Effects of sugar cane extract on steroidogenesis in testicular interstitial cells of male Japanese quail (Coturnix japonica)

Abstract

Sugar cane extract (SCE) is the end product of glucose, fructose, and sucrose elimination in molasses. SCE has various biological effects, such as anti-inflammation and antioxidation, and it is commonly found in animal feed. The present research is aimed at investigating the reproductive endocrine influence of SCE in male Japanese quails (Coturnix japonica) by feeding SCE containing food. In addition, in vitro Leydig cell culture was conducted to clarify the mechanism of SCE's influence. Our results showed that SCE feed extended the latency to the first neck grab, decreased male quail testis and epididymis weights, cloaca gland size, and reduced serum testosterone concentrations. Steroidogenic enzymes 3βHSD, 17βHSD, P450c17, and P450scc gene expression in the testis were decreased in the SCE groups.Western blot analysis showed decreased 3βHSD in the testis after feeding SCE.Isolated testicular interstitial cells cultured with SCE and ovine-LH suppressed testosterone secretion and 3βHSD gene expression. In conclusion, SCE as a feed additive has an impact on the sexual behavior and reproductive function of male Japanese quail, with the suppression of steroidogenesis in the Leydig cell. Our results may provide beneficial information to the livestock management and the poultry industry.

From feed to fork: immunity, performance and quality of products from farm animals fed sugarcane products

“…was carried out on male quail after supplementing their feed with an extract derived from sugarcane molasses, where the effects of reproductive endocrine activity was evaluated. The study reported a decrease in serum testosterone, and testicular and epidydimal weight, an inhibition of hydroxysteroid dehydrogenase and suppressed sexual behaviour, suggesting the ability of sugarcane molasses supplementation on impeding steroidogenesis (Pu et al, 2022). The authors concluded that this was useful in the poultry industry in managing livestock, as decreased testosterone leads to efficient feed to growth conversion, as well as contributing to improved quality of meat (Rikimaru et al, 2009).…”

“…Regarding animal performance, a study by Pu et al (2022). was carried out on male quail after supplementing their feed with an extract derived from sugarcane molasses, where the effects of reproductive endocrine activity was evaluated.…”