Effects of the administration of miconazole by different routes on the biomarkers of the “steroidal module” of the Athlete Biological Passport

Abstract

This article reports the results obtained from the investigation of the influence of miconazole administration on the physiological fluctuation of the markers of the steroid profile included in the "steroidal module" of the Athlete Biological Passport.Urines collected from male Caucasian subjects before, during, and after either sys-

Annual banned‐substance review: Analytical approaches in human sports drug testing 2020/2021

“…Among the drug‐ or diet‐related confounding factors of the steroidal module of the ABP, new information was presented concerning the potential impact of synthetic isoflavones, 62 miconazole, 63 and indomethacin 64 as investigated in the context of administration studies. The isoflavones methoxyisoflavone (450 mg/day) and ipriflavone (400 mg/day) were ingested by five male volunteers, who collected six urine samples per day each.…”

“…In contrast to the aforementioned compounds, miconazole is routinely considered in doping control analyses as confounding factor of the steroidal module of the ABP 124 . In a recent study by Mazzarino et al, 63 the underlying mechanism of miconazole's effect on the analytical results imported into the ABP was shown to be based on the drug's inhibitory action on the routinely conducted enzymatic hydrolysis of steroid glucuronides. The effect was found to be concentration‐dependent as only systemic administrations resulting in urinary miconazole concentrations in excess of 0.5 μg/ml exhibited an influence on the analytical data.…”

Annual banned‐substance review 16th edition—Analytical approaches in human sports drug testing 2022/2023

“…Following a continued scientific debate, the status of thyroid hormones did not change in 2023, furnished also by prevalence study data suggesting minimal (if any) evidence for thyroid hormone abuse in Australian athletes 29 . However, the fact that liothyronine (synthetic derivative of thyroid hormone) administrations were shown to affect urinary steroid profile parameters was mentioned to justify consideration of thyroid hormone use in evaluations of the steroidal module of the athlete biological passport (ABP) 30 …”

Antibodies as a versatile tool in detection strategies for proteins: recombinant bovine Somatotropin as a model

“…Consequently, for detection methods criteria were set to assure scientifically sound interpretation of the data obtained. Criteria sets, are layed down in Commission Regulation 2021/808 [69], concerning "the performance of analytical methods for residues of pharmacologically active substances used in food-producing animals and on the interpretation of results as well as on the methods to be used for sampling". This Commission Regulation distinguishes 2 types of methods, screening-and confirmatory methods, which both have their own set of criteria to abide by.…”

“…Therefore, significant variations in biomarker profiles are used to pinpoint misuse of prohibited substance. For this the administration route, gender and training load, amongst others, are taken into account [68][69][70]. To determine deviated biomarker patterns, a harmonised, scientifically and legally robust programme on profiling of biomarkers during a longer period of time was developed [66].…”