Insects display different patterns of development, and blow flies have one of the most specialized patterns of intrapuparial development of all. In forensic entomology, pupae can be used as a tool to estimate the minimum postmortem time interval (minPMI). We analyzed the intrapuparial development of Chrysomya albiceps (Diptera: Caloricidade), whose larvae had been fed pig lungs and reared in a climate-controlled room at 28°C day/26°C night, 70 ± 10% RH, and 12 h of photophase and monitored daily. After the third-instar larvae abandoned their diet, the process of pupariation and pupation was monitored. At pre-established times, five pupae were collected, euthanized, and fixed in 5% formaldehyde, inside polypropylene test tubes with caps. Since they were the first, they were classified as 0 h pupae. Twelve collections occurred until the emergence of the adults, at 0, 2, 4, 6, 8, 10, 24, 30, 48, 54, 72, 78, 96, and 99 h (n = 84). The fixed pupae were dissected under the microscope, with the aid of anatomical tweezers and hypodermic needles, and photographed. The stages of metamorphosis and the morphological alterations occurring during the process were identified, described, and recorded before and after pupation. These phases were: pupation, larval pupal apolysis, cryptocephalic, phanerocephalic, pharate adult, emergence, and adult. The cryptophalic phase occurred between 4 and 6 h after pupation; the phanerocephalic phase between 6 and 10 h after; the pharate adult phase between 24 and 96 h after; and the imago/emergence phase 99 h after pupation.

Prior to this work, Retrocitomyia Lopes (Diptera: Sarcophagidae) included ten recognized species distributed exclusively in the New World. We here add a new species to the genus, Retrocitomyia sisbiota sp. nov. from the Central-West Region of Brazil. Four other species of Retrocitomyia are recorded from this region for the first time, redescribed, compared, and illustrated, with emphasis on the male terminalia. Retrocitomyia paraguayensis Lopes is recorded from Brazil for the first time, R. fluminensis Lopes and R. retrocita (Hall) are newly recorded from the Brazilian states of Mato Grosso and Mato Grosso do Sul, and R. mizuguchiana Tibana & Xerez is newly recorded from the state of Mato Grosso do Sul. New generic diagnostic characters are added and the interpretation of some structures of the male terminalia is discussed. An updated catalog of all Retrocitomyia species and an identification key to the males of these species are also provided.

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A Mata Atlântica é considerada um dos 25 hotspots mundiais de biodiversidade, abrigando atualmente em suas áreas remanescentes 598 espécies ameaçadas. Para contribuir no conhecimento de algumas dessas espécies e no despertar para a conservação, propomos o álbum 'Por dentro da Mata Atlântica', como projeto do Programa de Capacitação Institucional da Difusão Científica do Instituto Nacional da Mata Atlântica. Após teste de uso e avaliação via questionário, observamos que 66,7% dos usuários foram alunos. A aparência e usabilidade foram aprovadas 81,8% e 63,6% dos usuários, respectivamente. Apenas 9,1% dos usuários revelaram não entender o conteúdo textual. As perguntas vinculadas às imagens foram compreendidas por 54,5% dos usuários. Foi considerada ferramenta educativa pelos professores e, 95,5% dos alunos gostariam de utilizar o jogo no ambiente escolar. Do total de usuários, 66,7% afirmaram que o álbum lhes acrescentou conhecimento, revelando seu potencial como ferramenta de educação ambiental e difusão científica.