Effects of the Antibiotics Gentamicin on the Postembryonic Development of Chrysomya putoria (Diptera: Calliphoridae)

Ferraz, Adriana Cristina Pedroso; Dallavecchia, Daniele Lourinho; Silva, Débora C.; Figueiredo, Adriana Leal de; Proença, Barbara; Silva-Filho, Renato G.; Aguiar, Valéria Magalhães

doi:10.1093/jisesa/ieu141

Cited by 8 publications

(5 citation statements)

References 28 publications

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“…The growth, weight and survival were not altered by therapeutic antibiotic doses. The development of Chrysomya putoria (Wiedemann, 1830) maggots was not altered by the antibiotic gentamicin (Ferraz et al, 2014). The examination of the effects of ceftriaxone and levofloxacin on the development of Calliphora vomitoria (Linnaeus, 1758) revealed that levofloxacin delayed the maggot development and that the pupation was delayed when both antibiotics were applied (Preußer et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Effects of antibiotics ceftriaxone and levofloxacin on the growth of Lucilia sericata (Diptera: Calliphoridae)

Preußer,

Fischer,

Juretzek

2023

Medical Vet Entomology

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Lucilia sericata is one of the most studied species in forensic entomology due to its widespread distribution, forensic importance as well as medical use. The growth and development stage of maggots is often used to determine the post‐mortem interval in forensic cases. L. sericata can cause myiasis in humans who are not able to maintain personal hygiene due to age or medical condition and can therefore be used to determine the time period of neglect. The influence of the temperature on the maggot development has been examined in various studies. Different examinations on the effects of toxic substances on the maggot development and survival have been conducted in order to test the influence and resulting deviations. In this study, the effects of different therapeutical doses of the antibiotics ceftriaxone and levofloxacin were examined on L. sericata in order to determine and compare deviations in maggot development and survival. The used antibiotics did not significantly influence the maggot weight and length. A significant delay in the time of pupation has been determined in the treatments with high concentrations of levofloxacin. The mortality was significantly increased in all treatments with antibiotics compared to the control, whereby the survivability of all treatments remained over 80%. Because of the high doses needed to produce an effect, we concluded that an overestimation of the post‐mortem interval is unlikely but higher antibiotic concentrations or possible interactions with other medications could increase the maggot development time.

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Section: Introductionmentioning

confidence: 99%

Effects of antibiotics ceftriaxone and levofloxacin on the growth of Lucilia sericata (Diptera: Calliphoridae)

Preußer,

Fischer,

Juretzek

2023

Medical Vet Entomology

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“…On this subject, Ferraz et al . (, b; ) tested the development of C . putoria using a diet‐containing antibiotics (ampicillin, gentamicin and ciprofloxacin) in different concentrations, to study the species' growth rate under the effect of these substances.…”

Section: Introductionmentioning

confidence: 99%

Post‐embryonic development of Chrysomya putoria (Diptera: Calliphoridae) in swine and bovine tissues in South America: implications for forensic entomology

2017

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Necrophagous diptera are used in forensic entomology to measure the post-mortem interval (PMI) of cadavers. PMI estimates based on fly larvae, however, can be inaccurate when larval development is retarded, or accelerated, by the nutritional composition of the cadaveric tissue. We compared the post-embryonic development of Chrysomya putoria (Wiedemann, 1830) on different tissues of pigs and cows. Forty newly hatched fly larvae per treatment were placed on 60 g of the following swine tissues: liver (T1), swine lung (T2), swine heart (T3), swine muscle (T4), bovine heart (T5), bovine lung (T6) and bovine muscle (T7). There were four replicates/treatment. Experiments were conducted in controlled conditions (28°C day/26°C night, RH 70% ± 10% and 12 h of photophase). Fly larvae reared on swine liver took longer to develop (5.2 days) than flies reared on other substrates (from 3.5 to 4.5 days), and this difference was statistically significant. The total duration of development on swine liver (T1) was significantly slower (8.7 days) when compared to the other treatments. Larvae reared on swine lungs (T2) were significantly heavier in the third instar (x = 61.2 mg) than larvae reared on swine liver (T1), swine muscle (T4), bovine lung (T6) and bovine muscle (T7). Total larval survival was high (above 76%) in all treatments except for T1 (66%). There was no deviation from the expected adult sex ratio, and the percentage of abnormalities remained within the expected range. These results reinforce the idea that, to achieve greater accuracy in PMI estimates, the type of tissue on which the larvae develops needs to be taken into account.

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“…Chronic wound debridement therapy with larvae is simple, and highly effective. It can even be used in patients undergoing antibiotic therapy without the danger of these interfering with the therapeutic action of the larvae (Ferraz et al 2014). It is indicated for different types of infected wounds and its effectiveness has already been widely proven (Sherman et al 2013, Siribumrungwong et al 2018, Borkataki et al 2018.…”

Section: Discussionmentioning

confidence: 99%

“…The mastery of breeding techniques for this species enables obtaining females with a high capacity for oviposition, generating a large amount of egg masses to be submitted to the sterilization process, which is an important prerequisite for its use in biotherapy. In addition, the possibility of storing the larvae obtained under refrigeration improves their distribution logistics for use (Dallavecchia et al 2015, Ferraz et al 2014.…”

Section: Introductionmentioning

confidence: 99%

Biological behavior of Chrysomya putoria (Wiedemann, 1819) (Diptera: Calliphoridae) after refrigeration: Logistics for use in Biotherapy

Dallavecchia

Filho

Silva

et al. 2023

An. Acad. Bras. Ciênc.

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The influence of refrigeration on the post-embryonic development of Chrysomya putoria larvae was evaluated, regarding its resistance in the logistics of storage and distribution in biotherapy. Previously sterilized larvae were submitted to four periods of storage under refrigeration (T1=12 h, T2=24 h, T3=48 h and T4=72 h) and control (without sterilization and refrigeration). Newly hatched larvae (0.200 g) were stored between 3 and 9ºC. After refrigeration, 40 neo-larvae (in triplicate) were transferred to 50 g of protein diet and incubated in an acclimatized chamber. There was a significant difference in the larval body mass (T1 and T2) and in the duration of larval, pupal and total development (T3 and T4). The sex ratios found in the four treatments did not differ from what was expected. Normality rates were 100% for all treatments.There was no significant difference between the Control, T1 and T2 treatments for larval, pupal and total viability. There was a significant difference between control (C) and T4 (larval viability), between C, T3 and T4 (pupa) and between C and T4 (total). C. putoria has resistance under refrigeration and storage of up to 56 h, presenting viability above 70% for use in biotherapy.

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Effects of the Antibiotics Gentamicin on the Postembryonic Development of Chrysomya putoria (Diptera: Calliphoridae)

Cited by 8 publications

References 28 publications

Effects of antibiotics ceftriaxone and levofloxacin on the growth of Lucilia sericata (Diptera: Calliphoridae)

Effects of antibiotics ceftriaxone and levofloxacin on the growth of Lucilia sericata (Diptera: Calliphoridae)

Post‐embryonic development of Chrysomya putoria (Diptera: Calliphoridae) in swine and bovine tissues in South America: implications for forensic entomology

Biological behavior of Chrysomya putoria (Wiedemann, 1819) (Diptera: Calliphoridae) after refrigeration: Logistics for use in Biotherapy

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