Effects of Tebuconazole Application on Soil Microbiota and Enzymes

Baćmaga, Małgorzata; Wyszkowska, Jadwiga; Borowik, Agata; Kucharski, J.

doi:10.3390/molecules27217501

Cited by 10 publications

(7 citation statements)

References 57 publications

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“…TEB is widely used in agriculture and viticulture to control a plethora of fungal diseases (Baćmaga et al 2022 ). We found no specific information about the regulatory limits of TEB; in Europe, it is not listed as a priority substance and thus no environmental quality standards have been defined.…”

Section: Discussionmentioning

confidence: 99%

Novel yeast-based biosensor for environmental monitoring of tebuconazole

Mendes,

Miranda,

Amaral

et al. 2024

Appl Microbiol Biotechnol

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Due to increasing demand for high and stable crop production, human populations are highly dependent on pesticide use for growing and storing food. Environmental monitoring of these agrochemicals is therefore of utmost importance, because of their collateral effects on ecosystem and human health. Even though most current-use analytical methods achieve low detection limits, they require procedures that are too complex and costly for routine monitoring. As such, there has been an increased interest in biosensors as alternative or complementary tools to streamline detection and quantification of environmental contaminants. In this work, we developed a biosensor for environmental monitoring of tebuconazole (TEB), a common agrochemical fungicide. For that purpose, we engineered S. cerevisiae cells with a reporter gene downstream of specific promoters that are expressed after exposure to TEB and characterized the sensitivity and specificity of this model system. After optimization, we found that this easy-to-use biosensor consistently detects TEB at concentrations above 5 μg L−1 and does not respond to realistic environmental concentrations of other tested azoles, suggesting it is specific. We propose the use of this system as a complementary tool in environmental monitoring programs, namely, in high throughput scenarios requiring screening of numerous samples. Key points • A yeast-based biosensor was developed for environmental monitoring of tebuconazole. •The biosensor offers a rapid and easy method for tebuconazole detection ≥ 5 μg L−1. •The biosensor is specific to tebuconazole at environmentally relevant concentrations.

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Section: Discussionmentioning

confidence: 99%

Novel yeast-based biosensor for environmental monitoring of tebuconazole

Mendes,

Miranda,

Amaral

et al. 2024

Appl Microbiol Biotechnol

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“…Some studies have found insignificant effects of Tebuconazole treatment on the bacterial and fungal community structures living in sediments and leaf material [ 18 ]. Bacmaga et al (2022) [ 66 ] found that Tebuconazole did not apparently affect the normal biological characteristics of soils after its use in agriculture. The authors showed that on the contrary, after the application of Tebuconazole, a stimulation of heterotrophic bacteria and fungi was observed, as well as the enzymatic activities of soil, such as those of dehydrogenases, alkaline phosphatase, and beta glucosidase.…”

Section: Resultsmentioning

confidence: 99%

“…Other authors [ 67 , 68 ] have noted a significant decrease in microbial biomass after the use of high doses of Tebuconazole. Toxic concentrations for fish and invertebrates do not seem to have a visible effect on microbial communities associated with sediments or leaf material in microcosm systems [ 18 , 66 , 69 , 70 ]. The influence of Tebuconazole on aquatic microbial communities was moderate, and its effect depended on the particularities of the respective ecosystems [ 71 , 72 ].…”

Section: Resultsmentioning

confidence: 99%

Multiple Assays on Non-Target Organisms to Determine the Risk of Acute Environmental Toxicity in Tebuconazole-Based Fungicides Widely Used in the Black Sea Coastal Area

Tofan

Niță

Nenciu

et al. 2023

Toxics

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The widespread use of Tebuconazole-based fungicides in phytosanitary treatments on a wide range of crops, on the one hand, and the lack of official reports on the amount of fungicide residues in nearby water basins, on the other hand, may lead to uncontrolled and hazardous contamination of water sources used by the resident population, and to serious effects on the environment and public health. Our study explores the acute toxicological risk of this fungicide on various organisms, from bacteria and yeast to fish, using a battery of tests (standardized Toxkit microbiotests and acute semi-static tests). By investigating the interaction between Tebuconazole and bacteria and yeast organisms, we observed that Gram-negative bacteria displayed a strong tolerance for Tebuconazole, while Gram-positive bacteria and yeasts proved to be very sensitive. The fish experiment was conducted on Chelon auratus juveniles exposed to five concentrations of the fungicide Tebustar EW (Tebuconazole, 250 g/L as active substance). After 96 h of exposure, the LC50 for C. auratus was 1.13 mg/L. In the case of the Toxkit microbiotests’ application, the following results were recorded: Spirodela polyrhiza EC50 = 2.204 mg/L (after 72 h exposure), Thamnocephalus platyurus EC50 = 0.115 mg/L (after 24 h), and Daphnia magna EC50 = 2.37 mg/L (after 24–48 h). With the exception of bacteria and yeast, the same response pattern was observed for all non-target species tested; the response range expressed by concentrations causing growth inhibition or mortality was small, ranging between very close values that are quite low, thereby demonstrating the high toxicity of Tebuconazole-based fungicides to the environment.

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“…Annually, 20% of wheat, the world’s most widely grown crop and consumed food, is lost due to pests and diseases, − with fungal, viral, and bacterial pathogens causing the most serious damage. Currently, triazole-based pesticides, which provide protection, treatment, and eradication through their broad-spectrum bactericidal properties, represent the most commonly used class of antibacterial drugs during wheat growth. , Of these, tebuconazole (Teb) is the most widely applied one, − but being water soluble, its effective bacteriostatic time after applications tends to be short. , Thus, it is difficult to achieve long-term control of crop pathogens even by increasing the dosage of Teb. At the same time, large amounts of lost drugs can also result in significant pollution of the aquatic environment. ,, Therefore, as far as wheat production is concerned, it would be important to study new pesticide formulations that not only offer long-lasting effects but also allow an optimum use of Teb.…”

Section: Introductionmentioning

confidence: 99%

“…7,8 Of these, tebuconazole (Teb) is the most widely applied one, 9−12 but being water soluble, its effective bacteriostatic time after applications tends to be short. 13,14 Thus, it is difficult to achieve long-term control of crop pathogens even by increasing the dosage of Teb. At the same time, large amounts of lost drugs can also result in significant pollution of the aquatic environment.…”

Section: Introductionmentioning

confidence: 99%

Tebuconazole-Loaded Mesoporous Silica Nanoparticles Encapsulated with Chitosan and Their Application in Wheat Growth

Dai

Shi³

et al. 2023

ACS Agric. Sci. Technol.

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Controlled-release pesticide formulation exhibiting low phytotoxicity and long-term effectiveness is highly promising. In this work, a novel pH stimuli-responsive pesticide delivery system was established by combining spray drying with ion crosslinking to coat chitosan onto tebuconazole-loaded mesoporous silica nanoparticles (Teb@MSNs@CS). The results demonstrated that the loading capacity of Teb@MSNs@CS for tebuconazole (Teb) was approximately 25.80%. The chitosan attached on the mesoporous silica nanoparticles could extend the release time of Teb both in the solution medium and soil column. Meanwhile, the coated chitosan endowed the pH-responsive release of Teb. The bioactivity survey confirmed that Teb@MSNs@CS exhibited enhanced control of Fusarium graminearum compared to pure Teb. Moreover, Teb@MSNs@CS displayed lower phytotoxicity than both Teb@MSNs and pure Teb during wheat germination and seeding growth experiments. As revealed by the above-mentioned results, the use of responsive carriers as the pesticide-delivery systems can be potentially implemented in wheat growth.

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Effects of Tebuconazole Application on Soil Microbiota and Enzymes

Cited by 10 publications

References 57 publications

Novel yeast-based biosensor for environmental monitoring of tebuconazole

Novel yeast-based biosensor for environmental monitoring of tebuconazole

Multiple Assays on Non-Target Organisms to Determine the Risk of Acute Environmental Toxicity in Tebuconazole-Based Fungicides Widely Used in the Black Sea Coastal Area

Tebuconazole-Loaded Mesoporous Silica Nanoparticles Encapsulated with Chitosan and Their Application in Wheat Growth

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