Effects of supplementing two levels of magnesium aspartate and transportation stress on pork quality and gene expression of 𝛍-calpain and calpastatin of finishing pigs

Abstract

The objective of this study was to investigate the effects of supplementing swine finishing diets with two levels of magnesium aspartate (MgAsp) and short-term transportation stress on blood parameters, pork quality and the mRNA abundance of p-calpain and calpastatin in muscles of finishing pigs. Thirty-six crossbred finishing pigs (mean BW 90 kg) were assigned randomly to 0, 1000, or 2000 mg supplemental Mg from MgAsp per kg of diet for five days before slaughter. Then six pigs from each dietary treatment were subjected either to no transportation stress (NTS) or 2 h of transportation stress (TS). Transportation stress resulted in higher concentrations (p < 0.01) of serum calcium, glucose and cortisol, lower pH (p < 0.01), higher Warner-Bratzler shear force (WBSF) (p < 0.05) and higher calpastatin mRNA abundance (p = 0.05) of longissimus muscle (LM) compared with NTS treatments. Supplementation of MgAsp in TS treatments increased serum Mg concentration (p < 0.05) at 2000 mg of Mg/kg, reduced drip loss (p < 0.05) and improved pork quality colour (p < 0.05) at 2000 mg of Mg/kg, and decreased 1-day and 3-day WBSF (p < 0.05) at 1000 mg of Mg/kg compared with TS treatments fed the control diet. It is concluded that supplementation of MgAsp improves water-holding capacity and pork colour, and alleviates the negative effects of transportation stress on meat tenderness.

Aberrant Histone Acetylation Promotes Mitochondrial Respiratory Suppression in the Brain of Alcoholic Rats

“…A single microRNA can target hundreds of mRNA, affecting a variety of biologic as well as pathologic processes, including those associated with ethanol (Sathyan et al, 2007;Pietrzykowski et al, 2008;Tang et al, 2008;Miranda et al, 2010). A particular microRNA, let-7f, belongs to the let-7 family and has been associated with ethanol and mitochondria.…”

A comparison of the physiochemical features of three tertiary hybrid pigs with and without spent coffee ground supplementation

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The impact of stress on the quality of pork

“…В умовах дрібних присадибних господарств населення дія стресового фактору, очевидно, є меншою у зв'язку з відсутністю потреби транспортувати тварин на далекі відстані і звичними умовами утримання перед забоєм за винятком голодної витримки впродовж 20 год перед забоєм. Дослідники неодноразово вказують на транспортування тварин як вагомий стрес-фактор та розробляють методи зниження його негативного впливу, зокрема вивчають вплив тривалості їх транспортування без зупинки на якість м'яса [3,11], умови мікроклімату за транспортування та передзабійної витримки [7], розташування свиней у транспорті [9] тощо. Окрім цього, зростанню стресу сприяє скупчення тварин перед забоєм в одному приміщенні.…”