Effects of surface ligand density on lipid-monolayer-mediated 2D assembly of proteins

Fukuto, Masafumi; Wang, Suntao; Lohr, Matthew; Kewalramani, Sumit; Yang, Lin

doi:10.1039/b917139h

Cited by 15 publications

(12 citation statements)

References 49 publications

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“…The assembled structure was characterized by in situ grazing-incidence small-angle X-ray scattering (GISAXS) and X-ray reflectivity (XR). 28,32 The nature of the observed 2D crystals was examined further with the aid of numerical analysis of 2D packing based on model TYMV-shaped particles.…”

Section: Methodsmentioning

confidence: 99%

Crystallization, structural diversity and anisotropy effects in 2D arrays of icosahedral viruses

et al. 2013

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We investigate two-dimensional (2D) assembly of the icosahedral turnip yellow mosaic virus (TYMV) under cationic lipid monolayers at the aqueous solution–vapor interface. The 2D crystallization of TYMV has been achieved by enhancing electrostatically induced interfacial adsorption, an approach recently demonstrated for another virus. In situ X-ray scattering reveals two close-packed 2D crystalline phases of TYMV that are distinct from the previously reported hexagonal and centered square ( 2×2) arrays of TYMV. One of the newly observed phases arises from either a dimeric double-square (2 × 1) or tetrameric square (2 × 2) unit cell. The other is a rhombic crystal with a lattice angle of 80°. The two observed crystal phases are substantially less dense (by over10%) than a 2D lattice of TYMV could be according to its known size and shape, indicating that local anisotropic interparticle interactions play a key role in stabilizing these crystals. TYMV’s anisotropy attributes and numerical analysis of 2D arrays of virus-shaped particles are used to derive a model for the rhombic crystal in which the particle orientation is consistent with the electrostatic lipid–TYMV attraction and the interparticle contacts exhibit steric complementarity. The interplay between particle anisotropy and packing is contrasted between the rhombic crystal model and the square ( 2×2) crystal. This study highlights how the high symmetry and subtle asphericity of icosahedral particles enrich the variety and complexity of ordered 2D structures that can be generated through self-assembly.

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Section: Methodsmentioning

confidence: 99%

Crystallization, structural diversity and anisotropy effects in 2D arrays of icosahedral viruses

et al. 2013

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“…One major challenge is to probe both local nanoscale structure and mesoscale heterogeneities, such as phase-coexistence and domain morphologies, which are common in twodimensional (2D) assemblies of nano-objects at liquid interfaces (Kim et al, 2001;Tao et al, 2008;Wang et al, 1997;Ratanabanangkoon & Gast, 2003;Fukuto et al, 2010Fukuto et al, , 2013Kewalramani et al, 2011;Stanley et al, 2015). The established X-ray methods for characterizing liquid-surface structures, i.e.…”

Section: Introductionmentioning

confidence: 99%

Transmission X-ray scattering as a probe for complex liquid-surface structures

Fukuto

Yang

Nykypanchuk

et al. 2016

J Synchrotron Radiat

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The need for functional materials calls for increasing complexity in self-assembly systems. As a result, the ability to probe both local structure and heterogeneities, such as phase-coexistence and domain morphologies, has become increasingly important to controlling self-assembly processes, including those at liquid surfaces. The traditional X-ray scattering methods for liquid surfaces, such as specular reflectivity and grazing-incidence diffraction, are not well suited to spatially resolving lateral heterogeneities due to large illuminated footprint. A possible alternative approach is to use scanning transmission X-ray scattering to simultaneously probe local intermolecular structures and heterogeneous domain morphologies on liquid surfaces. To test the feasibility of this approach, transmission small- and wide-angle X-ray scattering (TSAXS/TWAXS) studies of Langmuir films formed on water meniscus against a vertically immersed hydrophilic Si substrate were recently carried out. First-order diffraction rings were observed in TSAXS patterns from a monolayer of hexagonally packed gold nanoparticles and in TWAXS patterns from a monolayer of fluorinated fatty acids, both as a Langmuir monolayer on water meniscus and as a Langmuir-Blodgett monolayer on the substrate. The patterns taken at multiple spots have been analyzed to extract the shape of the meniscus surface and the ordered-monolayer coverage as a function of spot position. These results, together with continual improvement in the brightness and spot size of X-ray beams available at synchrotron facilities, support the possibility of using scanning-probe TSAXS/TWAXS to characterize heterogeneous structures at liquid surfaces.

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“…43 The X-ray wavelengths used were l ¼ 0.855 A (X9), 1.239 A (X21), and 1.517 A (X22B). For GISAXS, a microfocusing mirror was used to set the incident angle to a z 0.7a c , where a c is the critical angle for the air-water interface (a c ¼ 0.…”

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confidence: 99%

Systematic approach to electrostatically induced 2D crystallization of nanoparticles at liquid interfaces

et al. 2011

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We report an experimental demonstration of a strategy for inducing two-dimensional (2D) crystallization of charged nanoparticles on oppositely charged fluid interfaces. This strategy aims to maximize the interfacial adsorption of nanoparticles, and hence their lateral packing density, by utilizing a combination of weakly charged particles and a high surface charge density on the planar interface. In order to test this approach, we investigated the assembly of cowpea mosaic virus (CPMV) on positively charged lipid monolayers at the aqueous solution surface, by means of in situ X-ray scattering measurements at the liquid-vapor interface. The assembly was studied as a function of the solution pH, which was used to vary the charge on CPMV, and of the mole fraction of the cationic lipid in the binary lipid monolayer, which set the interface charge density. The 2D crystallization of CPMV occurred in a narrow pH range just above the particle's isoelectric point, where the particle charge was weakly negative, and only when the cationic-lipid fraction in the monolayer exceeded a threshold. The observed 2D crystals exhibited nearly the same packing density as the densest lattice plane within the known 3D crystals of CPMV. The above electrostatic approach of maximizing interfacial adsorption may provide an efficient route to the crystallization of nanoparticles at aqueous interfaces.

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Effects of surface ligand density on lipid-monolayer-mediated 2D assembly of proteins

Cited by 15 publications

References 49 publications

Crystallization, structural diversity and anisotropy effects in 2D arrays of icosahedral viruses

Crystallization, structural diversity and anisotropy effects in 2D arrays of icosahedral viruses

Transmission X-ray scattering as a probe for complex liquid-surface structures

Systematic approach to electrostatically induced 2D crystallization of nanoparticles at liquid interfaces

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