Most breast cancers originate in the ductal epithelium and are referred to as invasive ductal carcinoma. In this study we report on the clinical procedures adopted to diagnose myiasis in association with infiltrating metastatic breast carcinoma in a female patient. A 41 years old woman came to the Federal Hospital of Andaraí complaining of intense itching, warmth, redness and hardening of the breast, which had acquired the aspect of an orange peel. A lesion in the left breast was cavitated, dimpled, had fetid odor, and had fibrotic and infected air nodules filled with exudate and Dipteran larvae. The tissue was cleaned and 33 larvae were extracted. The patient was hospitalized and received Ivermectin. Eighteen of the larvae extracted from the patient were placed in 70% alcohol, and twelve were placed in a container with sterile wood shavings under controlled conditions until they metamorphosed into adults. The taxonomic identification of the flies revealed that the culprit was Cochliomyia hominivorax. A histopathological exam conducted three months earlier had revealed infiltrating ductal carcinoma. Two months after the myiasis treatment, the breast tissue had healed. The patient had waited ten days from the onset of the myiasis to seek treatment, and that delay interfered negatively in the prognosis of both the neoplasm and the myiasis. This study is relevant to public health in view of the strong social impact of myiasis.
Barbosa, L. S.; Couri, M. S.; Aguiar-Coelho, V. M. Development of Nasonia vitripennis (Walker, 1836) (Hymenoptera: Pteromalidae) in pupae of Cochliomyia macellaria (Fabricius, 1775) (Diptera: Calliphoridae), using different densities of parasitoid. Biota Neotrop., vol. 8, no. 1, Jan./Mar. 2008. Available from:
Insects display different patterns of development, and blow flies have one of the most specialized patterns of intrapuparial development of all. In forensic entomology, pupae can be used as a tool to estimate the minimum postmortem time interval (minPMI). We analyzed the intrapuparial development of Chrysomya albiceps (Diptera: Caloricidade), whose larvae had been fed pig lungs and reared in a climate-controlled room at 28°C day/26°C night, 70 ± 10% RH, and 12 h of photophase and monitored daily. After the third-instar larvae abandoned their diet, the process of pupariation and pupation was monitored. At pre-established times, five pupae were collected, euthanized, and fixed in 5% formaldehyde, inside polypropylene test tubes with caps. Since they were the first, they were classified as 0 h pupae. Twelve collections occurred until the emergence of the adults, at 0, 2, 4, 6, 8, 10, 24, 30, 48, 54, 72, 78, 96, and 99 h (n = 84). The fixed pupae were dissected under the microscope, with the aid of anatomical tweezers and hypodermic needles, and photographed. The stages of metamorphosis and the morphological alterations occurring during the process were identified, described, and recorded before and after pupation. These phases were: pupation, larval pupal apolysis, cryptocephalic, phanerocephalic, pharate adult, emergence, and adult. The cryptophalic phase occurred between 4 and 6 h after pupation; the phanerocephalic phase between 6 and 10 h after; the pharate adult phase between 24 and 96 h after; and the imago/emergence phase 99 h after pupation.
Objetivou-se descrever uma prática de ensino integrada à pesquisa que poderá ser desenvolvida tanto em escolas de ensino fundamental e médio quanto em universidades. A pesquisa foi realizada de 2002 a 2011 como atividade prática da disciplina de Parasitologia aplicada ao curso de Nutrição. Cada equipe de trabalho recebeu um roteiro prático (projeto) com as instruções para a realização da pesquisa de enteroparasitos em hortaliças. Estas foram processadas pela técnica de sedimentação espontânea utilizando-se um aparato desenvolvido com material reciclável. A observação do sedimento corado com lugol foi realizada entre lâmina e lamínula. Constatou-se contaminação em dez espécies de hortaliças com 36,4% (56/154) de positividade; destas, 48,8% para larvas de helmintos, 23,3% para cistos de protozoários e 13,9% para ovos de helmintos. A aceitação dos alunos em relação à atividade prática foi expressiva (98,4%). Esta prática vem contribuindo para a formação do acadêmico, dinamizando e incentivando o estudo da Parasitologia e alertando aos futuros profissionais da saúde sobre o fato de que a veiculação de parasitos em hortaliças consumidas in natura constitui uma questão de segurança alimentar e, consequentemente, de saúde pública. DESCRITORES: Formação acadêmica; integração ensino-pesquisa; metodologia de ensino; saúde pública; técnica de sedimentação.
Florestas tropicais são áreas que compreendem um dos ecossistemas mais ricos em espécies. A modificação destes habitats é uma das principais causas de extinção de espécies. Poucos estudos tratam da restauração ecológica a partir de estudos com a fauna silvestre, e geralmente é dada maior ênfase aos agentes polinizadores e dispersores de sementes. Este estudo, a partir de uma revisão bibliográfica, propõe uma discussão sobre indicadores ambientais e refere os mesembrinelíneos como potenciais indicadores de ambientes florestais preservados pelo seu índice de sinantropia e adaptação em áreas florestais da Região Neotropical. Um dos aspectos considerados para utilização dos insetos como indicadores ecológicos é o curto período entre suas gerações, possibilitando respostas rápidas às mudanças ambientais.
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