Effects of supporting and buffer electrolytes (NaCl, CH3COOH and NH4OH) on the diffusion of BSA in porous media

Hu, Xijun; Duong, D.; Qian, Yu

doi:10.1016/0009-2509(92)80209-u

Cited by 24 publications

(14 citation statements)

References 25 publications

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“…Van Brocklin and David (28) and Hu et al (29) have studied systems involving a charged surface in contact with an electrolytic solution, but they (28,29) did not consider the existence and functioning of an electrical double layer because they used Nernst-Planck models (28,29), which require that both the space charge density, ρ cd , and the current density, i, are equal to zero (ρ cd = 0 and i = 0) everywhere in space for all times; but the requirements of these Nernst-Planck models (28,29) do not satisfy the physical fact that in a system involving a charged surface in contact with an electrolytic solution there will always exist (27) an electrical double layer which has physical properties, as described in items (i)-(iii) above, that are not accounted for in the Nernst-Planck models (28,29). Therefore, the results presented by van Brocklin and David (28) and Hu et al (29) have been obtained from models that do not properly represent the physics of the systems that they (28,29) intended to study.…”

Section: Introductionmentioning

confidence: 99%

The Interplay of Diffusional and Electrophoretic Transport Mechanisms of Charged Solutes in the Liquid Film Surrounding Charged Nonporous Adsorbent Particles Employed in Finite Bath Adsorption Systems

Grimes

Liapis

2002

Journal of Colloid and Interface Science

118

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Section: Introductionmentioning

confidence: 99%

The Interplay of Diffusional and Electrophoretic Transport Mechanisms of Charged Solutes in the Liquid Film Surrounding Charged Nonporous Adsorbent Particles Employed in Finite Bath Adsorption Systems

Grimes

Liapis

2002

Journal of Colloid and Interface Science

118

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“…Same as the model we used for the calculation of insulin adsorption kinetics, 6 we assume that at a relatively high buffer concentration, the diffusion potential generated in the transport process can be neglected, 19 and the effect of the membrane pore restriction is included in the effective diffusion coefficient D, the diffusion of insulin within the membrane (x direction) can then be described by the Fick's second law…”

Section: Diffusion Modelmentioning

confidence: 99%

Insulin transport across porous charged membranes: Effect of the electrostatic interaction

Zhang¹,

Matsumoto²,

Saito³

et al. 2009

Biotechnology Progress

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Insulin transport phenomena across a series of porous charged membranes were studied at two pH conditions (pH 3.3 and pH 7.4) in this article. The membranes were prepared by pore-surface modification of porous poly(acrylonitrile) (PAN) membranes by grafting with weak acidic and basic functional groups. The insulin partition coefficient K between the membrane and solution was estimated from the equilibrium adsorption amount in the batch adsorption experiment. The insulin effective diffusion coefficient D inside the membrane was determined as a fitting parameter by matching the diffusion model with the experimental data of the diffusion measurement. Both K and D correlated well with the charge properties of the insulin and membrane: when the insulin and membrane carried opposite net charge, the partition coefficient showed relatively larger values, while the effective diffusion coefficient was reduced. The insulin permeability coefficient P obtained from the experimental results agreed with that estimated from the partition coefficient and effective diffusion coefficient. These results suggested that the combined effects of the solubility and diffusivity on the permeability coefficient complicated the relationship between the permeability and the charge properties of the insulin and membrane. Additionally, our calculation supported that insulin permeability was reduced by the boundary layer between the membrane and solution.

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“…Suppose that the diffusion potential generated in the transport process can be neglected at a high buffer concentration, 26 and the effect of the membrane pore restriction is included in the effective diffusion coefficient D, the diffusion of insulin within the membrane can then be described by the diffusion model through a plane sheet 14 as…”

Section: Adsorption Modelmentioning

confidence: 99%

Insulin adsorption into porous charged membranes: Effect of the electrostatic interaction

Zhang

Tanioka

Saito

et al. 2009

Biotechnology Progress

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Insulin adsorption into a series of porous charged membranes was investigated by batch adsorption experiments, and the experimental results were analyzed by the homogeneous diffusion model. The membranes used in this study were prepared by pore-surface modification of porous poly(acrylonitrile) (PAN) membranes by grafting with weak acidic and basic functional groups. The amount of insulin adsorbed into the membrane was determined from the material balance of insulin. The insulin partition coefficient K between the membrane and solution was estimated from the equilibrium adsorption amount, and the effective diffusion coefficient D was estimated by matching the model with the experimental data as a fitting parameter. The dependence of K and D on the charge properties of the insulin and membrane is observed and discussed. The partition coefficient K increased when the insulin and the membrane carried opposite charges, on the other hand, the effective diffusion coefficient D was reduced. These results indicate that the electrostatic interaction between the insulin and the membranes played an important role in the insulin adsorption.

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Effects of supporting and buffer electrolytes (NaCl, CH3COOH and NH4OH) on the diffusion of BSA in porous media

Cited by 24 publications

References 25 publications

The Interplay of Diffusional and Electrophoretic Transport Mechanisms of Charged Solutes in the Liquid Film Surrounding Charged Nonporous Adsorbent Particles Employed in Finite Bath Adsorption Systems

The Interplay of Diffusional and Electrophoretic Transport Mechanisms of Charged Solutes in the Liquid Film Surrounding Charged Nonporous Adsorbent Particles Employed in Finite Bath Adsorption Systems

Insulin transport across porous charged membranes: Effect of the electrostatic interaction

Insulin adsorption into porous charged membranes: Effect of the electrostatic interaction

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