2014
DOI: 10.1111/bph.12734
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Effects of the endogenous cannabinoid anandamide on voltage‐dependent sodium and calcium channels in rat ventricular myocytes

Abstract: BACKGROUND AND PURPOSEThe endocannabinoid anandamide (N-arachidonoyl ethanolamide; AEA) exerts negative inotropic and antiarrhythmic effects in ventricular myocytes. EXPERIMENTAL APPROACHWhole-cell patch-clamp technique and radioligand-binding methods were used to analyse the effects of anandamide in rat ventricular myocytes. KEY RESULTSIn the presence of 1-10 μM AEA, suppression of both Na + and L-type Ca 2+ channels was observed. Inhibition of Na + channels was voltage and Pertussis toxin (PTX) -independent.… Show more

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Cited by 52 publications
(52 citation statements)
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“…In isolated sinoatrial node samples from rabbits, AEA shortens the action potential duration and amplitude via CB 1 (Zhang et al 2013). A similar effect of AEA, that resulted from an inhibitory effect on the functioning of voltage-dependent Na þ and L-type Ca 2þ channels, has been observed on the action potential of rat ventricular myocytes, although in these cells, the effect was independent of CB 1 and CB 2 receptors (Al Kury et al 2014). …”
Section: Endocannabinoids and Arrhythmiasmentioning
confidence: 62%
See 1 more Smart Citation
“…In isolated sinoatrial node samples from rabbits, AEA shortens the action potential duration and amplitude via CB 1 (Zhang et al 2013). A similar effect of AEA, that resulted from an inhibitory effect on the functioning of voltage-dependent Na þ and L-type Ca 2þ channels, has been observed on the action potential of rat ventricular myocytes, although in these cells, the effect was independent of CB 1 and CB 2 receptors (Al Kury et al 2014). …”
Section: Endocannabinoids and Arrhythmiasmentioning
confidence: 62%
“…This may be related to the finding that CB 1 activation in the heart decreases noradrenaline release, so less β 1 receptors will then be activated (Molderings et al 1999). It is also related to the fact that AEA inhibits the function of voltage-dependent Na þ and L-type Ca 2þ channels in rat ventricular myocytes (Al Kury et al 2014). AEA causes endotheliumdependent vasorelaxation of rat or sheep coronary arteries via CB 1 activation, with no role for endocannabinoid metabolites, CB 2 or TRPV1 (White et al 2001;Ford et al 2002;Grainger and Boachie-Ansah 2001).…”
Section: Endocannabinoids and Cardiac Functionmentioning
confidence: 99%
“…Therefore antagonism of a constitutive GPR55 activity can mimic the actions of CBD on the contractility of cardiomyocytes. However, AM251, an agonist of GPR55 and antagonist/inverse agonist of CB1 receptor, does not alter the contractility of cardiomyocytes and amplitudes of L-type Ca 2+ currents [28,29] suggesting that antagonism of GPR55 receptors does not mediate the effects of CBD in cardiomyocytes. In several recent studies, activation of peroxisome proliferator-activated receptor gamma (PPAR␥) has been shown to mediate the effects of CBD [13,30].…”
Section: Discussionmentioning
confidence: 99%
“…Anandamide, an endogenous cannabinoid is known to inhibit the ventricular voltage-dependent sodium and calcium ion channel independent of CB1 and CB2 receptor activation [21]. Anandamide inhibits INA current in neurons [22] and Xenopus oocytes [23], and no evidence exist suggesting involvement of cardiac sodium channels.…”
Section: Discussionmentioning
confidence: 99%