Effects of sulpiride on mRNA levels of steroid 5α‐reductase isozymes in prostate of adult rats

Sánchez, Pablo Tercedor; Torres, Juan Antonio Vera; Vílchez, Pablo; Moral, Raimundo García del; Ortega, Esperanza

doi:10.1002/iub.13

Cited by 5 publications

(2 citation statements)

References 46 publications

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“…Our group previously found sulpiride‐induced hyperprolactinemia to be associated with increased mRNA levels of both 5α‐R1 and 5α‐R2 in the prostate of adult rats , in line with findings of higher 5α‐R activity in the rat prostate after administration of ovine PRL . Our PRL results are consistent with reports of prostate hyperplasia in transgenic mice that overexpress the PRL gene , and of a smaller prostate in PRL‐null mice than in wild‐type counterparts .…”

Section: Discussionsupporting

confidence: 91%

Expression of steroid 5α‐reductase isozymes in prostate of adult rats after environmental stress

et al. 2012

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The elevated incidence of prostate cancer and benign prostatic hypertrophy is a cause of increasing public health concern in the Western world. The normal and pathological growth of the prostate are both dependent on stimulation by dihydrotestosterone, which is synthesized from circulating testosterone by two 5α‐reductase (5α‐R) isozymes, 5α‐reductase type 1 (5α‐R1) and 5α‐reductase type 2 (5α‐R2). Both isozymes have been implicated in prostate disease. We used quantitative RT‐PCR and immunohistochemistry, respectively, to quantify mRNA and protein levels of 5α‐R isozymes in the ventral prostate of adult rats under environmental stress conditions analogous to those found in some common workplace situations, i.e. artificial light, excessive heat, and the sensation of immobility in a small space. Transcription and expression levels of both 5α‐R isozymes were significantly higher in environmentally stressed rats than in unstressed rats. Increased 5α‐R isozyme levels may play a role in the development or maintenance of prostate disease. Further research is warranted to explore these effects of environmental stress on human health and their implications for environmental and occupational health policies.

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Section: Discussionsupporting

confidence: 91%

Expression of steroid 5α‐reductase isozymes in prostate of adult rats after environmental stress

et al. 2012

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“…Experimental groups. Rats were randomly divided into three groups (n=12) according to body weight following acclimatization, treated daily with sulpiride (40 and 120 mg/kg, intragastrically) or vehicle (0.5% CMC-Na) for 4 weeks and weighed once per week as previously described (42). The 40 and 120 mg/kg dosages represent the therapeutic low and high dose used in human treatment (43,44).…”

Section: Animals and Housingmentioning

confidence: 99%

Oral exposure of sulpiride promotes the proliferation of Brown‑Norway rat prostates

et al. 2020

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The aim of the present study was to establish an animal model of prostatic hyperplasia to explore the mechanisms of this disease. Sulpiride, a specific type 2 dopamine receptor antagonist, causes prostate toxicity by stimulating prolactin (PRL) production. Male Brown-Norway (BN) rats were treated intragastrically (i.g.) with sulpiride (40 and 120 mg/kg daily) and vehicle (i.g., daily) for 4 weeks. The results demonstrated that sulpiride-treatment resulted in increased prostate size, prostate lobe weight, epithelial height and acinar luminal area. Furthermore, prostate lobe weight, epithelial height and acinar luminal area of lateral lobes (LP) significantly increased. These effects were dose dependent.

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Effects of metoclopramide on mRNA levels of steroid 5α-reductase isozymes in prostate of adult rats

et al. 2012

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The rising incidence of prostate cancer and benign prostatic hypertrophy in the Western world is a cause of increasing public health concern. The most active androgen in the prostate is 5α-dihydrotestosterone obtained from testosterone (T) by the enzyme 5α-reductase (5α-R), expressed in the prostate as two isozymes, 5α-R1 and 5α-R2. These isozymes are involved in the growth and development of normal prostate and in the onset and progression of prostate disease. Besides androgens, prolactin (PRL) may also play a role, although it is not clear whether its effects on the prostate are in synergism with or independent of those of androgens. We previously demonstrated that sulpiride, an inductor of hyperprolactinemia, increased mRNA levels of 5α-R isozymes in prostate of adult rat. We hypothesized a possible interrelationship between PRL levels and 5α-R, although the effects of sulpiride per se cannot be ruled out. In the present study, one-step quantitative reverse transcription polymerase chain reaction coupled with laser-induced fluorescence capillary electrophoresis was used to quantify mRNA levels of both 5α-R isozymes in prostate of adult rat after administration of metoclopramide (MTC), another inductor of PRL secretion. With the administration regimens studied, MTC produced an increase in prostate weight and mRNA levels of 5α-R1 and 5α-R2 in adult rats. Given our finding that MTC per se or MTC-induced hyperprolactinemia modifies prostate disease-related parameters in animals with reduced plasma T levels, further investigation is warranted into the possibility that MTC use by aging males may increase their risk of prostate disease.

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Effects of sulpiride on mRNA levels of steroid 5α‐reductase isozymes in prostate of adult rats

Cited by 5 publications

References 46 publications

Expression of steroid 5α‐reductase isozymes in prostate of adult rats after environmental stress

Expression of steroid 5α‐reductase isozymes in prostate of adult rats after environmental stress

Oral exposure of sulpiride promotes the proliferation of Brown‑Norway rat prostates

Effects of metoclopramide on mRNA levels of steroid 5α-reductase isozymes in prostate of adult rats

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