Effects of the coadministration of Huatuo Zaizao pills and aspirin on hemorrheology and blood coagulation in rabbits

Biotechnology & Biotechnological Equipment

Long

et al. 2017

Compound Xueshuantong Capsule (CXC) is a traditional Chinese herbal formula composed of root of Panax pseudoginseng var. notoginseng (Burkill) G. Hoo & C.L. Tseng, root of Astragalus membranaceus var. mongholicus (Bunge) P.K. Hsiao, root of Salvia miltiorrhiza var. charbonnelii (H. L ev.) C.Y. Wu, and root of Scrophularia ningpoensis Hemsl. CXC was approved by the China Food and Drug Administration in 2003 (No. Z20030017) based on the positive clinical effect in the treatment of cardiovascular diseases. The present study was designed to elucidate the mode of action of CXC in the regulation of the haemorheology, blood coagulation, oxidative stress and energy metabolism. Rats were placed in ice-cold water (0-4 C) for 5 min during the time interval (4 h) between two adrenaline injections to induce blood circulation disorders. Blood was collected from abdominal aorta to detect whole blood viscosity, plasma viscosity, maximum platelet aggregation rate, erythrocyte aggregation index, red corpuscle electrophoresis index, activated partial thromboplastin time and prothrombin time. Myocardial tissue was homogenized to detect total antioxidant capacity, myeloperoxidase, Na +-K + ATPase, and Ca 2+ ATPase. The results indicated that, in addition to preventing haemorheological disorders, CXC could perform anti-platelet and anticoagulant effects, promote total antioxidant capacity and regulate myocardial energy metabolism, which might partly account for its mechanisms of action against cardiovascular diseases.

Section: Resultsmentioning

confidence: 61%

Protective effects of traditional Chinese herbal formula Compound Xueshuantong Capsule (CXC) on rats with blood circulation disorders

Liu

Biotechnology & Biotechnological Equipment

Long

et al. 2017

“…The slices were placed in a mixture containing 1 part of solution D, 1 part of solution E and 2 parts double distilled water or Milli-Q water. Then, Golgi Staining was performed as described previously [ 6 ]. The slides were dehydrated and cleared, and a coverslip was placed.…”

Section: Methodsmentioning

confidence: 99%

“…The Huatuo Zaizao pill (HT), a pure natural preparation derived from plants, which is a TCM formulation marketed in China [ 6 ]. HT was listed in the pharmacopoeia of the People’s Republic of China Part I (Pharmacopoeia Commission of the People’s Republic of China, 2015), as HT can be used to promote rehabilitation after stroke.…”

Section: Introductionmentioning

confidence: 99%

Huatuo Zaizao pill ameliorates cognitive impairment of APP/PS1 transgenic mice by improving synaptic plasticity and reducing Aβ deposition

BMC Complement Altern Med

Yang

et al. 2018

BackgroundIt is well known that Alzheimer’s disease (AD) is a progressive neurodegenerative disease characterized by memory deficits and cognitive decline. Amyloid-β (Aβ) deposition and synaptic dysfunction play important roles in the pathophysiology of Alzheimer’s disease (AD). The Huatuo Zaizao pill (HT) is a Traditional Chinese Medicine (TCM) that has been used clinically for many years in China, mainly for post-stroke rehabilitation and cognitive decline; however, the mechanism of cognitive function is not clear. In this study, we investigated the effect of HT on hippocampal synaptic function, Amyloid-β (Aβ) deposition in APP/PS1 AD transgenic mice.MethodSix-month-old APP/PS1 transgenic (Tg) mice were randomly divided into control, HT-treated, and memantine (MEM)-treated groups. Then, these groups were orally administered vehicle (for the control), HT (0.25 g/kg) and MEM (5 mg/kg) respectively for 4 weeks. The Morris water maze, Novel Object Recognition, and Open field tests were used to assess cognitive behavioral changes. We evaluated the effects of HT on neuronal excitability, membrane ion channel activity, and synaptic plasticity in acute hippocampal slices by combining electrophysiological extracellular tests. Synaptic morphology in the hippocampus was investigated by electron microscopy. Western blotting was used to assess synaptic-associated protein and Aβ production and degrading levels. Immunofluorescence staining was used to determine the relative integrated density.ResultsHT can ameliorate hippocampus-dependent memory deficits and improve synaptic dysfunction by reversing LTP impairment in APP/PS1 transgenic mice. Moreover, HT reduces amyloid plaque deposition by regulating α-secretase and γ-secretase levels.ConclusionHT can improve the learning and memory function of APP/PS1 transgenic mice by improving synaptic function and reducing amyloid plaque deposition.Electronic supplementary materialThe online version of this article (10.1186/s12906-018-2237-2) contains supplementary material, which is available to authorized users.

“…Despite the fact that Huatuo Zaizao pills are administered to promote the rehabilitation of stroke patients, the mechanisms of their pharmacological action are still unclear. Combined administration of Huatuo Zaizao pills and aspirin prolonged the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) and it decreased whole blood viscosity (WBV) and plasma viscosity (PV) in rabbits, potently affecting hemorrheology and blood coagulation (47). Huatuo Zaizao pills treatment increases the expression of brain-derived neurotrophic factor (BDNF) and the level of neurogenesis in animals with cerebral ischemia-reperfusion (I/R) injury, which may be associated with functional recovery after stroke (46).…”

Section: Huatuo Zaizao Pillsmentioning

confidence: 99%

Recent advances in Chinese patent medicines entering the international market

Pei

Cai

et al. 2022

DD&T

Chinese patent medicines, global acceptance, respiratory infectious diseases, cardiovascular and cerebrovascular diseases, cancers, gynecological diseases As an indispensable part of Traditional Chinese medicine (TCM), Chinese patent medicines have played an important role in preventing and treating diseases in China. Since they are easy to use, easy to store, and cost-effective, Chinese patent medicines have been generally accepted and widely used in Chinese clinical practice as a vital medical resource. In recent years, as TCM has developed and it has been accepted around the world, many Chinese patent medicine companies have gained international market access and successfully registered several Chinese patent medicines as over-thecounter (OTC) or prescription drugs in regions and countries that primarily use Western medicine such as the EU, Russia, Canada, Singapore, and Vietnam. Moreover, several Chinese patent medicines have been obtained the US Food and Drug Administration (FDA) approval conducting phase II or III clinical trials in the US. The current work has focused on several Chinese patent medicines that have been successfully registered or that have been submitted for registration abroad. Summarized here are recent advances in the efficacy and molecular mechanisms of these Chinese patent medicines to treat respiratory infectious diseases (Lianhua Qingwen capsules, Jinhua Qinggan granules, and Shufeng Jiedu Capsules), cardiovascular and cerebrovascular diseases (Compound Danshen Dripping Pills, Huatuo Zaizao pills, and Tongxinluo Capsules), cancers (a Kanglaite injection and a Shenqi Fuzheng Injection), and gynecological diseases (Guizhi Fuling Capsules). The hope is that this review will contribute to a better understanding of Chinese patent medicines by people around the world.