Effects of temperature on the activity and kinetics of the granulovirus infecting the potato tuber moth Phthorimaea operculella Zeller (Lepidoptera: Gelechiidae)

Sporleder, M.; Zegarra, Octavio; Cauti, Erica Maritza Rodriguez; Kroschel, J.

doi:10.1016/j.biocontrol.2007.10.021

Cited by 28 publications

(35 citation statements)

References 22 publications

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“…The optimal temperature for survival of PTM larvae is 24 to 28°C (Briese 1980;Sporleder et al 2004). Larval survival declines at temperatures lower than 20°C due to the inability of some larvae to penetrate the potato (Briese 1980) and at 16°C natural mortality is as high as 64% (Sporleder et al 2008). Conversely, at 18°C larval mortality in control groups were similar to that achieved at higher temperatures tested herein.…”

Section: Discussionsupporting

confidence: 50%

“…Mortality of PTM larvae to PhopGV on potato tubers was not significantly influenced by temperatures from Kroschel et al 1996;Zeddam et al 1999;Sporleder 2003;Sporleder et al 2005Sporleder et al , 2007Sporleder et al and 2008. However, because of different experimental procedures (larval age, larval diet, susceptibility of PTM population, method of viral inoculation) and data analysis used in these studies, comparisons can not be applied directly.…”

Section: Discussionmentioning

confidence: 83%

See 1 more Smart Citation

Potential of a granulovirus isolate to control Phthorimaea operculella (Lepidoptera: Gelechiidae)

Mascarin¹,

Alves²,

Rampelotti-Ferreira³

et al. 2010

BioControl

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In this study a Brazilian granulovirus strain, PhopGV, isolated from the potato tuber moth (PTM) Phthorimaea operculella, was investigated regarding its potential for biological control and in vivo production. The relationship between mortality of P. operculella larvae and virus concentration was determined at different temperatures on potato tubers and susceptibility of P. operculella to PhopGV was also determined on potato leaves. Virulence of PhopGV to P. operculella was not affected by temperatures from 18 to 30°C. The median lethal concentration (LC 50 ) of larvae fed on potato foliage treated with PhopGV was not higher than that verified with larvae fed on treated tubers. Optimal conditions for production of virus-infected larvae were obtained by using the virus suspensions of 41 9 10 5 , 6.3 9 10 5 and 62 9 10 5 OBs ml -1 at 18, 24 and 30°C, which resulted in 32.0, 31.4 and 34.8% of infected larvae collected, respectively. The maximum percentage of infected larvae recovered from tubers was not affected by temperature. However, time for production of virusinfected larvae was longer at 18°C and shorter at 30°C. Persistence of PhopGV was determined on stored tubers and we observed that the virus remained effective for at least two months, causing up to 84.2% mortality of P. operculella at 1 9 10 7 OBs ml -1 . The pathogen was also highly virulent to tomato pinworm, Tuta absoluta, inflicting high percentage of mortality, delaying larval growth and inhibiting pupation. This Brazilian PhopGV strain has potential to control PTM larvae on potato tubers at a broad range of temperature and can be produced in vivo using virus-treated tubers.

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Section: Discussionsupporting

confidence: 50%

Section: Discussionmentioning

confidence: 83%

Potential of a granulovirus isolate to control Phthorimaea operculella (Lepidoptera: Gelechiidae)

Mascarin¹,

Alves²,

Rampelotti-Ferreira³

et al. 2010

BioControl

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“…Damage occurs principally in storerooms, where previously-infested tubers engender continuous generations of P. operculella and damage uninfected potatoes in storage. In the field, larvae feed on both the potato foliage and the tuber, reducing the quality of production and increasing the risk of pathogen infection (Trivedi and Rajagopal 1992;Ferro and Boiteau 1993;Sporleder et al 2008;Rondon 2010). …”

Section: Introductionmentioning

confidence: 99%

Life Table Parameters of <I>Phthorimaea operculella</I> (Lepidoptera: Gelechiidae), Feeding on Tubers of Six Potato Cultivars

Golizadeh

Razmjou

2010

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“…The relationship between log virus copies per larva and time p. i. followed a sigmoidal curve, similar to that reported for the granulovirus infecting Phthorimaea operculella Zeller (Lepidoptera: Gelechiidae) by counting total granules per larva in a dark field microscope [20]. In contrast, the trend in EpapGV granulin content per E. aporema larva over time measured by DAS-ELISA was reported to exhibit a continuous exponential increase until the end of the experiment at 5 days p. i.…”

Section: Discussionmentioning

confidence: 93%

“…However, the lack of an accurate method to quantify the number of viral copies in a given sample has impeded the investigation of different aspects of disease development. The in vivo increase in viral titer is extremely variable among baculovirus-host systems and is affected by multiple factors, such as concentration of virus inoculum, temperature and host developmental stage [10,14,15,20]. Most of these studies have focused on the NPV group, while information concerning GV is still limited.…”

Section: Introductionmentioning

confidence: 97%

Detection and kinetic analysis of Epinotia aporema granulovirus in its lepidopteran host by real-time PCR

et al. 2012

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Epinotia aporema granulovirus (EpapGV) has attracted interest as a potential biocontrol agent of the soybean pest Epinotia aporema in Argentina. Studies on virus/host interactions conducted so far have lacked an accurate method to assess the progress of virus load during the infection process. The present paper reports the development of a real-time PCR for EpapGV and its application to describe viral kinetics following ingestion of two different virus doses by last-instar E. aporema larvae. Real-time PCR was shown to be a reliable method to detect and quantify the presence of EpapGV in the analyzed samples. The increase in virus titer (log) exhibited a sigmoidal pattern, with an exponential growth phase between 24 and 48 h postinfection for both initial doses tested.

show abstract

Effects of temperature on the activity and kinetics of the granulovirus infecting the potato tuber moth Phthorimaea operculella Zeller (Lepidoptera: Gelechiidae)

Cited by 28 publications

References 22 publications

Potential of a granulovirus isolate to control Phthorimaea operculella (Lepidoptera: Gelechiidae)

Potential of a granulovirus isolate to control Phthorimaea operculella (Lepidoptera: Gelechiidae)

Life Table Parameters of <I>Phthorimaea operculella</I> (Lepidoptera: Gelechiidae), Feeding on Tubers of Six Potato Cultivars

Detection and kinetic analysis of Epinotia aporema granulovirus in its lepidopteran host by real-time PCR

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