Effects of surface microtopography of titanium disks on cell proliferation and differentiation of osteoblast-like cells isolated from rat calvariae

Yokose, Satoshi; Klokkevold, Perry R.; Takei, Henry H.; Kadokura, Hiroshi; Kikui, Tetsuya; Hibino, Y.; Shigeta, H.; Nakajima, Hiroshi; Kawazu, Hiroshi

doi:10.4012/dmj.2017-036

Cited by 9 publications

(8 citation statements)

References 23 publications

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“…The results seemingly indicate that adding roughness either has no affect or a slightly negative affect on osteoblast activity in contrast to past studies that assert that increasing roughness of titanium substrate enhanced cell proliferation or differentiation 15,29‐31 . However, many of these studies produce roughness via subtractive manufacturing, often using acid etching techniques which produce roughness on a smaller scale than was able to be produced in this study using additive manufacturing.…”

Section: Discussioncontrasting

confidence: 73%

“…By focusing on surface roughness, this study evaluates the effect of surface roughness while minimizing confounding variables. In vitro studies have demonstrated that increasing the surface roughness of 3D printed titanium discs with acid etching between R a values of 0.29–3.26 μm significantly increased proliferation and activity of osteoblasts 15 . Micro and nano roughness is theorized to impact osseointegration by providing local asperities for osteoblast attachment, or by influencing the adsorption of proteins on the surface, inducing the expression of certain integrin, such as, α 2 β 1 , which in turn directly regulates osteoblast differentiation 16‐18 .…”

Section: Introductionmentioning

confidence: 99%

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Effect of surface topography on in vitro osteoblast function and mechanical performance of 3D printed titanium

Abar

Kelly

Pham

et al. 2021

J Biomedical Materials Res

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Critical‐sized defects remain a significant challenge in orthopaedics. 3D printed scaffolds are a promising treatment but are still limited due to inconsistent osseous integration. The goal of the study is to understand how changing the surface roughness of 3D printed titanium either by surface treatment or artificially printing rough topography impacts the mechanical and biological properties of 3D printed titanium. Titanium tensile samples and discs were printed via laser powder bed fusion. Roughness was manipulated by post‐processing printed samples or by directly printing rough features. Experimental groups in order of increasing surface roughness were Polished, Blasted, As Built, Sprouts, and Rough Sprouts. Tensile behavior of samples showed reduced strength with increasing surface roughness. MC3T3 pre‐osteoblasts were seeded on discs and analyzed for cellular proliferation, differentiation, and matrix deposition at 0, 2, and 4 weeks. Printing roughness diminished mechanical properties such as tensile strength and ductility without clear benefit to cell growth. Roughness features were printed on mesoscale, unlike samples in literature in which roughness on microscale demonstrated an increase in cell activity. The data suggest that printing artificial roughness on titanium scaffold is not an effective strategy to promote osseous integration.

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Section: Discussioncontrasting

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Effect of surface topography on in vitro osteoblast function and mechanical performance of 3D printed titanium

Abar

Kelly

Pham

et al. 2021

J Biomedical Materials Res

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“…Blatt et al concluded in their study of early cellular response that the different surfaces of implants influence expression patterns in terms of proliferation, adherence, and differentiation in early osteoblastic maturation [51,52]. With regard to the surface obtained from the combination of blasting and acid passivation, several authors have suggested that when greater roughness is obtained, greater cell adhesion, and acceleration occurs in early osseointegration (first week) [53,54].…”

Section: Discussionmentioning

confidence: 99%

Superficial Characteristics of Titanium after Treatment of Chorreated Surface, Passive Acid, and Decontamination with Argon Plasma

Rizo-Gorrita

Luna-Oliva

Serrera-Figallo

et al. 2018

JFB

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(1) Background. Titanium is characterized by its biocompatibility, resistance to maximum stress, and fatigue and non-toxicity. The composition, surface structure, and roughness of titanium have a key and direct influence on the osseointegration processes when it is used in the form of dental implants. The objective of the present study is to characterize, at chemical, superficial, and biological levels, the result of the application of the sandblasted with large-grit and acid-etched (SLA) treatment consisting of coarse-grained and double-passivated acid blasting with subsequent decontamination with argon plasma on the surface of titanium implants type IV. (2) Methods. Four Oxtein® dental implants (Zaragoza, Spain) were investigated with the following coding: Code L63713T (titanium grade IV, 3.75 mm in diameter, and 13 mm in length). The surface of the implants was SLA type obtained from coarse-grained, double passivated acid, and decontaminated with argon plasma. The samples were in their sealed packages and were opened in our laboratory. The X-ray photoelectron spectroscopy (XPS) technique was used to characterize the chemical composition of the surface, and the scanning electronic microscope (SEM) technique was used to perform topographic surface evaluation. Cell cultures were also performed on both surfaces. (3) Results. The superficial chemical analysis of the studied samples presented the following components, approximately, expressed in atomic percentage: O: 39%; Ti: 18%; C: 39%; N: 2%; and Si: 1%. In the same way, the topographic analysis values were obtained in the evaluated roughness parameters: Ra: 1.5 μm ± 0.02%; Rq: 1.31 μm ± 0.33; Rz: 8.98 μm ± 0.73; Rp: 5.12 μm ± 0.48; Rv: 3.76 μm ± 0.51; and Rc: 4.92 μm ± 0.24. At a biological level, the expression of osteocalcin was higher (p < 0.05) on the micro-rough surface compared to that machined at 48 and 96 h of culture. (4) Conclusions. The data obtained in our study indicate that the total carbon content, the relative concentration of titanium, and the roughness of the treatment performed on the implants are in agreement with those found in the literature. Further, the roughness of the treatment performed on the implants throws a spongy, three-dimensional surface suitable for bone growth on it. The biological results found are compatible with the clinical use of the surface tested.

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“…In addition to the roughness, other physical properties such as hydrophilicity also affect cell adhesion and growth [31,32]. Surface hydrophilicity is conducive to cellular attachment and proliferation on the surface [33].…”

Section: Discussionmentioning

confidence: 99%

Biofunctionalization of Microgroove Surfaces with Antibacterial Nanocoatings

Lai¹,

Chen

et al. 2020

BioMed Research International

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Objectives. To investigate the physical properties of the modified microgroove (MG) and antibacterial nanocoated surfaces. In addition, the biological interactions of the modified surfaces with human gingival fibroblasts (HGFs) and the antibacterial activity of the surfaces against Porphyromonas gingivalis were studied. Methods. The titanium nitride (TiN) and silver (Ag) coatings were deposited onto the smooth and MG surfaces using magnetron sputtering. A smooth titanium surface (Ti-S) was used as the control. The physicochemical properties including surface morphology, roughness, and hydrophilicity were characterized using scanning electron microscopy, atomic force microscopy, and an optical contact angle analyzer. The “contact guidance” morphology was assessed using confocal laser scanning microscopy. Cell proliferation was analyzed using the Cell Counting Kit-8 assay. The expression level of the main focal adhesion-related structural protein vinculin was compared using quantitative reverse transcription PCR and Western blotting. The antibacterial activity against P. gingivalis was evaluated using the LIVE/DEAD BacLight™ Bacterial Viability Kit. Results. The Ag and TiN antibacterial nanocoatings were successfully deposited onto the smooth and MG surfaces using magnetron sputtering technology. TiN coating on a grooved surface (TiN-MG) resulted in less nanoroughness and greater surface hydrophilicity than Ag coating on a smooth surface (Ag-S), which was more hydrophobic. Cell proliferation and expression of vinculin were higher on the TiN-MG surface than on the Ag-coated surfaces. Ag-coated surfaces showed the strongest antibacterial activity, followed by TiN-coated surfaces. Conclusion. Nano-Ag coating resulted in good antimicrobial activity; however, the biocompatibility was questionable. TiN nanocoating on an MG surface showed antibacterial properties with an optimal biocompatibility and maintained the “contact guidance” effects for HGFs.

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Effects of surface microtopography of titanium disks on cell proliferation and differentiation of osteoblast-like cells isolated from rat calvariae

Cited by 9 publications

References 23 publications

Effect of surface topography on in vitro osteoblast function and mechanical performance of 3D printed titanium

Effect of surface topography on in vitro osteoblast function and mechanical performance of 3D printed titanium

Superficial Characteristics of Titanium after Treatment of Chorreated Surface, Passive Acid, and Decontamination with Argon Plasma

Biofunctionalization of Microgroove Surfaces with Antibacterial Nanocoatings

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