Effects of Temperature on Growth Rate of Cultured Mammalian Cells (L5178y)

Watanabe, Ikuo; Okada, S.

doi:10.1083/jcb.32.2.309

Cited by 180 publications

(80 citation statements)

References 38 publications

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“…Such small temperature changes have been shown to slightly affect cell numbers with incubation times of 1 hour. 67 Since the cells in our experiments were exposed to the temperature change for only 10 minutes, we attribute the reduction in cell viability to the magnetic actuation of the NWs. This is in agreement with the observed independence of frequency from viability, since the force exerted by the NWs on the cells was independent of the frequency (as long as the dynamic responses of the NWs could follow the field).…”

mentioning

confidence: 99%

Non-chemotoxic induction of cancer cell death using magnetic nanowires

Contreras¹,

Sougrat²,

Zaher³

et al. 2015

IJN

102

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In this paper, we show that magnetic nanowires with weak magnetic fields and low frequencies can induce cell death via a mechanism that does not involve heat production. We incubated colon cancer cells with two concentrations (2.4 and 12 μg/mL) of nickel nanowires that were 35 nm in diameter and exposed the cells and nanowires to an alternating magnetic field (0.5 mT and 1 Hz or 1 kHz) for 10 or 30 minutes. This low-power field exerted a force on the magnetic nanowires, causing a mechanical disturbance to the cells. Transmission electron microscopy images showed that the nanostructures were internalized into the cells within 1 hour of incubation. Cell viability studies showed that the magnetic field and the nanowires separately had minor deleterious effects on the cells; however, when combined, the magnetic field and nanowires caused the cell viability values to drop by up to 39%, depending on the strength of the magnetic field and the concentration of the nanowires. Cell membrane leakage experiments indicated membrane leakage of 20%, suggesting that cell death mechanisms induced by the nanowires and magnetic field involve some cell membrane rupture. Results suggest that magnetic nanowires can kill cancer cells. The proposed process requires simple and low-cost equipment with exposure to only very weak magnetic fields for short time periods.

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mentioning

confidence: 99%

Non-chemotoxic induction of cancer cell death using magnetic nanowires

Contreras¹,

Sougrat²,

Zaher³

et al. 2015

IJN

102

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“…For example, a temperature drop from 37 to 33 °C doubles the generation time of HeLa cells [50], while shifting mouse leukemia cells from 37 to 28 °C prolongs the cell cycle 7-fold [51]. While mitosis in most mammals (when defined from nuclear envelope breakdown to anaphase onset) takes ~25-30 min at 37 °C, it requires almost an hour at 33-34 °C.…”

Section: Temperature Controlmentioning

confidence: 99%

Imaging the division process in living tissue culture cells

Khodjakov

Rieder

2006

Methods

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We detail some of the pitfalls encountered when following live cultured somatic cells by light microscopy during mitosis. Principle difficulties in this methodology arise from the necessity to compromise between maintaining the health of the cell while achieving the appropriate temporal and spatial resolutions required for the study. Although the quality of the data collected from fixed cells is restricted only by the quality of the imaging system and the optical properties of the specimen, the major limiting factor when viewing live cells is radiation damage induced during illumination. We discuss practical considerations for minimizing this damage, and for maintaining the general health of the cell, while it is being followed by multi-mode or multi-dimensional light microscopy.

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“…Numbers in parenthesis repreent the franction of the total cell pupulation. ** Phase duration was calculated through conventional equations Watanabe and Okada, 1967) polyploidized by K-252a exhibiting a nuclear morphology that was mainly multi-lobed mononuclear (FujikawaYamamoto, et al, 1999b). Demecolcine (Colcemid) prevents the formation of spindle fibers in M phase and induces polyploidization and multi-nucleization in V79 cells (Fujikawa-Yamamoto et al, 1999b).…”

Section: Table I Cell Cycle Parameters Of Pseudo Diploid Andmentioning

confidence: 99%

Different Responses of Polyploidized V79 Cells after Removal of Two Drugs, Demecolcine and K-252a.

Fujikawa-Yamamoto¹,

Yamagishi²,

Zong³

et al. 2000

Cell Struct. Funct.

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ABSTRACT. To examine whether or not cells polyploidized by different mechanisms behave in a different manner after drug removal, V79 Chinese hamster cells were assessed by flow cytometry (FCM) after their polyploidization by demecolcine and K-252a, inhibitors of spindle-fiber formation and protein kinase, respectively. Cell cycle analysis of DNA histograms of V79 cells before and after the drug release was performed. With both drugs, the ploidy of V79 cells increased just after the drug removal and was maintained for a week. A difference was evident 10 days after the release. Tetraploid cells were the main population from 10 to 18 days after the release of K-252a, but not demecolcine. Cell cycle parameters were almost the same in pseudo diploid and tetraploid V79 cells, except for the tetraploid S phase which was 2h longer.Key words: cell cycle analysis/V79 cells/polyploidization/K-252a/demecolcine V79 Chinese hamster lung cells were polyploidized at the same rate by demecolcine and K-252a, inhibitors of protein kinases and spindle-fiber formation in M phase, respectively (Fujikawa-Yamamoto et al., 1993), but their morphology differed between multi-and mono-nuclei detected in the cells polyploidized by demecolcine and K-252a, respectively (Fujikawa-Yamamoto et al., 1999b). It is of interest to determine whether or not the cells polyploidized by different mechanisms behave in a different manner after the drug removal.A relationship between the rate of DNA synthesis and DNA content has been reported for Chinese hamster cells by Graves and McMillan (1984) wherein the duration of S phase is almost constant regardless of the DNA content. This was supported by the finding of increased BrdU uptake in polyploid CHO cells (Takanari et al., 1985). Although several studies have reported a constant duration of the S phase in the polyploidization of cultured cells (Brenneisen et al., 1994;Fujikawa-Yamamoto et al., 1997b;Graves et al., 1984;Jordan et al., 1996;Usui et al., 1991;Watters et al., 1994;Zhang et al., 1996), except for Meth-A cells (Fujikawa-Yamamoto et al., 1997b), the cell cycle parameters, that is, the duration of G1, S and G2/M phases, of polyploid cells in a steady state of growth have not been studied well because of difficulty in achieving such a state.In this study, the behavior of V79 cells polyploidized by demecolcine and K-252a was examined by flowcytometry (FCM) for about one month after the removal of the drugs and the cell cycle parameters determined for the pseudo diploid and tetraploid. Materials and Methods CellsV79 (Chinese hamster lung cell line) cells were maintained in a humidified atmosphere of 5% CO2 at 37°C as a monolayer culture in Leibovitz's L15 : Ham's F10 mixture (7:3) supplemented with 10% fetal calf serum (M.A. Bioproducts, Walkersville, Md, USA), streptomycin (100 µg/ml) and penicillin (50 units/ml). The cells were cultured at low density.

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Effects of Temperature on Growth Rate of Cultured Mammalian Cells (L5178y)

Cited by 180 publications

References 38 publications

Non-chemotoxic induction of cancer cell death using magnetic nanowires

Non-chemotoxic induction of cancer cell death using magnetic nanowires

Imaging the division process in living tissue culture cells

Different Responses of Polyploidized V79 Cells after Removal of Two Drugs, Demecolcine and K-252a.

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