Effects of supplemental rumen-protected conjugated linoleic acid or corn oil on fatty acid composition of adipose tissues in beef cattle1

Gillis, M. H.; Duckett, S. K.; Sackmann, J. R.

doi:10.2527/2004.8251419x

Cited by 75 publications

(66 citation statements)

References 33 publications

(46 reference statements)

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“…Gillis et al (2004) reported lower concentration of monounsaturated fatty acids in the meat from calves fed lipid-rich diets. There Regarding the sensorial analysis (Table 5), it is verified that the cottonseed addition over 34.09 kg for every 100 kg of the diet (P<0.05) resulted in slightly more intense strange flavor.…”

Section: Resultsmentioning

confidence: 99%

Meat characteristics of Nellore steers fed whole cottonseed

et al. 2013

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-The objective of this study was to evaluate the effect of addition of different levels of cottonseed (0, 14.35, 27.51 and 34.09 kg/100 kg) in the diet of Nellore steers on the meat characteristics. Thirty-six steers (average initial body weight of 333.50 and average age of 20 months) were kept in feedlot stalls for 94 days, each stall with three animals. The experiment was a completely randomized design with four treatments and nine replications. The values of protein, fixed mineral residue, shear force, lipid oxidation, meat color and fat color were similar among the treatments. The increase of the cottonseed levels in the diet reduced the amount of intramuscular fat and increased the moisture content in the meat linearly. Meat aroma and flavor were negatively influenced when cottonseed addition was greater than 27.51 and 34.09 kg/100 kg of the diet, respectively. Cottonseed diet did not modify the concentration of conjugated linoleic acid, saturated fatty acids or total unsaturated fatty acids, but linearly reduced the concentration of monounsaturated fatty acids in the meat.

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Section: Resultsmentioning

confidence: 99%

Meat characteristics of Nellore steers fed whole cottonseed

et al. 2013

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“…However, there are also experiments that report a negative effect of CLA mixtures on adipogenesis, as measured by the expression levels of PPARγ and C/EBPα and the activity of glycerol-3-phosphate dehydrogenase [Griswold et al, 2003]. It is noteworthy that the isomer composition of the CLA added in these and other experiments [Evans et al, 2000;Gillis et al, 2004] was not identified, which could account for the conflicting results.…”

Section: Discussionmentioning

confidence: 50%

PPAR Agonists Promote the Differentiation of Porcine Bone Marrow Mesenchymal Stem Cells into the Adipogenic and Myogenic Lineages

Pérez-Serrano

González-Dávalos²,

Lozano-Flores³

et al. 2016

Cells Tissues Organs

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Purpose: The aim of this work was to evaluate the effect of PPAR agonists on the differentiation and metabolic features of porcine mesenchymal stem cells induced to the adipogenic or myogenic lineages. Methods: Bone marrow MSCs from neonate pigs were isolated and identified by cell proliferation, cell surface markers or the gene expression of stem cells (CD44, CD90, CD105 or Oct4 and Nanog, respectively). Cells were differentiated into adipose or muscle cells and treated with the PPAR agonists; adipogenic and myogenic differentiation was promoted by adding these compounds. The expression of PPARγ (an adipose marker) and MyoD1 and MyHC (muscle markers), metabolic changes and expression levels of metabolic enzymes involved in glycolysis, lipogenesis, lipolysis and the pentose phosphate pathway were tested by qPCR. Results: MSCs from neonate pigs exhibited high proliferation and were positive for CD44, CD90 and CD105 markers and Oct4 and Nanog expression. The treatment that promoted the highest expression of PPARγ was 50 µM of conjugated linoleic acid (CLA) c9 t11 (6.44 ± 0.69-fold, p ≤ 0.0001) in the adipose differentiation, and upregulation of HX2, ACCAα, ATGL, LPL and G6DP (p ≤ 0.0001) and downregulation of PFK and ACCAβ (p ≤ 0.0001) were found. For muscle differentiation, the best treatment was 50 µM of CLA c10 t12 (59.72 ± 4.72-fold, p ≤ 0.0001), and metabolic changes were upregulation of PFK, ACCAβ, G6DP, CPT1 and PPARβ/δ (p ≤ 0.0001), but no effect was observed with HX2 and ACCAα (p ≥ 0.05). Conclusions: Our results suggest that differentiated cells exhibit a typical cell lineage metabolism and higher efficiencies both in anabolism and catabolism.

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“…Gillis et al (2004) reported that over 86% of the CLA c-9,t-11 in beef tissue originated from the desaturation of 18:1t-11, based on duodenal flows of 18:1t-11 and CLA c-9,t-11 found within adipose tissue. Presently, there was a similar pattern between the concentration of 18:1t-11 and CLA c-9,t-11 in the muscles, although this was not always statistically evident.…”

Section: Total Lipidsmentioning

confidence: 99%

Influence of oilseed supplement ranging in n-6/n-3 ratio on fatty acid composition and Δ5-, Δ6-desaturase protein expression in steer muscles

Turner

Mitchell

Duynisveld

et al. 2012

Animal

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This study investigated effects of roasted or extruded oilseed supplementation ranging in n-6/n-3 ratios from 0.3 to 5.0 on the fatty acid composition and expression of delta-5 desaturase (D5d) and D6-desaturase (D6d) protein in commercial steer cheek (m. masseter) and diaphragm (pars costalis diaphragmatis) muscles. In general, the n-6/n-3 ratio of the diet had a subsequent effect on the muscle n-6/n-3 ratio (P , 0.05), with muscle 18:2n-6 and 18:3n-3 content relating to proportion of dietary soya bean and linseed (P , 0.01). Compared with canola, pure linseed and soya bean diets reduced 14:1c-9 and 16:1c-9 (P , 0.05) but increased 18:1t-11 and c-9,t-11 conjugated linoleic acid (CLA) content (P , 0.01). Oilseed processing had a minor influence but extruded oilseeds increase 18:1t-11 and c-9,t-11 CLA compared with roasted (P , 0.05). Polar lipid 18:3n-3 and n-3 long-chain polyunsaturated fatty acid (LC, >20 carbons PUFA) derivative content increased in relation to dietary linseed supplementation in the diaphragm (P , 0.01), whereas only 18:3n-3 was increased in the cheek (P , 0.01). Protein expression did not differ between diets; however, in each muscle the D5d protein expression had a stronger association with the desaturase products rather than the precursors. The relationship between D5d protein expression and the muscle LC n-6/n-3 ratio was negative in both muscles (P , 0.05). The relationship between D6d protein expression and the LC n-6/n-3 ratio was positive in the cheek (P , 0.001) and negative in the diaphragm (P , 0.05). In conclusion, diet n-6/n-3 ratio affected muscle 18:2n-6 and 18:3n-3 deposition, whereas the D5d and D6d protein expression had some influence on the polar lipid LC-PUFA profile. Results reaffirm that processed oilseeds can be used to increase the proportion of fatty acids potentially beneficial for human health, by influencing the formation of LC-PUFA and reducing the n-6/n-3 ratio.

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Effects of supplemental rumen-protected conjugated linoleic acid or corn oil on fatty acid composition of adipose tissues in beef cattle1

Cited by 75 publications

References 33 publications

Meat characteristics of Nellore steers fed whole cottonseed

Meat characteristics of Nellore steers fed whole cottonseed

PPAR Agonists Promote the Differentiation of Porcine Bone Marrow Mesenchymal Stem Cells into the Adipogenic and Myogenic Lineages

Influence of oilseed supplement ranging in n-6/n-3 ratio on fatty acid composition and Δ5-, Δ6-desaturase protein expression in steer muscles

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