Effects of Temperature on Biological Activity of Permafrost Microorganisms

Kalyonova, L. F.; Новикова, М. А.; Субботин, А. М.; Bazhin, A. S.

doi:10.1007/s10517-015-2859-z

Cited by 7 publications

(2 citation statements)

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“…The obtained data together with the previously obtained results [6,7,8,10] are the basis for raising a number of questions about the action mechanisms of metabolites of microorganisms from Permafrost on human immunocompetent cells. Considering also that TNFα, IL-1β and IL-10 are systemic cytokines that are responsible not only for the activation of the immune system, but also for the mobilization of regulatory systems such as the nervous and endocrine [3,14,15], it is possible to consider the possibility to use secondary metabolites of Permafrost microorganisms as a substrate for the development of new immunomodulators and adaptogens.…”

Section: Resultssupporting

confidence: 52%

“…An experimental study of microorganisms (MO) of strain M3 Bacillus sp. isolated from permafrost of the late Neogene showed that a change in the temperature conditions of cultivation has a significant effect on the biological properties of bacteria in vitro and in vivo, in particular, it changes their enzymatic, immunotropic and reparative activity [6,7,8,10]. It seems relevant to study the effect of MB of MO from Permafrost on the functional activity of immunocompetent human cells in vitro for development, in the future, immunotropic drugs based on them.…”

Section: Introductionmentioning

confidence: 99%

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Influence of metabolites of microorganisms from permafrost on the synthesis cytokines by human peripheral blood mononuclear cells in vitro

Kolyvanova

Kalenova

2021

Med. immunol.

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Permafrost is a unique ecosystem characterized by consistently negative temperatures. It has been shown that microorganisms can be there in a state of hypometabolism or anabiosis during geological time. It is known that microorganisms occupy a wide habitat due to the presence of multifunctional systems of adaptation and communication. One of the manifestations of these systems is the production of secondary metabolites (MBs), which include signaling molecules that do not have strict species specificity. The biological activity of signaling molecules largely depends on the number of bacterial cells and the temperature of their cultivation.In this work we used secondary MBs of Bacillus sp. from Permafrost obtained at different temperatures of microorganism cultivation (at -5 °C – “cold” MBs and at 37 °C – “warm” MBs) in doses of 0,05 × 106 (small dose) of microbial cells (m.cl.) in ml of saline or 500 × 106 (high dose) m.cl./ml. The influence of MB of Bacillus sp. for the TNFα, IL-1β, IL-8, IL-2, IFNγ, IL-4 and IL-10 production by human peripheral blood mononuclear cells (MNC) in supernatants of 24-hour cell cultures was estimated by ELISA whith using the “VectorBEST” test system (Russia) on a LUCY-2 (ANTHOS) spectrophotometer (Austria).It was found that the activity of synthesis by human MNC of the main spectrum of cytokines significantly increased (p < 0.01 for all indicators) under the influence of MB Bacillus sp. regardless of the temperature of their cultivation and the dose of bacteria. The exception was IL-8, the level of which under the influence of a high dose of “warm” MBs didn’t differ from the control. Compared to PHA the cytokines synthesis by MNC depended on the dose and the temperature of obtaining of MBs. Thus, under the influence of “warm” MBs the level of TNFα was significantly lower than its level under the influence of PHA regardless of the dose. Regardless of the temperature of obtaining metabolites the level of IL-8 under the influence of metabolites from a dose of 500 × 106 m.cl. was reduced relative to the PHA group. Comparison of the influence of “warm” and “cold” MBs of Bacillus sp. showed that small doses of “cold” metabolites to a greater extent stimulate the synthesis of pro-inflammatory cytokines (TNFα, IL-1β, IL-8, IFNγ). High doses of “heat” metabolites of Bacillus sp. to a greater extent they activate human MNCs for the synthesis of anti-inflammatory cytokines (IL- 4 and IL-10). Considering that TNFα, IL-1β and IL-10 are cytokines of systemic action and are responsible not only for the activation of the immune system, but also for the mobilization of other regulatory systems of the organism, it can be assumed that the secondary metabolites of microorganisms from Permafrost will be efficient as a substrate for the development of new immunomodulators and adaptogens in the future.

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Section: Resultssupporting

confidence: 52%

Section: Introductionmentioning

confidence: 99%

Influence of metabolites of microorganisms from permafrost on the synthesis cytokines by human peripheral blood mononuclear cells in vitro

Kolyvanova

Kalenova

2021

Med. immunol.

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Microbiological Monitoring of Cryohydrological Geosystems of the Cryolithozone

Субботин

Петров

Гнатченко

et al. 2019

Sustainable Development of Water and Environment

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Effects of Secondary Metabolites of Permafrost Bacillus sp. on Cytokine Synthesis by Human Peripheral Blood Mononuclear Cells

et al. 2017

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We studied the effects of secondary metabolites of Bacillus sp. isolated from late Neogene permafrost on secretion of proinflammatory (TNF-α, IL-1β, IL-8, IL-2, and IFNγ) and antiinflammatory (IL-4 and IL-10) cytokines by human peripheral blood mononuclear cells. It was found that metabolites of Bacillus sp. produced more potent effect on cytokine secretion than mitogen phytohemagglutinin and metabolites of Bacillus cereus, medicinal strain IP5832. Activity of metabolites depended on the temperature of bacteria incubation. "Cold" metabolites of Bacillus sp. (isolated at -5°C) primarily induced Th1-mediated secretion of IFNγ, while "warm" metabolites (obtained at 37°C) induced Th2-mediated secretion of IL-4. The results suggest that Bacillus sp. metabolites are promising material for the development of immunomodulating drugs.

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Effects of Temperature on Biological Activity of Permafrost Microorganisms

Cited by 7 publications

References 0 publications

Influence of metabolites of microorganisms from permafrost on the synthesis cytokines by human peripheral blood mononuclear cells in vitro

Influence of metabolites of microorganisms from permafrost on the synthesis cytokines by human peripheral blood mononuclear cells in vitro

Microbiological Monitoring of Cryohydrological Geosystems of the Cryolithozone

Effects of Secondary Metabolites of Permafrost Bacillus sp. on Cytokine Synthesis by Human Peripheral Blood Mononuclear Cells

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