Effects of Suspended Particulates on the Frequency of Transduction among Pseudomonas aeruginosa in a Freshwater Environment

Ripp, Steven; Miller, Robert V.

doi:10.1128/aem.61.4.1214-1219.1995

Cited by 49 publications

(26 citation statements)

References 22 publications

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“…In the case of E. coli and somatic coliphages the presence of both mormorillonite (Wiggins and Alexander 1985) and bentonite (Muniesa and Jofre 2004) had been reported to increase the 'joint replication threshold' required for phage replication. This information is not in agreement with some observation reported for phages of Pseudomonas (Ripp and Miller 1995), though again it should be kept in mind again that water is the natural habitat of Pseudomonas.…”

Section: Presence Of Suspended Particulates and Nonsusceptible Bacteriacontrasting

confidence: 91%

Is the replication of somatic coliphages in water environments significant?

Jofre

2009

Journal of Applied Microbiology

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Summary Somatic coliphages are amid several groups of bacteriophages that have been suggested as indicators in water quality assessment. One of the limitations frequently endorsed to somatic coliphages as indicators is that they can replicate in the water environment. This review intends to evaluate the significance of this potential replication. In view of: the threshold densities of somatic coliphages and host bacteria needed for productive infection to occur, the densities of both host cells supporting somatic coliphages replication and these phages in water environments, and the poor contribution of lysogenic induction to the free somatic coliphage numbers in water, it can be concluded that replication of somatic coliphages in waters is very unlikely. Consequently, the contribution of replication in the environment of somatic coliphages is expected to have a non‐noticeable influence on the numbers of somatic coliphages detected in water environments. Thus, the replication in the environment should not be argued as a limitation to the use of somatic coliphages as indicators.

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Section: Presence Of Suspended Particulates and Nonsusceptible Bacteriacontrasting

confidence: 91%

Is the replication of somatic coliphages in water environments significant?

Jofre

2009

Journal of Applied Microbiology

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“…11), may have competed with the phage for adsorption sites on aggregates of the clays. The results of these and other studies suggest that clays in the environment may be important as a temporary resting place for genetic material (`environmental chaperone') (Thomas, 1996) that can persist and remain undetected (`cryptic') until an appropriate host is infected (Stotzky, 1989;Stotzky et al, 1991;Straub et al, 1992;Ripp and Miller, 1995;Yin and Stotzky, 1997). Therefore, clay-associated phages could have an important role in maintaining a potential for transduction in natural environments, similar to that of phage lysogeny (Saye et al, 1987;Stotzky, 1989;Marsh and Wellington, 1994).…”

Section: Transduction By Clay±phage Complexes and Interactions Of Thementioning

confidence: 90%

Interaction between bacteriophage PBS1 and clay minerals and transduction of Bacillus subtilis by clay–phage complexes

Vettori

Stotzky

Yoder

et al. 1999

Environmental Microbiology

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Bacteriophage PBS1 of Bacillus subtilis was rapidly adsorbed on montmorillonite (M) and kaolinite (K), and adsorption was maximal after 30min on both clays. There was no correlation between adsorption and the cation exchange capacity of the clays. Studies with sodium metaphosphate (a polyanion that interacts with positively charged sites on clay) indicated that positively charged sites on K were primarily responsible for the adsorption of the phage, whereas other mechanisms appeared to be involved in adsorption of the phage on M. X-ray diffraction and electron microscopic analyses showed that the phage partially intercalated M. Survival of the phage was increased by adsorption on the clays, and adsorbed phage maintained its ability to transduce bacterial cells for at least 30 days (the longest time studied) after the preparation of the clay-phage complexes. Electron microscopic observations indicated that transduction by the clay-phage complexes was primarily the result of the phage detaching from the clays in the presence of host cells.

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“…Transduction rates have not been often determined in natural environments and were mainly investigated as tools for studying bacterial genetics or for the risk assessment of the spread of genes from genetically engineered microorganisms. Most studies from lake water were performed by Miller and co-workers [281,284,450,451] by using microcosms and P. aeruginosa as system. However, transduction was also studied in a number of microcosms obtained from soils and the sea [452][453][454][455].…”

Section: Horizontal Gene Transfermentioning

confidence: 99%

Ecology of prokaryotic viruses

2004

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The finding that total viral abundance is higher than total prokaryotic abundance and that a significant fraction of the prokaryotic community is infected with phages in aquatic systems has stimulated research on the ecology of prokaryotic viruses and their role in ecosystems. This review treats the ecology of prokaryotic viruses ('phages') in marine, freshwater and soil systems from a 'virus point of view'. The abundance of viruses varies strongly in different environments and is related to bacterial abundance or activity suggesting that the majority of the viruses found in the environment are typically phages. Data on phage diversity are sparse but indicate that phages are extremely diverse in natural systems. Lytic phages are predators of prokaryotes, whereas lysogenic and chronic infections represent a parasitic interaction. Some forms of lysogeny might be described best as mutualism. The little existing ecological data on phage populations indicate a large variety of environmental niches and survival strategies. The host cell is the main resource for phages and the resource quality, i.e., the metabolic state of the host cell, is a critical factor in all steps of the phage life cycle. Virus-induced mortality of prokaryotes varies strongly on a temporal and spatial scale and shows that phages can be important predators of bacterioplankton. This mortality and the release of cell lysis products into the environment can strongly influence microbial food web processes and biogeochemical cycles. Phages can also affect host diversity, e.g., by 'killing the winner' and keeping in check competitively dominant species or populations. Moreover, they mediate gene transfer between prokaryotes, but this remains largely unknown in the environment. Genomics or proteomics are providing us now with powerful tools in phage ecology, but final testing will have to be performed in the environment.

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Effects of Suspended Particulates on the Frequency of Transduction among Pseudomonas aeruginosa in a Freshwater Environment

Cited by 49 publications

References 22 publications

Is the replication of somatic coliphages in water environments significant?

Is the replication of somatic coliphages in water environments significant?

Interaction between bacteriophage PBS1 and clay minerals and transduction of Bacillus subtilis by clay–phage complexes

Ecology of prokaryotic viruses

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