Effects of tannins on Chinese hamster cell line B14

Łabieniec, Magdalena; Gabryelak, Teresa

doi:10.1016/s1383-5718(03)00161-x

Cited by 66 publications

(40 citation statements)

References 23 publications

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“…There are also findings that condensed tannins from grape seeds decrease membrane fluidity in both the hydrophobic and hydrophilic parts of erythrocyte membranes [18]. For hydrolysable tannins, increasing fluidity in the internal region of the lipid bilayer has been shown, but no changes were found on the surface of the membrane of the Chinese hamster cell line B14 [50]. It is known that the binding of tannins to the membrane surface of cells may induce cluster and raft formation [34].…”

Section: Discussionmentioning

confidence: 99%

Stabilization of erythrocytes against oxidative and hypotonic stress by tannins isolated from sumac leaves (Rhus typhina L.) and grape seeds (Vitis vinifera L.)

Olchowik

Lotkowski

Mavlyanov

et al. 2012

Cellular and Molecular Biology Letters

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Erythrocytes are constantly exposed to ROS due to their function in the organism. High tension of oxygen, presence of hemoglobin iron and high concentration of polyunsaturated fatty acids in membrane make erythrocytes especially susceptible to oxidative stress. A comparison of the antioxidant activities of polyphenol-rich plant extracts containing hydrolysable tannins from sumac leaves (Rhus typhina L.) and condensed tannins from grape seeds (Vitis vinifera L.) showed that at the 5-50 µg/ml concentration range they reduced to the same extent hemolysis and glutathione, lipid and hemoglobin oxidation induced by erythrocyte treatment with 400 µM ONOO -or 1 mM HClO. However, extract (condensed tannins) from grape seeds in comparison with extract (hydrolysable tannins) from sumac leaves stabilized erythrocytes in hypotonic NaCl solutions weakly. Our data indicate that both hydrolysable and condensed tannins significantly decrease the fluidity of the surface of erythrocyte membranes but the effect of hydrolysable ones was more profound. In conclusion, our results indicate that extracts from sumac leaves (hydrolysable tannins) and grape seeds (condensed tannins) are very effective protectors against oxidative damage in erythrocytes.

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Section: Discussionmentioning

confidence: 99%

Stabilization of erythrocytes against oxidative and hypotonic stress by tannins isolated from sumac leaves (Rhus typhina L.) and grape seeds (Vitis vinifera L.)

Olchowik

Lotkowski

Mavlyanov

et al. 2012

Cellular and Molecular Biology Letters

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“…Researchers have documented the in vitro cytotoxicity and genotoxicity induced by ellagic and gallic acid (15 - 240 mM concentration range) as measured in culture of B14 cell line [32]. The results of this study showed that tannins could decrease the viability of cells and their cytotoxicity was highest at the concentration of 60 mM.…”

Section: Discussionmentioning

confidence: 77%

Further Pharmacological Evidence Supporting the Development of an Antiulcerogenic Drug Based on Rhizophora mangle L. Aqueous Extract. HPLC Method Proposed for Determinating a Chemical Marker

Perera¹,

Piloto²,

Canelsota³

et al. 2016

OALib

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Rhizophora mangle L. is a vegetal species with high disponibility in Cuba for guarantying economical factibility by drug development. Preview studies showed promissory pharmacological results by the use of this plant in the treatment of gastroduodenal ulcers. The present work involved another evaluation of the active extract important by the development of a new drug. It was evaluated by intestinal absorption in vitro and in situ models and anti-diarrheic activity in rats. The polyphenolic fractions were separated from the aqueous extract and its cytoprotective activity was studied on gastric ulceration induced by ethanol plus hydrochloric acid in rats. Some toxicological studies were carried out. We made a validation of HPLC method by the analysis of one mark in the tablet development. The aqueous extract showed high absorption, and high anti-diarrheic effect. The low-molecular weight polyphenolic fraction showed the highest level of gastric protection. No toxicological signs were obtained by it. HPLC method evidenced linearity, precision, sensitivity and accuracy.

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“…Bu besinlerde bulunan taninler, hidrolize ve kondanse taninler olmak üzere iki sınıfa ayrılırlar (20,21). Tanin içe-ren polifenoller antitümöral, antiviral, antiHIV, lipid peroksidasyon inhibisyonu ve plazmin aktivitesi gibi biyolojik aktivitelere sahiptirler (22). Hidrolize taninlerden olan Tannik asit (TA), çay, yeşil çay, kahve, kırmızı şarap, fındık ve ceviz gibi kabuklu yemiş, meyve ve çoğu bitkide bulunur (21,23,24).…”

Section: Introductionunclassified

Kadmi̇yum Toksi̇si̇tesi̇ Oluşturulan Siçanlarda Tanni̇k Asi̇ti̇n, Ağir Metal Gi̇deri̇mi̇ Ve Bazi Bi̇yoki̇myasal Değerler Üzeri̇ne Etki̇si̇ni̇n Araştirilmasi

Coşan¹,

Dal²,

Soyocak³

et al. 2017

Kocatepe Tıp Dergisi

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ÖZ AMAÇ:Endüstriyel alanda yoğun bir şekilde kullanılan, önemli çevre kirleticilerden biri olan kadmiyum (Cd), günlük hayattaki maruziyetimiz nedeniyle yaşamsal bir takım tehditlere sebep olabilmektedir. Uzun bir yarılanma ömrüne sahip olan Cd, solunum, üriner, kardiovasküler, gastrointestinal, sinir sistemi ve kemiklerde direkt veya dolaylı olarak toksisiteye neden olmaktadır. Vücutta biriken Cd'un osteoporoz, anemi, eozinofili, anozmi ve kronik rinit gibi birçok hastalığa neden olduğu bilinmektedir. Çalışmamızda, flavonoid içeriği oldukça yüksek, ağır metal şe-latlayıcı özelliğe sahip ve antioksidan tannik asidin (TA) Cd toksikasyonundan korunmadaki rolünü araştırmak amaçlandı. GEREÇ VE YÖNTEM:Bu amaçla, 28 adet 200-300 gram ağırlığında sağlıklı 3-4 aylık Spraque-Dawley soyu erkek sıçanlar kullanıldı. Deney hayvanlarına ön tedavi amaçlı intraperitonal (i.p) enjeksiyon ile 5 gün boyunca TA 50mg/kg/gün olarak uygulandıktan sonra, intragastrik (i.g) enjeksiyon tek doz 35mg/ kg CdCl2 uygulandı. BULGULAR:CdCl2 uygulaması ile azalan hemolizat, karaciğer ve böbrek süperoksit dismutaz (SOD) değerlerini TA'nın artırdığı gözlendi. Benzer şekilde artan hemolizat ve karaciğer malondialdehit (MDA) değerlerini TA'nın azalttığı belirlendi. Karaciğer, böbrek, akciğer, testis ve dalakta CdCl2 uygulaması ile artan birikimi azaltma yönünde, TA etkili olmazken, kalpteki birikimi azaltma yönünde TA'nın etkili olduğu analiz edildi. CdCl2 uygulaması ile artan demir birikimini azaltma yönünde böbrek, dalak ve kalpte TA etkili olmazken, karaciğer, akciğer ve testiste etkili olduğu tespit edildi. SONUÇ:Günlük diyette kullanılan yiyecek ve içeceklerin, toksik ajanlara maruz kalma sonucu gelişebilecek bazı hastalıkları erken dönemde önlenmesiyle, bireylerin yaşam kalitesi yük-selebilecektir. Çalışmamızda günlük yaşantımızda tüketilen TA içeren yiyeceklerin, çeşitli şekillerde maruz kaldığımız ağır metallerden korunma üzerine olan etkileri gözlemlendi. Elde edilen sonuçlara göre farklı dokularda farklı sonuçlara ulaşıla-bileceği belirlenirken, genel olarak değerlendirildiğinde, Cd'un zararlı etkilerine ya da en azından bazı dokulardaki birikimini azaltma yönünde TA'nın etkili olabileceği sonucuna ulaşıldı.ANAHTAR KELİMELER: Kadmiyum, Tannik asit, Toksisite, Sıçan ABSTRACT OBJECTIVE: Cadmium (Cd), one of the major environmental pollutants used intensively in the industrial field and could causes a variety of vital threats due to our exposure in our daily lives. Having a long half-life, Cd causes direct or indirect toxicity to respiratory, urinary, cardiovascular, gastrointestinal, nervous system and bones. It is known that Cd that accumulates in the body causes many diseases such as osteoporosis, anemia, eosinophilia, anosmia and chronic rhinitis. In our study, we aimed to investigate the role of tannic acid (TA), an antioxidant substance with a high content of flavonoids and heavy metal chelating agents, in order to investigate its role in protecting from Cd toxicity. MATERIALS AND METHODS:In this study, it was used 28 Spraque-Dawley, 200...

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Effects of tannins on Chinese hamster cell line B14

Cited by 66 publications

References 23 publications

Stabilization of erythrocytes against oxidative and hypotonic stress by tannins isolated from sumac leaves (Rhus typhina L.) and grape seeds (Vitis vinifera L.)

Stabilization of erythrocytes against oxidative and hypotonic stress by tannins isolated from sumac leaves (Rhus typhina L.) and grape seeds (Vitis vinifera L.)

Further Pharmacological Evidence Supporting the Development of an Antiulcerogenic Drug Based on Rhizophora mangle L. Aqueous Extract. HPLC Method Proposed for Determinating a Chemical Marker

Kadmi̇yum Toksi̇si̇tesi̇ Oluşturulan Siçanlarda Tanni̇k Asi̇ti̇n, Ağir Metal Gi̇deri̇mi̇ Ve Bazi Bi̇yoki̇myasal Değerler Üzeri̇ne Etki̇si̇ni̇n Araştirilmasi

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