Effects of terpinen‐4‐ol fumigation on protein levels of detoxification enzymes in Tribolium confusum

Abstract

Terpinen‐4‐ol has high fumigating activity to stored‐grain pests including Tribolium confusum. To understand the detoxification of terpinen‐4‐ol in insects, proteomic analysis was performed to identify related proteins and pathways in response to terpinen‐4‐ol fumigation in T. confusum. By using isobaric tags for relative and absolute quantitation (iTRAQ)‐based strategy, 4,618 proteins were obtained from T. confusum adults in the present study. Comparative proteomic analysis showed that 148 proteins were upregulated and 137 proteins were downregulated in beetles under the LC50 of terpinen‐4‐ol treatment for 24 hr. According to functional classifications, differentially expressed proteins (DEPs) were enriched in xenobiotic metabolism pathways. In the detoxification pathway, the levels of 25 cytochrome P450s, 5 glutathione S‐transferases, and 2 uridine diphosphate (UDP)‐glucuronosyltransferases were changed, most of which were upregulated in T. confusum exposed to terpinen‐4‐ol. The results indicated that terpinen‐4‐ol was potentially metabolized and detoxified by enzymes like P450s in T. confusum.

Production of Mono-Hydroxylated Derivatives of Terpinen-4-ol by Bacterial CYP102A1 Enzymes

“…Its properties as a fragrance ingredient are suitable for products utilizing fragrance, and terpinen-4-ol has been used in cosmetics such as perfumes, shampoos, soaps, and cosmetics [ 14 ]. Terpinen-4-ol has the potential for utilization as a pesticide, as it shows an insecticidal effect against flour beetles such as Tribolium confusum [ 15 ]. Because the demand for eco-friendly insecticides has increased to overcome various negative effects from synthetic insecticides, such as environmental destruction, researchers are attempting to replace synthetic pesticides, which trigger environmental pollution, with naturally derived compounds [ 16 , 17 ].…”