Effects of the in ovo injection of 25-hydroxycholecalciferol on the yolk and serum characteristics of male and female broiler embryos ,

Abstract

Serum concentrations of 25-hydroxycholecalciferol [25(OH)D3] in broiler embryos at 19 d of incubation:doi:have been shown to increase 3 times by the in ovo injection of 0.60 μg 25(OH)D3 on 18 doi. In this trial, effects of the injection of 25(OH)D3 at 18 doi on the yolks and sera of male and female Ross×Ross 708 broiler embryos were assessed. On 18 doi, embryonated eggs that were set in a single stage incubator were assigned to 6 replicate trays within each of 2 injection treatment groups. Treatments included those injected with 100 μL commercial diluent (control) and those injected with 0.60 μg 25(OH)D3 in 100 μL commercial diluent. On 19 doi, embryos and their yolk sacs were extracted for determination of sex, yolk weight, BW, serum CA and phosphorous concentrations, and yolk CA, phosphorous, moisture, dry matter, and lipid concentrations. The weight of female embryos with their attached yolk sacs as a percentage of set egg weight was greater than that of males (P=0.03). There were treatment×sex interactions for the weight of embryos with their attached yolk sacs relative to 19 doi egg weight (P=0.05) and for yolk CA concentration (P=0.004). In eggs that received 25(OH)D3, the weight of female embryos with their attached yolk sacs relative to 19 doi egg weight was higher than that of males (P=0.005), and percentage yolk CA was higher in control eggs containing female embryos in comparison to those containing males (P=0.007). An injection of 0.60 μg 25(OH)D3 at 18 doi eliminated yolk CA differences associated with embryo sex, suggesting that 25(OH)D3 may influence sex-related differences in the rate of yolk CA absorption by broiler embryos and may be related to subsequent sex-related differences in posthatch bone strength.

In ovo feeding with 25-hydroxycholecalciferol influences bone mineral density of chicks

“…The lack of treatment effects on hatchability and mortality indicates that in ovo feeding with 25(OH)D3 at the eighth day of incubation did not result in compromised embryo development. Similar to the present study, Bello et al (2015) found no difference in EML with in ovo feeding with 0.6 µg of 25(OH) D3 at 18 days of incubation. However, Sgavioli et al (2015) reported a decrease in egg hatchability after injecting increasing levels of vitamin C before incubation.…”

“…Studies have shown that in ovo inoculation of 1.25(OH)2D3, 25(OH)D3, and 24.25(OH)2D3 during incubation improves hatching, bone development, and yolk calcium concentration and interferes with receptor mRNA expression by IGF-1 (Yair et al, 2013;Bello et al, 2014a,b,c;Bello et al, 2015;Yair et al, 2015).…”

Effects of source and level of in ovo-injected vitamin D3 on the hatchability and serum 25-hydroxycholecalciferol concentrations of Ross 708 broilers

“…Effects of the in ovo injection of 25OHD 3 alone at various dosage levels on the hatchability, hatching chick quality, and the posthatch production and performance of broilers have been investigated ( Gonzales et al., 2013 , Bello et al., 2013 , Bello et al., 2014a , Bello et al., 2014b , Bello et al., 2014c , Bello et al., 2015 , Mansour et al., 2017 ). However, those effects on the broiler embryo have not been investigated when D 3 is administrated at 18 doi by amniotic in ovo injection alone or in combination with 25OHD 3 .…”

“…The in ovo injection of 0.60 μg of 25OHD 3 has likewise been shown to influence the hatchability and yolk characteristics of broilers ( Bello et al., 2013 , Bello et al., 2015 ). Embryos that received an in ovo injection of 0.60 μg of 25OHD 3 exhibited higher hatchability and serum 25OHD 3 levels than embryos from a diluent-injected control group ( Bello et al., 2013 ).…”