-regulation of apoptotic, pro-inflammatory, and oncogenic markers in rats

et al. 2020

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Ethylene glycol monomethyl ether (EGME) is a major component of paints, lacquers, inks, and automobile brake fluids. As a result, exposures to humans are inevitable. We therefore, investigated in this study, its effect on testicular cells in a time-course manner in male Wistar rats. Animals were orally administered 50 mg/kg body weight of EGME for duration of 7, 14, and 21 days. Following 7 days of the administration, levels of NO and GSH were significantly reduced, while levels of c-Myc, K-Ras, caspase-3, IL-6, TNF-α, and IL-1β were significantly increased compared with control. At the end of 14 days exposure, GPx, and SOD activities, as well as IL-10 level were significantly decreased, while levels of c-Myc, K-Ras, p53, Bax, caspase-3, IL-6, TNF-α, IL-1β, and GST activity were significantly elevated compared with control. After 21 days of EGME administration, Bcl-2, IL-10, and NO levels were significantly decreased, while levels of c-Myc, K-Ras, p53, Bax, caspase-3, IL-6, TNF-α, IL-1β, MDA and GST activity were significantly increased compared with control. After 7, 14, and 21 days of EGME administrations, testis histopathology showed severe loss of seminiferous tubules, the seminiferous epithelium revealed very few spermatocytes, spermatids, spermatogonia, spermatozoa, and Sertoli cells, while the interstitial tissue is eroded, with scanty abnormal Leydig cells, compared with the control that appeared normal. We therefore, concluded that EGME-induced testicular toxicity as a result of EGME administration could be via the disorganization of the endogenous antioxidant systems as well as up-regulation of pro-inflammatory, apoptotic and oncogenic mediators in rats.

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“…These findings indicate that rats supplemented with spirulina for the last three weeks were mostly recovered from nephrolithiasis and completely from hepatotoxicity induced by ethylene glycol at the first three weeks. Nephrolithiasis and hepatotoxicity induction by ethylene glycol was established in many researches (Christina et al, 2002;Atmani et al, 2003;Huang et al, 2006;Karadi et al, 2006;Celik and Suzek, 2007;Hadjzadeh et al, 2007;Hadjzadeh et al, 2008;Verma et al, 2009;Divaka et al, 2010). The mechanism underlying the effect of spirulina on nephrolithiasis induced by ethylene glycol is still unknown, but is apparently related to increased diuresis and lowering of urinary concentrations of stone forming constituents.…”

Section: Discussionmentioning

confidence: 99%

Antilithiatic Influence of Spirulina on Ethylene Glycol-Induced Nephrolithiasis in Male Rats

Al-Attar¹

2010

American Journal of Biochemistry and Biotechnology

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Problem statement: Nephrolithiasis or renal stone disease remains a significant health problem in the adult population. Nephrolithiasis is a recurrent disorder prominent in males. It is significant medical and surgical problem because of incidence, recurrence and severe consequences. The present day medical management of nephrolithiasis is either costly or not without side effects. Invasive procedures for the treatment of nephrolithiasis may cause serious complications and they also impose a great load of costs to the healthcare system. Hence the search for antilithiatic drugs from natural sources has assumed greater importance. Approach: The aim of the present study was to evaluate the antilithiatic activity of spirulina supplementation on ethylene glycol induced nephrolithiasis in male rats. Healthy male Wistar rats were used in the present study and were divided randomly into 4 groups. Rats of 1st group were served as normal control. Rats of 2nd group were received 0.75% ethylene glycol in drinking water for three weeks and drinking tap water for the next three weeks. Rats of 3rd group were received 0.75% ethylene glycol in drinking water for three weeks and fed with spirulina solution (20 mg kg −1 body weight) for the next three weeks. Animals of 4th group were treated as 1st group for the first three weeks and fed with spirulina solution ate the same dose given to 3rd group for the last three weeks. After six weeks, serum levels of sodium, chloride, potassium, calcium, phosphorus, Blood Urea Nitrogen (BUN), uric acid, creatinine, Alanine aminotransferase (ALT), Aspartate aminotransferase (AST) were measured. Results: Statistically increases in the levels of sodium, chloride, BUN and ALT and a decrease in the level of calcium were noted in rats treated with ethylene glycol. Supplementation of spirulina for the last three weeks mostly recovered the rats from nephrolithiasis and completely from hepatotoxicity induced by ethylene glycol. Conclusion:This study suggested that spirulina is a safety and promising agent as a functional food for the management of nephrolithiasis induced by ethylene glycol and may be also by other chemical factors.

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Effects of subacute treatment of ethylene glycol on serum marker enzymes and erythrocyte and tissue antioxidant defense systems and lipid peroxidation in rats

Cited by 20 publications

References 16 publications

Antioxidative mechanism involved in the preventive efficacy ofBergenia ciliatarhizomes against experimental nephrolithiasis in rats

Antioxidative mechanism involved in the preventive efficacy ofBergenia ciliatarhizomes against experimental nephrolithiasis in rats

Ethylene glycol monomethyl ether-induced testicular oxidative stress and time-dependent up-regulation of apoptotic, pro-inflammatory, and oncogenic markers in rats

Antilithiatic Influence of Spirulina on Ethylene Glycol-Induced Nephrolithiasis in Male Rats

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