Effects of subinhibitory concentrations of nitroxoline on the surface properties ofEscherichia coli

Latrache, Hassan; Bourlioux, P.; Karroua, M.; Zahir, Hafida; Hakkou, Abdelkader

doi:10.1007/bf02818714

Cited by 19 publications

(15 citation statements)

References 27 publications

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“…The values we obtained for C-(O, N)/C (0.337 and 0.314 for free and bound EPS respectively, Table 3) are higher than those reported for proteins (0.26-0.293) but lower than those reported for polysaccharides (0.833). [47][48][49][50] Similarly, (CdO)/C + (O-C-O)/C values for free and bound EPS (0.144 and 0.141, respectively) are close to those reported for polysaccharides (0.167) and lower than those for proteins (0.225-0.279). Atomic ratios N/C, O/C, and P/C are higher for bound EPS, whereas N/P is higher in free EPS (Table 3).…”

Section: Resultssupporting

confidence: 80%

Spectroscopic Study of Extracellular Polymeric Substances from Bacillus subtilis: Aqueous Chemistry and Adsorption Effects

2004

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Reactions at ionizable functional groups in extracellular polymeric substances (EPS) from Bacillus subtilis are found to affect aqueous phase conformation and adsorption to mineral surfaces. Characterization by HPSEC, XPS, and FTIR indicates a wide range in apparent molecular mass (0.57-128 kDa), with functional group composition depending on cell growth phase (exponential vs stationary) and location in suspension (free vs cell-bound). ATR-FTIR spectroscopy shows complexation and dissociation of protons on acidic functional groups that result in alpha-helical protein conformation at pH < 2.6 and random coil (unordered) conformation at higher pH (>6). EPS exhibit higher affinity for adsorption to alpha-FeOOH than amorphous SiO(2) because of surface charge effects. Increased amide II band intensity and an amide I band shift to higher frequency indicate changes in protein structure upon adsorption. Goethite-EPS spectra show emergent vibrations consistent with P-O-Fe bonding, which suggests a role of phosphodiester groups in the adsorption reaction.

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Section: Resultssupporting

confidence: 80%

Spectroscopic Study of Extracellular Polymeric Substances from Bacillus subtilis: Aqueous Chemistry and Adsorption Effects

2004

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“…These effects are not limited to E. coli strains, but also occur with many different bacteria [1][2][3][4][5][6]. Several researchers have reported that sub-MICs of some antibiotics increase the surface hydrophobicity and the ability of bacteria to adhere to epithelial cells [13,14].…”

Section: Discussionmentioning

confidence: 99%

Effects of subinhibitory concentrations of amikacin and ciprofloxacin on the hydrophobicity and adherence to epithelial cells of uropathogenic Escherichia coli strains

Wojnicz

Jankowski

2007

International Journal of Antimicrobial Agents

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“…Many authors have described the effects of these environmental parameters on hydrophobicity and charge (Beck et al 1988;Herben et al 1990;Van Der Mei et al 1993;Latrache et al 1994;Briandet et al 1999;Latrache et al 2000). Literature data (Rouxhet and Mozes 1990) reported that the hydrophobicity and charge were insufficient to explain the adhesion phenomenon.…”

Section: Introductionmentioning

confidence: 94%

Atomic force microscopy and hydrodynamic characterization of the adhesion of staphylococcus aureus to hydrophilic and hydrophobic substrata at different pH values

Zmantar

Bettaieb

Chaieb

et al. 2010

World J Microbiol Biotechnol

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Understanding the mechanism of the bacterial cell adhesion to solid surfaces is of great medical and industrial importance. Bacterial adhesion to inert surfaces, such as a catheter, and other indwelling devices can form biofilm, consequently cause severe morbidity and often fatal infections. Initial bacterial adhesion to the material surfaces is a complicated process that is affected by various physicochemical properties of both bacterial cells and substratum surfaces. The surface properties of the cells were characterized by the sessile drop technique. Moreover, the interfacial free energy of Staphylococcus aureus adhesion to the supporting materials was determined. The results showed that S. aureus examined at different pH levels could be considered hydrophilic. We noted hat the electron-donor character of S. aureus was important at intermediate pH (pH 5, pH 7, and pH 9) and it decreased at both limits acidic and basic conditions. In addition, the adhesion of Staphylococcus aureus ATCC 25923 to the hydrophilic glass and hydrophobic indium tin oxide (ITO)-coated glass surfaces at different pH values (2, 3, 5, 7, 9 and 11) was investigated using atomic force microscopy (AFM) and image analysis was assessed with the Mathlab Ò program. The data analysis showed that cells (number of adhering cells to glass and ITO-coated glass surface) adhered strongly at acidic pH and weakly at alkaline pH. Also, S. aureus has the ability to attach to both hydrophobic and hydrophilic surfaces, but the adhesion was higher on hydrophobic surface.

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Effects of subinhibitory concentrations of nitroxoline on the surface properties ofEscherichia coli

Cited by 19 publications

References 27 publications

Spectroscopic Study of Extracellular Polymeric Substances from Bacillus subtilis: Aqueous Chemistry and Adsorption Effects

Spectroscopic Study of Extracellular Polymeric Substances from Bacillus subtilis: Aqueous Chemistry and Adsorption Effects

Effects of subinhibitory concentrations of amikacin and ciprofloxacin on the hydrophobicity and adherence to epithelial cells of uropathogenic Escherichia coli strains

Atomic force microscopy and hydrodynamic characterization of the adhesion of staphylococcus aureus to hydrophilic and hydrophobic substrata at different pH values

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