Effects of the mycotoxin metabolite de‐epoxy‐deoxynivalenol (DOM‐1) on embryo development and sperm motility in cattle

Guerrero-Netro, Hilda M; Barreta, Marcos Henrique; Costa, Eduardo Paulino da; Goetten, André Lucio Fontana; Dupras, Robert; Mills, Louis; Koch, Juliane; Portela, Valério Marques; Price, Christopher A.; Chorfi, Younès

doi:10.1002/jat.4102

Cited by 10 publications

(12 citation statements)

References 31 publications

(40 reference statements)

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“…Reproductive studies in dairy cattle are scarce, especially those performed in the field or in in vivo controlled experimental trials with realistic contamination levels of mycotoxins (Table 3). When cows were exposed to DOM-1 via intrafollicular injection (100 ng/mL), a decrease in the follicular development was observed [173]. These authors also exposed cows to DON via feed (3 or 6 ppm) and detected serum levels of DOM-1 10 to 20 times higher than those of DON, reaching 10 to 20 ng/mL.…”

Section: Effect Of Fusarium Toxins On Reproductive System 101 In Vivo...mentioning

confidence: 94%

Adverse Effects of Fusarium Toxins in Ruminants: A Review of In Vivo and In Vitro Studies

Gallo

Mosconi

Trevisi

et al. 2022

Dairy

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With an increased knowledge of the mechanism of action of Fusarium mycotoxins, the concept that these substances are deleterious only for monogastric species is obsolete. Indeed, most mycotoxins can be converted into less toxic compounds by the rumen microflora from healthy animals. However, mycotoxin absorption and its conversion to more toxic metabolites, as well as their impact on the immune response and subsequently animal welfare, reproductive function, and milk quality during chronic exposure should not be neglected. Among the Fusarium mycotoxins, the most studied are deoxynivalenol (DON), zearalenone (ZEN), and fumonisins from the B class (FBs). It is remarkable that there is a paucity of in vivo research, with a low number of studies on nutrient digestibility and rumen function. Most of the in vitro studies are related to the reproductive function or are restricted to rumen incubation. When evaluating the production performance, milk yield is used as an evaluated parameter, but its quality for cheese production is often overlooked. In the present review, we summarize the most recent findings regarding the adverse effects of these mycotoxins with special attention to dairy cattle.

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Section: Effect Of Fusarium Toxins On Reproductive System 101 In Vivo...mentioning

confidence: 94%

Adverse Effects of Fusarium Toxins in Ruminants: A Review of In Vivo and In Vitro Studies

Gallo

Mosconi

Trevisi

et al. 2022

Dairy

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“…The reduced pregnancy rates are associated with the negative effects of mycotoxins which are possibly attributable to a toxic mechanism (Duffield, 2000;Minervini et al, 2001). Guerrero-Netro et al (2020) studied the effect of naturally contaminated feed on superovulatory response in cows and found that 6 ppm DON did not alter the number of viable embryos recovered on day 7 but de-epoxy deoxynivalenol (DOM-1) reduced the motility of spermatozoa in vitro. Moreover, Yousef et al (2017) reported that ZEA disrupted the normal interaction between sperm and bovine oviductal epithelial cells, hence negatively affecting sperm survival.…”

Section: Discussionmentioning

confidence: 99%

Fate of Milk Yield and Fertility Parameters in Holstein Dairy Cows Simultaneously Exposed to Zearalenone, Deoxynivalenol and Fumonisin B1 Mycotoxicity

Uyarlar,

Yilmaz

2024

THE JAPS

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This study was planned to investigate the effect of zearalenone (ZEA), deoxynivalenol (DON) and Fumonisin B1 (FB1) on blood metabolites, milk yield, estrous activity, pregnancy rate and uterine health in 486 heads of Holstein dairy cows. For this purpose, the data collected from the animals were divided into the four periods as follows: feeding with noncontaminated diet for 30 days (pre-contamination period), contaminated diet for 60 days (contamination period), contaminated diet supplemented with mycotoxin adsorbent for 30 days (adsorbent period) and the diet after removing the contaminated feedstuffs for 30 days (non-contamination period). Each feedstuff was analysed in contamination period and at the beginning of the non-contamination period to determine the presence or absence of ZEA, DON and FB1 in the diet. The blood metabolic profile was assessed in 21 animals in 1-10 days in milk, selected through cluster random sampling method while milk yield, estrous activity, pregnancy rate and prevalence of metritis/endometritis were compared among all four periods. The milk yield (P≤0.001) and milk fat (P≤0.05) in the contaminated period were low as compared to other periods. The concentrations of beta-hydroxybutyric acid (p≤0.001), non-esterified fatty acids (P≤0.05), gamma-glutamyl transferase (P≤0.05), and blood urea nitrogen (P≤0.001) parameters were significantly higher, while the glucose (P≤0.001) and triglycerides (P≤0.05) were lower in the contamination period, as compared to adsorbent and non-contamination periods. The pregnancy rate through artificial insemination decreased significantly during the contamination period (P≤0.05) as compared to pre-contamination period. The overall pregnancy rate in the pre-contamination period was also significantly decreased as compared to the adsorbent and non-contamination periods (P≤0.001). The percentage of type III anestrus were significantly lower during the non-contamination period (P≤0.05). The percentages of cows with metritis during the non-contamination period was significantly lower (p≤0.05) as compared to the contamination period. It was concluded that the routine feed analysis was necessary to determine the presence of ZEA, DON and FB1. Moreover, mycotoxin binder supplementation and/or removing contaminated feedstuffs from the diet under mycotoxicosis conditions may prevent the severe negative energy balance and improve the milk yield and fertility parameters.

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“…The mycotoxin metabolite DOM-1 is generally considered to be relatively inert but has been shown to be active in the ovary and intestine [ 20 , 21 , 22 ]. As DOM-1 lacks the epoxy group necessary for initiating the RSR, the mechanism of action of DOM-1 was unclear.…”

Section: Discussionmentioning

confidence: 99%

“…Ruminants are resistant to the toxic effects of DON as most ingested DON is metabolized in the rumen to de-epoxy-DON (DOM-1) [ 16 ], which is considerably less toxic than DON and does not activate the RSR [ 17 , 18 , 19 ]. However, DOM-1 is not inert; it has been shown to increase the immune response in pigs in vivo [ 20 ], to decrease follicle development in vivo in cattle [ 21 ], and to increase apoptosis of bovine ovarian theca cells in vitro [ 22 ]. The mechanism of action of DOM-1 is unclear, as although it stimulated EIF2AK2 and MAPK3/1 phosphorylation in theca cells, it also stimulated the expression of the endoplasmic reticulum stress marker ATF4 [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

The Mycotoxin De-Epoxy-Deoxynivalenol (DOM-1) Increases Endoplasmic Reticulum Stress in Ovarian Theca Cells

Reyes-Perea

Guerrero-Netro

Meza-Serrano

et al. 2023

Toxins

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Deoxynivalenol (DON) is a major mycotoxin present in animal feed and negatively affects growth and reproduction in farm species, including pigs and cattle. The mechanism of DON action involves the ribotoxic stress response (RSR), and it acts directly on ovarian granulosa cells to increase cell death. In ruminants, DON is metabolized to de-epoxy-DON (DOM-1), which cannot activate the RSR but has been shown to increase cell death in ovarian theca cells. In the present study, we determined if DOM-1 acts on bovine theca cells through endoplasmic stress using an established serum-free cell culture model and to assess whether also DON activates endoplasmic stress in granulosa cells. The results show that DOM-1 increased the cleavage of ATF6 protein, increased the phosphorylation of EIF2AK3, and increased the abundance of cleaved XBP1 mRNA. Activation of these pathways led to an increased abundance of mRNA of the ER stress target genes GRP78, GRP94, and CHOP. Although CHOP is widely associated with autophagy, inhibition of autophagy did not alter the response of theca cells to DOM-1. The addition of DON to granulosa cells partially increased ER stress pathways but failed to increase the abundance of mRNA of ER stress target genes. We conclude that the mechanism of action of DOM-1, at least in bovine theca cells, is through the activation of ER stress.

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Effects of the mycotoxin metabolite de‐epoxy‐deoxynivalenol (DOM‐1) on embryo development and sperm motility in cattle

Cited by 10 publications

References 31 publications

Adverse Effects of Fusarium Toxins in Ruminants: A Review of In Vivo and In Vitro Studies

Adverse Effects of Fusarium Toxins in Ruminants: A Review of In Vivo and In Vitro Studies

Fate of Milk Yield and Fertility Parameters in Holstein Dairy Cows Simultaneously Exposed to Zearalenone, Deoxynivalenol and Fumonisin B1 Mycotoxicity

The Mycotoxin De-Epoxy-Deoxynivalenol (DOM-1) Increases Endoplasmic Reticulum Stress in Ovarian Theca Cells

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