Effects of the Lipid Peroxidation Products 4-Hydroxy-2-Nonenal and Malondialdehyde on the Proliferation and Morphogenetic Development of in vitro Plant Cells

Adams, Linda Kay; Benson, Erica E.; Staines, Harry; Bremner, David H.; Millam, S.; Deighton, Nigel

doi:10.1016/s0176-1617(99)80120-5

Cited by 17 publications

(17 citation statements)

References 29 publications

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“…Increased levels of lipid peroxidation similar to our observations in saffron somatic embryos has been reported by Deighton et al (1997) and Adams et al (1999) in cell cultures of carrot, during the differentiation of somatic embryos where increased levels of lipid peroxidation products were detected in embryogenic cultures compared to non embryogenic ones. It could be possible that lipid peroxidation products synthesized by embryogenically competent cultures exert both cytotoxic and cytoactive effects during the progressive developmental stages of somatic embryogenesis (Benson et al 1992;Weber 2000).…”

Section: Discussionsupporting

confidence: 91%

Somatic embryogenesis in saffron (Crocus sativus L.). Histological differentiation and implication of some components of the antioxidant enzymatic system

Blázquez¹,

Olmos

Hernández

et al. 2009

Plant Cell Tiss Organ Cult

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The ontogenetic developmental stages of saffron somatic embryogenesis have been studied and characterized using light microscopy and the biochemical determination of the antioxidant enzymatic system. The embryogenic callus underwent internal segmented divisions with the formation of globular embryos that were attached to the callus surface by a broad multicellular structure. Further development of the embryoids was characterized by the emergence of a shoot apical meristem and cotyledon (monopolar stage) with the subsequent differentiation of a minicorm at the basal part of the somatic embryo (dipolar stage). During the morphological differentiation of the somatic embryos changes in the antioxidant enzymatic system with increased superoxide dismutase (SOD) and catalase (CAT) activities were detected at the initial stages of somatic embryogenesis. The isoforms of SOD, including two Mn-SODs and four Cu, Zn-SODs, were also detected. Although all the isoforms were expressed during the successive stages of somatic embryogenesis, an increase in Mn-SODs and a decrease in Cu, Zn-SODs during the last two stages was observed. Significant changes were also detected in the antioxidant activities ascorbate peroxidase, dehydroascorbic acid reductase and glutathione reductase.

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Section: Discussionsupporting

confidence: 91%

Somatic embryogenesis in saffron (Crocus sativus L.). Histological differentiation and implication of some components of the antioxidant enzymatic system

Blázquez¹,

Olmos

Hernández

et al. 2009

Plant Cell Tiss Organ Cult

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“…The author has, over several years, applied the concepts of animal free radical stress physiology and aging to the study of in vitro plants (Adams et al, 1999;Benson, 1990Benson, , 1991Roubelakis-Angelakis, 1992, 1994;Benson et al, 1992Benson et al, a,b, 1997aRobertson et al, 1995). The objective of this paper is to provide an overview of current progress and to explore the possibility that free radicals and their reaction products have a role in plant tissue culture recalcitrance.…”

Section: Introductionmentioning

confidence: 99%

Special symposium: In vitro plant recalcitrance do free radicals have a role in plant tissue culture recalcitrance?

Benson

2000

In Vitro Cell.Dev.Biol.-Plant

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119

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Free radicals have an important role in the metabolism and development of aerobic organisms; however, their uncontrolled production leads to oxidative stress. This paper explores the possibility that free radical-mediated stress has a role in tissue culture recalcitrance. In the context of this paper, recalcitrance is considered to be the inability of plant tissue cultures to respond to culture manipulations; in its broadest terms, this study also concerns the time-related decline (i.e. in vitro aging) and loss of morphogenetic competence and totipotent capacity. Studies on a diverse range of in vitro plant systems have shown that tissue cultures produce free radicals, lipid peroxides and toxic, aldehydic lipid peroxidation products. Levels of these compounds vary in response to different tissue culture manipulations, but their production is enhanced during dedifferentation and antioxidant profiles also vary throughout different phases of culture. A hypothesis is presented which suggests that tissue culture manipulations cause major metabolic and developmental changes, some of which may predispose in vitro cultures to increased free radical formation. If antioxidant protection is compromised, oxidative stress ensues and free radicals and their reaction products react with macromolecules such as DNA, proteins and enzymes, causing cellular dysfuction and, as a result, the cultures become recalcitrant.

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“…Benson 2000, Papadakis and Roubelakis-Angelakis 2002, Dutta Gupta and Datta 2003. This suggestion was confirmed by experiments with Daucus carota where application of lipid peroxidation products 4-hydroxy-2-nonenal or malondialdehyde (MDA) inhibited proliferation and embryogenesis (Adams et al 1999). Further, addition of antioxidants polyvinylpolypyrrolidone and dithiotreitol to Pinus virginiana cultures inhibited tissue necrosis and improved callus formation, shoot differentiation and growth, and rooting (Tang et al 2004a).…”

Section: In Vitro Culturementioning

confidence: 77%

Production of reactive oxygen species and development of antioxidative systems during in vitro growth and ex vitro transfer

Baťková¹,

Pospíšilová²,

Synková³

2008

Biologia plant.

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Ex vitro transfer is often stressful for in vitro grown plantlets. Water stress and photoinhibition, often accompanying the acclimatization of in vitro grown plantlets to ex vitro conditions, are probably the main factors promoting production of reactive oxygen species (ROS) and in consequence oxidative stress. The extent of the damaging effects of ROS depends on the effectiveness of the antioxidative systems which include low molecular mass antioxidants (ascorbate, glutathione, tocopherols, carotenoids, phenols) and antioxidative enzymes (superoxide dismutase, ascorbate peroxidase, catalase, glutathione reductase, monodehydroascorbate reductase, dehydroascorbate reductase). This review is focused on ROS production and development of antioxidative system during in vitro growth and their further changes during ex vitro transfer.

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Effects of the Lipid Peroxidation Products 4-Hydroxy-2-Nonenal and Malondialdehyde on the Proliferation and Morphogenetic Development of in vitro Plant Cells

Cited by 17 publications

References 29 publications

Somatic embryogenesis in saffron (Crocus sativus L.). Histological differentiation and implication of some components of the antioxidant enzymatic system

Somatic embryogenesis in saffron (Crocus sativus L.). Histological differentiation and implication of some components of the antioxidant enzymatic system

Special symposium: In vitro plant recalcitrance do free radicals have a role in plant tissue culture recalcitrance?

Production of reactive oxygen species and development of antioxidative systems during in vitro growth and ex vitro transfer

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