Effects of the mycelial extract of cultured Cordyceps sinensis on in vivo hepatic energy metabolism and blood flow in dietary hypoferric anaemic mice

Abstract

The beneficial effects of a traditional Chinese medicine, Cordyceps sinensis (Cs), on mice with hypoferric anaemia were evaluated by NMR spectroscopy. Experimental hypoferric anaemia was induced in mice by feeding with an Fe-free diet for 6 weeks. They were then given extract from cultured Cs (200 mg/kg body weight daily, orally) and were placed on an Fe-containing recovery diet (35 mg Fe/kg diet) for 4 weeks. In vivo 31 P and 2 H NMR spectra acquired noninvasively and quantitatively at weekly intervals were used to evaluate hepatic energy metabolism and blood flow in the mice. During the 4-week Cs-extract treatment, consistent increases were observed in liver b-ATP : inorganic phosphate value by liver 31 P NMR spectroscopy, representing the high energy state, and in blood-flow rate as determined by 2 H NMR spectroscopy of deuterated water (D 2 O) uptake after intravenous injection of D 2 O. The haematological variables (the packed cell volume and the haemoglobin level) and the hepatic intracellular pH, which was determined from the NMR chemical shift difference between the inorganic phosphate peak and the a-phosphate peak of ATP, were not significantly different between Cs-extract-treated and control mice. As blood flow and energy metabolism are thought to be linked, the Cs-extract-increased hepatic energy metabolism in the dietary hypoferric anaemic mice was concluded to be due to increased hepatic blood flow. Nuclear magnetic resonance: Energy metabolism: Chinese medicine

Effect of Cs-4® (Cordyceps sinensis) on Exercise Performance in Healthy Older Subjects: A Double-Blind, Placebo-Controlled Trial

“…Another study using water-soluble extracts of Cs-4 also showed an increase of 45-55% in ATP:Pi ratios in the liver of mice treated with Cs-4 compared with placebo. 22 More recently, Manabe et al, 23 confirming their earlier findings, suggested that any increase in hepatic energy metabolism might be due to increased hepatic blood flow. Whatever the mechanism, if a similar improvement in cellular energy state occurred in muscle, this suggests a potential mechanism for the changes in aerobic performance observed in the present study.…”

Preventive Effects of <i>Ophiocordyceps </i><i>s</i><i>inensis </i>Mycelium on High-Fat Diet Induced Lipid Dysregulation and Hepatic Inflammation of Mice

“…Probably, the decreased liver TG levels by OSM treatment may be related to the energy expenditure in hepatic or other tissues like skeletal muscle [9]. It has been proclaimed the Cordymin, a compound in OS, could enhance the mitochondria biogenesis in livers [40,41]. On the other hand, OS supplementation on HFDfed rats can enhance the protein expression of uncoupling protein 2 (UCP-2), further increasing energy expenditure in electronic transport chain, and then attenuate the steatosis [42].…”

The Liquid Culture Filtrates of Entomogenous fungus Paecilomyces tenuipes and Its Glycoprotein Constituent Protects against Anemia in Mice Treated with 5-Fluorouracil

“…The column was subsequently eluted with H 2 O (500 ml) to afford 100 fractions of 5 ml and the absorbance was monitored at 220 nm. After lyophilization, some of the eluted fractions (fractions [20][21][22][23][24][25] were combined and lyophilized to give Fraction II (PGF: 31.9 mg). This method effectively extracted PGF, which represented the active fraction isolated from PTCF.…”