Effects of the combined use of glutamine and growth hormone in the intestinal adaptation after massive resection of the small bowel in rats

Spadoni, Joaquim M.; Aguilar-Nascimento, José Eduardo de; Silva, Maria H.G. Gomes da; Spadoni-Neto, Bruno; Costa, Priscila Arruda Thulio F. Batista da; Aléssio, Denise Maria T.

doi:10.1590/s0102-86502005000500008

Cited by 19 publications

(28 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Studies in animals have been investigating substances that regulate the absorptive function of the intestine 21 . These mechanisms are mediated by multiple factors, including enteral or parenteral nutrition, hormones and growth factors 22 . Recently, studies on the use of the human growth hormone (GH), the epidermal growth factor (EGF) and the glucagons-like peptide-2 (GLP-2), produced in the L-cells of the small intestine, have confi rmed them as agents that increase intestinal adaptation after massive resection 23 .…”

Section: Discussionmentioning

confidence: 99%

Biodistribution of the radiophamarceutical sodium pertechnetate (Na99mTcO4) after massive small bowel resection in rats

et al. 2007

View full text Add to dashboard Cite

Purpose:To evaluate the biodistribution of sodium pertecnetate (Na 99m TcO 4 ) in organs and tissues, the morphometry of remnant intestinal mucosa and ponderal evolution in rats subjected to massive resection of the small intestine. Methods: Twenty-one Wistar rats were randomly divided into three groups of 7 animals each. The short bowel (SB) group was subjected to massive resection of the small intestine; the control group (C) rats were not operated on, and soft intestinal handling was performed in sham rats. The animals were weighed weekly. On the 30th postoperative day, 0.l mL of Na 99m TcO 4 , with mean activity of 0.66 MBq was injected intravenously into the orbital plexus. After 30 minutes, the rats were killed with an overdose of anesthetic, and fragments of the liver, spleen, pancreas, stomach, duodenum, small intestine, thyroid, lung, heart, kidney, bladder, muscle, femur and brain were harvested. The biopsies were washed with 0.9% NaCl.,The radioactivity was counted using Gama Counter Wizard TM 1470, PerkinElmer. The percentage of radioactivity per gram of tissue (%ATI/g) was calculated. Biopsies of the remaining jejunum were analysed by HE staining to obtain mucosal thickness. Analysis of variance (ANOVA) and the Tukey test for multiple comparisons were used, considering p<0.05 as signifi cant. Results: There were no signifi cant differences in %ATI/g of the Na 99m TcO 4 in the organs of the groups studied (p>0.05). An increase in the weight of the SB rats was observed after the second postoperative week. The jejunal mucosal thickness of the SB rats was signifi cantly greater than that of C and sham rats (p<0.05). Conclusion: In rats with experimentally-produced short bowel syndrome, an adaptive response by the intestinal mucosa reduced weight loss. The biodistribution of Na 99m TcO 4 was not affected by massive intestinal resection, suggesting that short bowel syndrome is not the cause of misleading interpretation, if an examination using this radiopharmaceutical is indicated. Key words: Short Bowel Syndrome. Sodium Pertechnetate Tc 99m. Pharmacokinetics. Rat RESUMO Objetivo: Avaliar em modelo animal com ressecção extensa do intestino delgado a biodistribuição de pertecnetato de sódio (Na 99m TcO 4 ) em órgãos e tecidos, a evolução ponderal e a morfometria da mucosa do intestino delgado remanescente. Métodos: Vinte e um ratos Wistar foram aleatoriamente divididos em três grupos de sete animais cada. O grupo intestino curto (IC) foi submetido a ressecção extensa do intestino delgado, o grupo controle (C) não foi operado e o grupo sham foi submetido a leve manipulação cirúrgica das alças intestinais.Todos foram pesados semanalmente. No 30º dia pós-operatório foi administrado 0,l mL de Na 99m TcO 4 aos animais dos três grupos, IV no plexo orbital, com atividade radioativa média de 0,66MBq. Após 30 minutos os ratos foram mortos e retirados fragmentos do fígado, baço, pâncreas, estomago, duodeno, intestino delgado, tireóide, pulmão, coração, rim, bexiga, músculo, fêmur, e cérebro. As amostra...

show abstract

Section: Discussionmentioning

confidence: 99%

Biodistribution of the radiophamarceutical sodium pertechnetate (Na99mTcO4) after massive small bowel resection in rats

et al. 2007

View full text Add to dashboard Cite

show abstract

“…The sections of the intestine of C. gariepinus were routinely prepared in histopathology Laboratory of Department of Veterinary Pathology, University of Ibadan, Nigeria. Measurements of cryptal depth, villi length and width were taken using microscope with a micrometer rule as described by Spadoni et al (2005) and Eyarefe et al (2008). Four different villi were measured in each slide per parameter, recorded and an average values calculated.…”

Section: Preparation and Extraction Of Plant Materialsmentioning

confidence: 99%

The Body Weight Changes and Gut Morphometry of Clarias gariepinus juveniles on Feeds Supplemented with Walnut (Tetracarpidium conophorum) Leaf and Onion (Allium cepa) Bulb Residues

2012

View full text Add to dashboard Cite

0.05) among the treatments, the treated groups had better area of absorption and cryptal depth compared to the control. Treatment with 1.5% inclusion of walnut leaf residue recorded highest villi length and villi width and treatment with 1.5% inclusion of onion bulb recorded highest cryptal depth (30.00±0.00). These results indicate that using walnut leaf and onion bulb as a supplement in plant based diets may be useful in improving feed acceptability and growth performance of cultured C. gariepinus fed low cost, plant based diets and it was concluded that walnut (T. conophorum) leaf at 1.5% inclusion in the diet of Clarias gariepinus improved body weight gain, feed conversion ratio by increasing the absorptive capacity through increased villi length, villi width and cryptal depth.]]>

show abstract

“…Since proteins are carriers of many trace elements, rhGH may also improve protein synthesis, reduce protein breakdown, promote recovery of intestinal mucosa, increase mucosa thickness, improve intestinal barrier function, and increase absorption of trace elements [22][23][24] . In our study, the apparent absorption and utilization of zincum, cuprum and ferrum were improved in the rhGH group.…”

Section: Discussionmentioning

confidence: 99%

Effects of short-term application of low-dose growth hormone on trace element metabolism and blood glucose in surgical patients

Qian¹,

Zhi²,

WAN³

et al. 2007

WJG

View full text Add to dashboard Cite

AIM:To investigate the effects of short-term application o f l o w -d o s e g r o w t h h o r m o n e o n t r a c e e l e m e n t metabolism and blood glucose in surgical patients METHOD S : A t o t a l o f 4 8 c o n s e c u t i v e p a t i e n t s undergoing abdominal operations were randomized to receive either subcutaneous rhGH (0.15 IU/kg) or placebo (menstruum) injections daily for 7 d after surgery. The two groups had similar nutrition intake. Blood, feces, urine and drain samples were collected to measure zincum, cuprum and ferrum as well as glucose levels. Accumulative intake, excretion and balance of zincum, cuprum and ferrum, apparent absorption (AA) and apparent utilization (AU) of zincum, cuprum and ferrum, blood glucose levels and adverse events were estimated. RESULTS:There were no differences in accumulative intake and drain excretion between the two groups. The feces excretion and accumulative excretion of cuprum were lower in the rhGH group (P < 0.05). The urinary excretion of zincum, cuprum and ferrum was all significantly decreased in the rhGH group (P < 0.05) and the accumulative balance of zincum, cuprum and ferrum was improved compared with the placebo group (P < 0.05). AA of cuprum in the rhGH group was almost twice as much as the placebo group (P < 0.05), and AU of zincum, cuprum and ferrum was all improved in the rhGH group (P < 0.05). The mean blood glucose level was significantly higher in the rhGH group than in the placebo group from d 3 to d 6 after operation (P < 0.05). CONCLUSION:Postoperative low-dose rhGH treatment improves the retention of zincum, cuprum and ferrum and decreases the excretion of zincum, cuprum and ferrum, improves the balance of zincum, cuprum and ferrum, and promotes the AA and AU of zincum, cuprum and ferrum. rhGH can be well tolerated without significant adverse effects and the blood glucose level can be well controlled.

show abstract

Effects of the combined use of glutamine and growth hormone in the intestinal adaptation after massive resection of the small bowel in rats

Abstract: Gut adaptation after massive resection is improved with the combined use of glutamine and GH.

Cited by 19 publications

References 18 publications

Biodistribution of the radiophamarceutical sodium pertechnetate (Na99mTcO4) after massive small bowel resection in rats

Biodistribution of the radiophamarceutical sodium pertechnetate (Na99mTcO4) after massive small bowel resection in rats

The Body Weight Changes and Gut Morphometry of Clarias gariepinus juveniles on Feeds Supplemented with Walnut (Tetracarpidium conophorum) Leaf and Onion (Allium cepa) Bulb Residues

Effects of short-term application of low-dose growth hormone on trace element metabolism and blood glucose in surgical patients

Contact Info

Product

Resources

About