Tomato plants in greenhouse production are often confronted with light insufficiency in the lower canopy, especially in the winter low irradiation season. In this study, periodic alteration of plant density (PD) was proposed to improve the light environment of plants' lower canopies, leaf photosynthesis, plant growth, and fruit development. These were investigated with winter cultivated tomatoes in a single-truss production system. Seedlings were transplanted to either movable or fixed cultivation benches for treatments with fixed PDs of 14.3 plants·m at mature green to red-ripe). The leaf photosynthesis rates in MB and F10 were generally significantly higher than in F14.3. F14.3 had the tallest stems, but the lowest leaf area and shoot dry weight at end of experiment, while MB and F10 were not significantly different. MB had significantly the highest total yield, while the lowest data were observed in F10. The soluble solids content was increased in MB and F10 compared with F14.3, but no significant differences in ascorbic acid content or fruit hardness were observed among treatments. Therefore, a high PD in the vegetative stage, but a relatively lower PD in the fruit development stage, was highly efficient in intensive tomato cultivation.