Effects of systemic transplantation of adipose tissue‐derived stem cells on olfactory epithelium regeneration

Kim, Yong Min; Choi, Young Seok; Choi, Jin Woong; Park, Yong-Ho; Koo, Bon Seok; Roh, Hwan‐Jung; Rha, Ki-Sang

doi:10.1002/lary.20187

Cited by 23 publications

(12 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Compared to the control group, the BMSC transplantation group had increased NGF expression. The expression began to increase 1 week after transplantation and peaked 3-5 weeks after transplantation [15,18,19]. As in our assessment system, measuring the thickness of the epithelium and performing nerve sheath analysis seem reasonable.…”

Section: Discussionmentioning

confidence: 96%

Engraftment and regenerative effects of bone marrow stromal cell transplantation on damaged rat olfactory mucosa

Kwon

Park

et al. 2016

Eur Arch Otorhinolaryngol

View full text Add to dashboard Cite

To develop a new therapeutic method to treat olfactory deficits, we investigated the engraftment and regenerative effects of transplanted bone marrow stromal cells (BMSCs) on damaged rat olfactory mucosa. To induce olfactory nerve degeneration, one side of the olfactory mucosa of Sprague-Dawley rats was damaged via Triton X-100 irrigation. Phosphate-buffered saline containing syngeneic BMSCs was injected into the olfactory mucosa for transplantation. PKH fluorescent cell dye labeling of BMSCs was used to monitor the transplanted cells. After transplantation of BMSCs, the thickness and regeneration of olfactory mucosa were analyzed using hematoxylin-eosin (H&E) staining. S100 immunohistochemical staining was used to measure nerve sheath regeneration. The increase in NGF (nerve growth factor) level in the olfactory mucosa was measured by Western blot analysis. Transplanted bone marrow stromal cells were engrafted to the lamia propria of damaged mucosa. The mean time for normalization of thickness and morphological recovery of the olfactory mucosa was 4 weeks in the therapeutic group and 9 weeks in the control group. S100 immunoreactivity was higher on the BMSC-treated side than on the control side. During regeneration, the expression of NGF increased in the olfactory mucosa of the experimental group. Based on these results, BMSC transplantation accelerated regeneration of olfactory mucosa damaged by Triton X-100, and NGF may be essential to this regenerative process.

show abstract

Section: Discussionmentioning

confidence: 96%

Engraftment and regenerative effects of bone marrow stromal cell transplantation on damaged rat olfactory mucosa

Kwon

Park

et al. 2016

Eur Arch Otorhinolaryngol

View full text Add to dashboard Cite

show abstract

“…Human cord blood CD133-positive stem cells were intravenously injected to nod-scid mice and this was shown to provide conditions for regeneration of the olfactory neuroepithelium after permanent damage was induced by dichlobenil (16). Adipose tissue-derived stem cells were also intravenously injected to rats after their olfactory nerve was transected (17). However, the olfactory nerve transaction model is less likely to be appropriate for stem cell research.…”

Section: Discussionmentioning

confidence: 99%

Transplantation of Neural Stem Cells in Anosmic Mice

Jeon

Seo

et al. 2010

Clin Exp Otorhinolaryngol

View full text Add to dashboard Cite

ObjectivesTreating olfactory dysfunction is a challenge for physicians. One of the therapeutic options could be transplantation of stem cells. In this study, neural stem cells were transplanted into anosmic mice.MethodsNeural stem cells were generated from the olfactory bulb of green fluorescent protein (GFP)-transgenic C57BL6 mice. Anosmia were induced by injection of intraperitoneal 3-methylindole. The neural stem cells were transplanted transnasally on the next day. The olfactory function was evaluated by a food-finding test once a week. The olfactory neuroepithelium was harvested for histologic examination and protein analysis at 4 weeks.ResultsTwenty-five percent (6/24) of the control mice that were not transplanted with neural stem cells survived at 4 weeks while 67% (8/12) of the transplanted mice survived (P=0.029). The food finding test showed that the transplanted mice resumed finding food at 3 weeks while the control mice resumed finding food at 4 weeks. GFP-positive cells were observed in the olfactory neuroepithelium of the transplanted mice. Western blotting revealed that the olfactory marker protein expression was significantly lower in the control mice than that in the transplanted mice.ConclusionThis study demonstrated that improvement of mouse survival was achieved and recovery of olfactory function was promoted by transnasal transplantation of neural stem cells in the anosmic mouse model. These results indicate that stem cells might be one of the future modalities for treating olfactory impairment.

show abstract

“…Numerous studies in rat [70] , pig [71] and human models [72] have demonstrated the multipotentiality of head and neck-derived ASC.…”

Section: Profile and Identitymentioning

confidence: 99%

“…In another study, Kim et al showed the potential application of neckderived ASC for the treatment of head trauma-related olfactory dysfunction in terms of regeneration epithelium [70] . Olfactory epithelial degeneration was first induced in a rat model through unilateral transection of the olfactory nerve; regeneration of olfactory epithelium was then observed following systemic transplantation of neck-derived ASC [70] .…”

Section: Profile and Identitymentioning

confidence: 99%

“…Olfactory epithelial degeneration was first induced in a rat model through unilateral transection of the olfactory nerve; regeneration of olfactory epithelium was then observed following systemic transplantation of neck-derived ASC [70] . Derived from the head and neck, neural crest cells consist of heterogeneous progenitor mesenchymal cells which can give rise to craniofacial cartilage, bone, dermis, adipose tissue and vascular smooth muscle cells [74] .…”

Section: Profile and Identitymentioning

confidence: 99%

See 1 more Smart Citation

Craniomaxillofacial Sources of Mesenchymal Stem Cells: A Brief Review

Nguyen

James

et al. 2015

International Journal of Orthopaedics

View full text Add to dashboard Cite

Tissue specific mesenchymal stem cells are of great scientific interest across diverse fields in stem cell biology and medicine. Sources of mesenchymal stem cells (MSC) in the craniomaxillofacial skeleton are numerous and are of significant interest to craniofacial and oral surgeons, among other medical and dental specialties. Mesenchymal stem cells are defined by their characteristic cell surface marker expression, multipotentiality and ability for self-renewal. Bone marrow derived MSC (BMSC) are most commonly studied, but are of low abundance in the craniomaxillofacial (CMF) skeleton. Alternative CMF sources of MSC are diverse and include dental pulpal stem cells (DPSC), periodontal ligament stem cells (PDLSC), suture-derived mesenchymal stromal cells (SMC), gingival mesenchymal stem cells (GMSC)and adipose-derived MSC (ASC). This brief review will introduce various MSC sources derived from the head and neck, along with a discussion of their identity, characteristics and results of preclinical studies in tissue engineering.

show abstract

Effects of systemic transplantation of adipose tissue‐derived stem cells on olfactory epithelium regeneration

Abstract: Our findings suggest the feasibility of ADSC transplantation as a treatment for head trauma-related olfactory dysfunction.

Cited by 23 publications

References 20 publications

Engraftment and regenerative effects of bone marrow stromal cell transplantation on damaged rat olfactory mucosa

Engraftment and regenerative effects of bone marrow stromal cell transplantation on damaged rat olfactory mucosa

Transplantation of Neural Stem Cells in Anosmic Mice

Craniomaxillofacial Sources of Mesenchymal Stem Cells: A Brief Review

Contact Info

Product

Resources

About