Effects of Thawing Temperature, Number of Spermatozoa and Spermatozoal Quality on Fertility of Bovine Spermatozoa Packaged in .5-ml French Straws1

Pace, M. M.; Sullivan, John J.; Elliott, F.I.; Graham, E.F.; Coulter, G.H.

doi:10.2527/jas1981.533693x

Cited by 111 publications

(61 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Reductions in the viability of individual spermatozoa will be masked by the remaining competent cells. For this reason, in a trial in which different numbers of spermatozoa were included in each insemination straw, Pace, Sullivan, Elliott, Graham & Coulter (1981) The standard errors of estimate for the non-return rate predicted using the equations above for any one ejaculate were about 10-5% so that the confidence intervals (P = 005) would be around 21%. This is inversely proportional to the square root of the number of ejaculates examined so that to improve the confidence interval to even 10% non-return would require 5 ejaculates to be examined.…”

Section: Nuclear Vacuoles and Non-return Ratementioning

confidence: 99%

Semen assessment, fertility and the selection of Hereford bulls for use in AI

Wood¹,

Foulkes²,

Shaw³

et al. 1986

Reproduction

View full text Add to dashboard Cite

Summary. Nineteen young Hereford bulls were used to study the relationship between semen characteristics and fertility in artificial insemination following 15 320 inseminations. Seven measures of sperm motility, morphological abnormalities, the release of hyaluronidase, ATP content and sperm head measurements were examined as predictors of fertility (49-day fixed-interval non-return rate). Two assessments of motility, three categories of abnormal spermatozoa, acrosomal changes and the release of hyaluronidase had predictive power. Multiple regression analysis showed that a combination of sperm motility after dilution in saline, motility after thawing and the proportion of coiled tails and proximal protoplasmic droplets provided the best prediction of fertility and allowed bulls to be ranked in order of observed non-return rate (%) with a Spearman correlation better than +0\m=.\80.

show abstract

Section: Nuclear Vacuoles and Non-return Ratementioning

confidence: 99%

Semen assessment, fertility and the selection of Hereford bulls for use in AI

Wood¹,

Foulkes²,

Shaw³

et al. 1986

Reproduction

View full text Add to dashboard Cite

show abstract

“…It can also be discussed whether it is the actual number of motile sperms inseminated, rather than the percentage of motile sperms, that is related to fertility (Saacke et al, 1991). This is also maintained by Pace et al (1981), who showed that the number of spermatozoa inseminated was positively correlated (P < 0.05) to 90-d NRR. Den Daas (1992) concluded that a successful penetration of the oocyte depends on the quality of the inseminated spermatozoa extrinsic sperm quality.…”

Section: Introductionmentioning

confidence: 55%

The impact of sperm motility on non-return rate in preselected dairy bulls

Stålhammar¹,

Janson²,

Philipsson³

1994

Reprod. Nutr. Dev.

View full text Add to dashboard Cite

Summary ― The objective of this study was to investigate whether variation in sperm motility, after preselection of bulls for this trait, has any impact on the non-return rate (NRR). The material consisted of pre-and post-freezing motility records and NRR records from 117 young Al bulls with at least 100 services each. Data representing 2 breeds at 2 different Al studs were analysed within studs. Both quadratic and linear regression analyses were performed to describe the relationship when the fixed effects of herd-year, month and order number of the insemination, parity and breed of the female, and the effects of service bull were considered simultaneously. The overall correlation with NRR was low. Motility before or after freezing never accounted for more than 1% of the variation in any of the adjusted NRR. These results showed that the remaining variation in motility was a poor predictor of NRR, which still showed a substantial variation between bulls. The significant tr values varied between 0.13 and 0.24. A motility threshold level of 50%, which is often stipulated for approval of the semen after freezing, does not seem to be the optimal choice. In order to avoid unnecessary culling, it is therefore advisable to reduce the level below which ejaculates should be rejected. The examination and selection for fertility should be completed by direct recording and evaluation of NRR. How far the motility level should be lowered to allow bulls to be tested for fertility in the field warrants investigation by utilizing semen with different degrees of reduced motility.Al bull / sperm motility / non-return rate I prediction Résumé ― Effet de la motilité du sperme de taureau laitier sur le taux de non-retour obtenu u avec des taureaux sélectionnés. Cette étude avait pour objet de voir si la variation de la motilité du sperme, après une présélection des taureaux selon ce caractère, a des conséquences sur le taux de non-retour des inséminations artificielles. Les données de motilité avant et après congéla-tion de la semence ainsi que les données de non-retour de 117 jeunes taureaux, chacun avec au moins 100 inséminations, ont été analysées. Deux races de taureaux dans 2 centres d'IA ont servi pour les analyses réalisées séparément pour chaque centre. Les relations entre la motilité et la fertilité ont été étudiées par l'analyse de régressions linéaires entre ces paramètres, en considérant simultanément les effets fixes des troupeaux et année, mois et numéro d'insémination, parité et race des femelles, et les effets des taureaux. La corrélation globale avec les taux de non-retour est faible. La motilité avant ou après congélation n'explique pas plus de 1 % de la variation des taux de nonretour, lesquels présentent encore une variation importante entre taureaux. Les valeurs significatives de b varient entre 0,13 et 0,24 ; plusieurs coefficients présentent même une valeur négative, mais non significative. Le seuil de motilité de 50%, souvent proposé pour accepter la semence après congélation, ne semble pas sati...

show abstract

“…Acrosin levels in individual ejaculates are significantly correlated with their capacity to fertilize human oocytes in vitro (Van der Ven et al 1987). A relationship between acrosin levels and fertility was also found in cattle (Pace et al 1981). Separate measurement of proacrosin and the non-zymogen (free) acrosin is probably not important from a clinical standpoint (Zaneveld et al 1990).…”

mentioning

confidence: 91%