Effects of target depletion on adult mammalian central neurons: Morphological correlates

Cruz, Rosa R. de la; Pastor, Ángel M.; Delgado-García, J.M.

doi:10.1016/0306-4522(94)90156-2

Cited by 38 publications

(40 citation statements)

References 60 publications

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“…9A). Since numbers of retrogradely labeled motoneurons reflect the extent of motoneuron survival (de la Cruz et al, 1994;Waters et al, 1998;Asahara et al, 1999, Guseva et al, 2009, we conclude that treatment with histone H1 reduces motoneuron death, a characteristic feature of the femoral nerve injury paradigm in mice (Simova et al, 2006). In addition, histone H1 application increased the number of regenerated motoneurons projecting to the appropriate motor nerve branch and thus increased preferential motor reinnervation, which is a characteristic feature of femoral nerve regeneration (Brushart, 1988;Al-Majed et al, 2000;Franz et al, 2005;Vrbova et al, 2009).…”

Section: Histone H1 Promotes Functional Recovery After Femoral Nerve mentioning

confidence: 83%

Functional Role of the Interaction between Polysialic Acid and Extracellular Histone H1

Mishra¹,

Ohe²,

Schulze³

et al. 2010

J. Neurosci.

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Polysialic acid (PSA) is a large and highly negatively charged glycan that plays crucial roles in nervous system development and function in the adult. It has been suggested to facilitate cell migration, neurite outgrowth, and synaptic plasticity because its hydration volume could enhance flexibility of cell interactions. Evidence for receptors of PSA has so far been elusive. We now identified histone H1 as binding partner of PSA via a single-chain variable fragment antibody using an anti-idiotypic approach. Histone H1 directly binds to PSA as shown by ELISA. Surface biotinylation of cultured cerebellar neurons indicated an extracellular localization of histone H1. Immunostaining of live cerebellar neurons and Schwann cells confirmed that an extracellular pool of histone H1 colocalizes with PSA at the cell surface. Histone H1 was also detected in detergent-insoluble synaptosomal membrane subfractions and postsynaptic densities. When applied in vitro, histone H1 stimulated neuritogenesis, process formation and proliferation of Schwann cells, and migration of neural precursor cells via a PSA-dependent mechanism, further indicating that histone H1 is active extracellularly. These in vitro observations suggested an important functional role for the interaction between histone H1 and PSA not only for nervous system development but also for regeneration in the adult. Indeed, histone H1 improved functional recovery, axon regrowth, and precision of reinnervation of the motor branch in adult mice with femoral nerve injury. Our findings encourage investigations on the therapeutic potential of histone H1 in humans.

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Section: Histone H1 Promotes Functional Recovery After Femoral Nerve mentioning

confidence: 83%

Functional Role of the Interaction between Polysialic Acid and Extracellular Histone H1

Mishra¹,

Ohe²,

Schulze³

et al. 2010

J. Neurosci.

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“…In cats, these cells exhibit marked changes in firing properties after target removal, but return to normal after 1 month. This is likely due to the reestablishment of synaptic connections with novel targets within their terminal area (de la Cruz et al, 1994a(de la Cruz et al, ,b, 1996Pastor et al, 2000), although this also occurs with implanted neurons after embryonic graft (Benítez-Temiño et al, 2002;for review, see Benítez-Temiño et al, 2005).…”

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confidence: 96%

Differential regulation of the expression of neurotrophin receptors in rat extraocular motoneurons after lesion

Morcuende

Matarredona

Benítez‐Temiño

et al. 2011

J of Comparative Neurology

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Neurotrophins acting through high-affinity tyrosine kinase receptors (trkA, trkB, and trkC) play a crucial role in regulating survival and maintenance of specific neuronal functions after injury. Adult motoneurons supplying extraocular muscles survive after disconnection from the target, but suffer dramatic changes in morphological and physiological properties, due in part to the loss of their trophic support from the muscle. To investigate the dependence of the adult rat extraocular motoneurons on neurotrophins, we examined trkA, trkB, and trkC mRNA expression after axotomy by in situ hybridization. trkA mRNA expression was detectable at low levels in unlesioned motoneurons, and its expression was downregulated 1 and 3 days after injury. Expression of trkB and trkC mRNAs was stronger, and after axotomy a simultaneous, but inverse regulation of both receptors was observed. Thus, whereas a considerable increase in trkB expression was seen about 2 weeks after axotomy, the expression of trkC mRNA had decreased at the same post-lesion period. Injured extraocular motoneurons also experienced an initial induction in expression of calcitonin gene-related peptide and a transient downregulation of cholinergic characteristics, indicating a switch in the phenotype from a transmitter-specific to a regenerative state. These results suggest that specific neurotrophins may contribute differentially to the survival and regenerative responses of extraocular motoneurons after lesion.

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“…The oculomotor system (and more precisely the abducens nucleus) has long been used as a model to study plasticity in the adult nervous system [20,21,22,23,24,25,26,27]. There are several points that make this motor system suitable for this line of research.…”

Section: Extraocular Motoneurons As the Experimental Modelmentioning

confidence: 99%

“…Experiments of toxin injection in the medial rectus muscle of adult animals, which led to motoneuron toxicity and death, were performed to produce the depletion of abducens internuclear neuron target cells. Target-deprived internuclear neurons suffered a process of soma shrinkage, dendritic retraction, and the loss of afferent contacts that, finally, produced changes in their normal firing pattern related to eye velocity and position [20,21]. …”

Section: Extraocular Motoneurons As the Experimental Modelmentioning

confidence: 99%

“…Thus, axotomized abducens motoneurons recovered their normal properties by three months after axotomy, probably due to muscle reinnervation [23]. Abducens internuclear neurons also established new contacts in the oculomotor nucleus after target depletion, which allowed the recovery of firing properties [20,21]. …”

Section: Extraocular Motoneurons As the Experimental Modelmentioning

confidence: 99%

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Functional Diversity of Neurotrophin Actions on the Oculomotor System

Benítez‐Temiño¹,

Carrizosa²,

Morcuende³

et al. 2016

IJMS

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Neurotrophins play a principal role in neuronal survival and differentiation during development, but also in the maintenance of appropriate adult neuronal circuits and phenotypes. In the oculomotor system, we have demonstrated that neurotrophins are key regulators of developing and adult neuronal properties, but with peculiarities depending on each neurotrophin. For instance, the administration of NGF (nerve growth factor), BDNF (brain-derived neurotrophic factor) or NT-3 (neurotrophin-3) protects neonatal extraocular motoneurons from cell death after axotomy, but only NGF and BDNF prevent the downregulation in ChAT (choline acetyltransferase). In the adult, in vivo recordings of axotomized extraocular motoneurons have demonstrated that the delivery of NGF, BDNF or NT-3 recovers different components of the firing discharge activity of these cells, with some particularities in the case of NGF. All neurotrophins have also synaptotrophic activity, although to different degrees. Accordingly, neurotrophins can restore the axotomy-induced alterations acting selectively on different properties of the motoneuron. In this review, we summarize these evidences and discuss them in the context of other motor systems.

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Effects of target depletion on adult mammalian central neurons: Morphological correlates

Cited by 38 publications

References 60 publications

Functional Role of the Interaction between Polysialic Acid and Extracellular Histone H1

Functional Role of the Interaction between Polysialic Acid and Extracellular Histone H1

Differential regulation of the expression of neurotrophin receptors in rat extraocular motoneurons after lesion

Functional Diversity of Neurotrophin Actions on the Oculomotor System

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