Effects of Temperature on Electron Transport in <i>Arum maculatum</i> Mitochondria

Cook, Neil D.; Cammack, Richard

doi:10.1104/pp.79.2.332

Cited by 11 publications

(6 citation statements)

References 12 publications

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“…It also has been suggested that increased AP capacity at iow temperature results from the fact that the AP is less temperature sensitive than is the CP (Kiener andBramlage 1981, McNulty andCummins 1987). This is supported by the fact that AP activity as a proportion of total dark respiration (TDR) is usually greater at a low measurement tetnperatiire than at a higher temperature, regardless of the ambient growth temperature (Cook andCammack 1985, Collier andCummins 1990). However, it is unclear whether the low-temperatureinduced increase in AP capacity or activity, relative to TDR,, is an acclimation response or is due to damage to the respiratory apparatus.…”

mentioning

confidence: 99%

Cytochrome and alternative pathway respiration in white spruce (Picea glauca) roots. Effects of growth and measurement temperature

Weger

Guy

1991

Physiologia Plantarum

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Interactions between growth temperature and measurement temperature were examined for their effects on white spruce [Picea glauca (Moench) Voss] root respiration. Total dark respiration rates increased with measurement temperature and were unaffected by growth temperature. Partitioning of respiratory electron flow between the cytochrome and alternative pathways was also unaffected by growth temperature. The proportion of respiration mediated by the alternative pathway was constant at measurement temperatures between 4°C and 18°C, but was increased at higher temperatures. Changes in alternative pathway activity were paralleled by changes in capacity, and the alternative pathway was almost fully engaged at all temperatures. Roots grown at low temperature displayed higher carbohydrate levels than roots grown at higher temperatures, but respiration rate was unaffected. Spruce root respiration did not appear to acclimate to growth temperature, and the alternative pathway was not preferentially engaged at low temperature.

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confidence: 99%

Cytochrome and alternative pathway respiration in white spruce (Picea glauca) roots. Effects of growth and measurement temperature

Weger

Guy

1991

Physiologia Plantarum

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“…5), strongly suggest a role for membrane fluidity. Changes in (respiratory) enzyme activities have been reported to coincide with breaks in Arrhenius plots (Cook and Cammack, 1985). Therefore, we measured the temperature dependence of DPH fluorescence anisotropy in potato tuber callus mitochondria.…”

Section: Q Diffusionmentioning

confidence: 95%

Stimulation of the Alternative Pathway by Succinate and Malate

1995

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Stimulation of the cyanide-resistant oxidation of exogenous NADH in potato (Solanum tuberosum L. cv Bintje) tuber callus mitochondria was obtained with succinate, malate, and pyruvate. Half-maximal stimulation was observed at a succinate or malate concentration of 3 to 4 mM, which is considerably higher than that found for pyruvate (0.128 mM). No effect of succinate or malate addition was found when duroquinone was the electron acceptor. Duroquinol oxidation via the alternative pathway was poor and not stimulated by organic acids. Under stimulating conditions, no swelling or contraction of the mitochondria could be observed. Conversely, variation of the osmolarity did not affect the extent of stimulation. However, the assay temperature had a significant effect: no stimulation occurred at temperatures below 16 to 20°C. Membrane fluidity measurements showed a phase transition at about 17°C. Ubiquinone reduction levels were not significantly higher in the presence of succinate and malate, but the kinetics of the alternative oxidase were changed in a way comparable to that found for the stimulation by pyruvate. At low temperatures the alternative oxidase displayed "activated" kinetics, and a role for membrane fluidity in the stimulation of the alternative pathway by carboxylic acids is suggested.In 1989 we reported that a combination of exogenous NADH and succinate resulted in respiratory rates via the Cyt pathway that were higher than the rates with the single substrates but less than the sum of the individual rates (Wagner et al., 1989). In combination both substrates contributed to oxygen uptake, but NADH oxidation was inhibited to a greater extent than succinate oxidation. When NADH was combined with succinate or malate in the presence of cyanide, oxygen uptake rates were about the sum of the rates of the substrates given separately. It appeared that succinate and malate stimulated the cyanideresistant oxidation of exogenous NADH in potato (Solanum tuberosum L.) tuber callus mitochondria and cell suspensions of Petunia hybrida (Wagner et al., 1989). Oxidation of these substrates is not a prerequisite for stimulation: in the presence of succinate and malonate, or with D-(+)malate, rates of cyanide-resistant NADH respiration are also higher than with NADH alone. Recently, more results of carboxylic acid activation of the cyanide-resistant respiration have been published, including stimulation by succinate in mitochondria from appletreated potato tubers (Lidén and Akerlund, 1993) and by pyruvate in soybean root and cotyledon mitochondria (Millar et al., 1993). The observed stimulation of DQH, oxidation by succinate and malate in apple-treated potato tubers led Lidén and Akerlund (1993) to suggest that the alternative oxidase itself might be stimulated. Evidence for the activation of the alternative oxidase by pyruvate was provided by Millar et al. (1993) and was based on measurements of the Q redox state. They showed that pyruvate stimulation of alternative respiration with succinate in soybean root mitochondria wa...

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“…McCaig and Hill (12) suggested that decreased solubility of 02 with increasing temperature may explain, in part, the declining cyanide-insensitive respiration of winter wheat mitochondria at temperatures above 17.5°C. Thus, decreased 02 availability at high temperatures due to decreased 02 solubility could be the reason why the alternative pathway accounts for a greater proportion of the respiratory potential at low temperatures than at higher temperatures (2,3,12,14).…”

Section: Discussionmentioning

confidence: 99%

“…Thus, cyanide and azide are less inhibitory to respiration at low temperatures than at high temperatures (2,3,12,14). Cold-resistant cultivars generally show a higher potential for respiratory electron flux through the alternative pathway than do cold-sensitive cultivars (1,12,14,19,20).…”

Section: Introductionmentioning

confidence: 99%

limitation of Alternative Respiratory Pathway Activity in Grapefruit Flavedo Tissue by Oxygen Availability

Purvis¹

1988

Plant Physiol.

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The capacity of the alternative respiratory pathway increased in the flavedo tissue of 'Marsh' grapefruit (Citrus paradisi Macf.) stored at 5°C for 2 weeks or longer. Elevated 02 levels during respiratory assays enhanced respiration by the tissue at 20°C but not at 5°C. At 20°C, salicylhydroxamic acid alone was inhibitory to 02 uptake only in elevated 02-In conventional Warburg studies, alternative pathway respiration may be limited by the low solubility and/or slow rate of 02 diffusion into plant tissues, such as grapefruit flavedo, and may be responsible for the apparent low utilization of the alternative pathway potential observed in other studies.Following exposures to low temperatures, the capacity of the cyanide-and azide-insensitive alternative pathway of respiration increases in the tissues of several species of plants (1-3, 6, 12, 14, 19, 20). Thus, cyanide and azide are less inhibitory to respiration at low temperatures than at high temperatures (2,3,12,14). Cold-resistant cultivars generally show a higher potential for respiratory electron flux through the alternative pathway than do cold-sensitive cultivars (1,12,14,19,20). However, except for reports that respiratory electrons are diverted to the alternative pathway at 13°C in callus tissue of chilling-sensitive Cornius stolonifera (22) and in roots of Plantago lanceolata (16), substantiating evidence that the increased potential for the alternative pathway is actually utilized at low temperature is lacking (1,2,4,6,12,14,19,20). In grapefruit, the potential for respiration via the alternative pathway is greater in the flavedo tissue of chilling-resistant interior canopy fruit than in chilling-sensitive exterior canopy fruit (14). However, it is difficult to ascertain whether the cold-resistant fruit utilizes its greater potential for the alternative pathway since inhibitors of the alternative pathway only marginally inhibit 02 uptake at chilling and nonchilling temperatures.Compared to cytochrome oxidase, the alternative oxidase has a low affinity for 0, (9,11,17) and alternative pathway respiration is inhibited at low 02 concentrations (9, 11). Yocum and Hackett (21) MATERIALS AND METHODS Grapefruit (Citrus paradisi Macf.) were harvested from the exterior canopy of trees in research plots at the University of Florida Citrus Research and Education Center, Lake Alfred. FL, and stored, unwashed, in fiberboard cartons at 5 ±+ 0.5°C. Respiratory measurements were initiated at harvest and at weekly intervals during storage. Flavedo tissue strips. 5-mm wide and 1-to 2-mm thick, were removed from the fruit and were cut into 10-mm lengths. The strips were rinsed thoroughly in deionized water, blotted with paper towels, and placed on ice until weighed.One gram of tissue was placed in Warburg vessels on moistened filter paper or in 2 ml of 50 mm K-phosphate (pH 6.5), with or without sodium azide and/or SHAM, each at 2 mm. The center wells of the Warburg vessels contained fluted filter paper wicks with 0.

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Effects of Temperature on Electron Transport in Arum maculatum Mitochondria

Cited by 11 publications

References 12 publications

Cytochrome and alternative pathway respiration in white spruce (Picea glauca) roots. Effects of growth and measurement temperature

Cytochrome and alternative pathway respiration in white spruce (Picea glauca) roots. Effects of growth and measurement temperature

Stimulation of the Alternative Pathway by Succinate and Malate

limitation of Alternative Respiratory Pathway Activity in Grapefruit Flavedo Tissue by Oxygen Availability

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