Effects of Sucralfate, Cimetidine and Rabeprazole on Mucosal Hydroxyproline Content in Healing of Ethanol–hcl‐induced Gastric Lesions

Abstract

1. No general consensus has been reached on the treatment of acute gastric lesions. The aims of the present study were to clarify the effects of sucralfate, cimetidine and rabeprazole monotherapies and combination therapies on acute gastric lesions from the viewpoint of connective tissue regeneration. 2. Gastric lesions were experimentally created by the oral administration of 50% ethanol-0.15 mol/L HCl to rats. After 30 min, the anti-ulcer agents sucralfate (100 mg/kg), cimetidine (20 mg/kg) and rabeprazole (2 mg/kg) were administered separately or in combination and the stomach was excised at different times to measure the level of hydroxyproline in the gastric mucosa and determine lesion index. Immunostaining against prolylhydroxylase was performed on some specimens. 3. In the control group, lesion index decreased linearly from 30 min after ethanol-HCl administration and the level of mucosal hydroxyproline peaked between 2 and 4 h later. Although sucralfate significantly promoted lesion healing, it had no effect on mucosal hydroxyproline level. Cimetidine suppressed increases in mucosal hydroxyproline and prolonged lesion healing, but these findings were reversed by combining cimetidine and sucralfate. Rabeprazole had no significant effect on lesion healing, but promoted lesion healing in combination with sucralfate. Immunohistochemical analysis showed that prolylhydroxylase was expressed in spindle cells that lined the glandular cells in a boundary area between normal and injured tissues. 4. Under conditions in which the effects of intragastric pH are minimal, sucralfate is superior to antisecretory agents in promoting the healing of acute gastric lesions.

Methylene Blue Protects against Acidified Sodium Taurocholate-Induced Gastric Mucosal Damage

“…This increase in gastric hydroxyproline was reduced but not normalized by MethyB. Other researchers found increased hydroxyproline in gastric mucosa 2 and 4 h after intragastric administration of 50% ethanol-0.15 M HCl to rats compared with the untreated gastric mucosa [57]. We also showed that Na + -taurocholate resulted in extensive decrease in PAS + surface epithelial cells and gastric glands, indicating depletion of gastric mucus.…”

Gastroprotective effect of Cissus sicyoides (Vitaceae): Involvement of microcirculation, endogenous sulfhydryls and nitric oxide

“…When we compared to the control value, Cc significantly inhibited ulcerative lesions in all doses, but the best result was obtained after pretreatment with Cc using the absolute ethanol model (Table 1). EtOH-induced ulcers are not inhibited by antisecretory agents such as cimetidine, but are inhibited by agents that exhibit a gastroprotective action with an anti-oxidative cytoprotection (Arisawa et al, 2006). Therefore, our results suggest that Cc has an antiulcerogenic effect related to cytoprotection.…”

The Influence of Melanin on the Gastric Mucosa and Hypothalamicpituitary-Adrenocortical Axis Under Acute Stress Conditions

“…[4] показали важливість оксипроліну як одного з основних маркерів ульцерогенезу за умов водно-іммобілізаційного стресу. Тоді як інші автори [18] відмічають необхідність колагену у клітинній диференціації та швидкості гоєння виразкових уражень при етаноліндукованому ульцерогенезі. Отже, меланін може виступати важливим елементом захисту структурної цілісності СОШ за умов дії стресу різного генезу.…”